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Osteogenic induction and 1,25-dihydroxyvitamin D-3 oppositely regulate the proliferation and expression of RANKL and the vitamin D receptor of human periodontal ligament cells
Tang, Xiaolin1; Meng, Huanxin2
关键词Human periodontal ligament cell Osteogenic induction 1,25-dihydroxyvitamin D-3 Receptor activator of NF-KappaB ligand Osteoprotegerin Vitamin D receptor
刊名ARCHIVES OF ORAL BIOLOGY
2009-07-01
DOI10.1016/j.archoralbio.2009.04.009
54期:7页:625-633
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者National Science Foundation of China ; National Postdoctoral Fund of China ; National Science Foundation of China ; National Postdoctoral Fund of China
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]KAPPA-B LIGAND ; NODULES FORMED INVITRO ; GENE-EXPRESSION ; IN-VITRO ; OSTEOCLAST DIFFERENTIATION ; INHIBITORY FACTOR ; OSTEOPROTEGERIN ; ACTIVATOR ; GINGIVAL ; PROMOTER
英文摘要

Objective: Human periodontal ligament cells (hPDLCs) may play an important role in osteoclastogenesis in alveolar bone by expressing the receptor activator of NF-KappaB ligand (RANKL) and osteoprotegerin (OPG). The present study aimed to investigate the differences between the effects of osteogenic induction and 1,25-dihydroxyvitamin D-3 (VD3) on hPDLC proliferation and the expression of RANKL, osteoprotegerin, and the vitamin D receptor (VDR) in hPDLCs.

Methods: Primary cultures of 11 hPDLC populations from 11 donors were obtained. Three samples of each hPDLC population from passage 3 were, respectively, treated with osteogenic induction medium, 10(-8) M VD3, or vehicle as a control. Cell proliferation at days 0, 1, 3, 5, and 7 was estimated with the MTT method. At day 6, the mRNA levels of RANKL, CPG and VDR were determined with real-time RT-PCR.

Results: Osteogenic induction significantly promoted hPDLC proliferation, while VD3 inhibited proliferation. Osteogenic induction significantly down-regulated the mRNA level of RANKL by 1.61-fold (P = 0.033) and decreased the level of VDR by 2.13-fold (P = 0.003), while there was no change in the level of OPG and OPG/RANKL ratio with osteogenic induction. on the contrary, VD3 significantly up-regulated the level of RANKL by 9.58-fold (P = 0.001) and increased the level of VDR by 3.15-fold (P = 0.004), while down-regulating the OPG/RANKL ratio by 7.14-fold (P = 0.004).

Conclusion: Osteogenic induction and VD3 exert opposite effects in regulating hPDLC proliferation and mRNA expression of RANKL and VDR. This may induce hPDLCs to play different roles in alveolar bone metabolism. (C) 2009 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号30772420
资助者National Science Foundation of China ; National Postdoctoral Fund of China ; National Science Foundation of China ; National Postdoctoral Fund of China
WOS记录号WOS:000267480800003
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59958
专题北京大学口腔医学院_牙周科
作者单位1.China Med Univ, Sch Stomatol, Dept Periodontol, Shenyang 110002, Liaoning Prov, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China
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GB/T 7714
Tang, Xiaolin,Meng, Huanxin. Osteogenic induction and 1,25-dihydroxyvitamin D-3 oppositely regulate the proliferation and expression of RANKL and the vitamin D receptor of human periodontal ligament cells[J]. ARCHIVES OF ORAL BIOLOGY,2009,54(7):625-633.
APA Tang, Xiaolin,&Meng, Huanxin.(2009).Osteogenic induction and 1,25-dihydroxyvitamin D-3 oppositely regulate the proliferation and expression of RANKL and the vitamin D receptor of human periodontal ligament cells.ARCHIVES OF ORAL BIOLOGY,54(7),625-633.
MLA Tang, Xiaolin,et al."Osteogenic induction and 1,25-dihydroxyvitamin D-3 oppositely regulate the proliferation and expression of RANKL and the vitamin D receptor of human periodontal ligament cells".ARCHIVES OF ORAL BIOLOGY 54.7(2009):625-633.
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