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学科主题: 基础医学
题名:
In vitro study of HIF-1 activation and VEGF release by bFGF in the T47D breast cancer cell line under normoxic conditions: involvement of PI-3K/Akt and MEK1/ERK pathways
作者: Shi, YH; Wang, YX; Bingle, L; Gong, LH; Heng, WJ; Li, Y; Fang, WG
关键词: hypoxia-inducible factor 1 alpha ; angiogenesis ; VEGF ; bFGF ; breast tumour
刊名: JOURNAL OF PATHOLOGY
发表日期: 2005-03-01
DOI: 10.1002/path.1734
卷: 205, 期:4, 页:530-536
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Pathology
研究领域[WOS]: Oncology ; Pathology
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; HYPOXIA-INDUCIBLE FACTOR-1-ALPHA ; TUMOR ANGIOGENESIS ; FACTOR RECEPTOR ; FACTOR 1-ALPHA ; EXPRESSION ; FIBROBLAST ; INDUCTION ; MODULATION ; FACTOR-2
英文摘要:

Hypoxia-inducible factor (HIF) is critical in the modulation of tumour angiogenesis in response to hypoxia. In the present study, the mechanisms underlying basic fibroblast growth factor (bFGF)-induced activation of HIF-1 and the subsequent release of vascular endothelial growth factor (VEGF) in a human breast cancer cell line (T47D) under normoxic conditions were explored. The data show that HIF-1 alpha expression is induced by bFGF in a dose- and time-dependent fashion, while increased HIF-1 alpha protein expression and transactivity of HIF-1 are due to the phosphorylation of Akt by bFGF, as indicated by application of the phosphatidylinositol 3-kinase (PI-3K) inhibitor LY294002. The data also show that the MEK1 (mitogen-activated protein kinase kinase-1)/ERK (extracellular signal-regulated kinase) pathway is only involved in bFGF-induced transactivity of HIF-1, but not HIF1 alpha expression, indicating roles for both the PI-3K/Akt and the MEK1/ERK pathways in bFGF activity. In addition, the translation inhibitor cycloheximide confirmed that bFGF-induced HIF-1 alpha protein expression was due to de novo protein synthesis. In contrast, p38 was not required for the expression of HIF-1 alpha or HIF-1 transactivity, although significant phosphorylation of p38 was observed after bFGF treatment. Treatment of the cells with bFGF increased the amount of VEGF release, and this could be suppressed by either PD98059 or LY294002, suggesting the presence of a HIF-1 alpha-dependent pathway for bFGF-induced VEGF production. In conclusion, the PI-3K/Akt and MEK1/ERK pathways, in a potentially independent and co-operative fashion, can modulate HIF-1 activation by bFGF. Further studies will pinpoint whether HIF-1 is the transcriptional factor responsible for the increased VEGF production following bFGF treatment of breast tumour cells. Copyright (C) 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley H Sons, Ltd.

语种: 英语
WOS记录号: WOS:000227505600015
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59990
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
2.Inner Mongolia Med Coll, Dept Pathol, Huhhot, Mongol Peo Rep
3.Univ Sheffield, Sch Med, Div Genom Med, Sheffield S10 2TN, S Yorkshire, England

Recommended Citation:
Shi, YH,Wang, YX,Bingle, L,et al. In vitro study of HIF-1 activation and VEGF release by bFGF in the T47D breast cancer cell line under normoxic conditions: involvement of PI-3K/Akt and MEK1/ERK pathways[J]. JOURNAL OF PATHOLOGY,2005,205(4):530-536.
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