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学科主题: 临床医学
题名:
Aberrant methylation of the 3q25 tumor suppressor gene PTX3 in human esophageal squamous cell carcinoma
作者: Wang, Jun-Xiong3; He, Yuan-Long2; Zhu, Sheng-Tao1; Yang, Shuo4; Zhang, Shu-Tian1
关键词: Tumor suppressor gene ; Pentraxin 3 ; Microarray ; DNA methylation ; Esophageal squamous cell carcinoma
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2011-10-07
DOI: 10.3748/wjg.v17.i37.4225
卷: 17, 期:37, 页:4225-4230
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: LONG PENTRAXIN PTX3 ; BREAST-CANCER ; EXPRESSION ; INFECTION ; MICE
英文摘要:

AIM: To identify the novel methylation-silenced gene pentraxin 3 (PTX3) in esophageal squamous cell carcinoma (ESCC).

METHODS: PTX3 mRNA expression was examined in six human ESCC cell lines, one human immortalized normal esophageal epithelial cell line, primary ESCC tumor tissue, and paired adjacent nontumor tissue using reverse transcription polymerase chain reaction (RTPCR). Semi-quantitative immunohistochemistry was used to examine cellular localisation and protein levels. Methylation specific PCR and bisulphite genomic sequencing were employed to investigate the methylation of the candidate gene.

RESULTS: In the majority of ESCC cell lines, we found that PTX3 expression was down-regulated due to gene promoter hypermethylation, which was further confirmed by bisulphite genomic sequencing. Demethylation treatment with 5-aza-2′-deoxycytidine restored PTX3 mRNA expression in ESCC cell lines. Methylation was more common in tumor tissues (85%) than in adjacent nontumor tissues (25%) (P < 0.01).

CONCLUSION: PTX3 is down-regulated through promoter hypermethylation in ESCC, and could potentially serve as a biomarker of ESCC. (C) 2011 Baishideng. All rights reserved.

语种: 英语
所属项目编号: 2007AA02Z4Z4 ; 20090460394 ; 7072022
项目资助者: National High Technology Research and Development Program of China (863 Program) ; China Postdoctoral Science Foundation ; Beijing Municipal Natural Science Foundation
WOS记录号: WOS:000297109100011
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59991
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Capital Med Univ, Beijing Friendship Hosp, Dept Gastroenterol, Beijing 100050, Peoples R China
2.Qingdao Municipal Hosp, Dept Gastroenterol, Qingdao 266071, Shandong, Peoples R China
3.Capital Med Univ, Beijing Friendship Hosp, Med & Hlth Ctr, Beijing 100050, Peoples R China
4.Peking Univ Third Hosp, Dept Lab Med, Beijing 100050, Peoples R China

Recommended Citation:
Wang, Jun-Xiong,He, Yuan-Long,Zhu, Sheng-Tao,et al. Aberrant methylation of the 3q25 tumor suppressor gene PTX3 in human esophageal squamous cell carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2011,17(37):4225-4230.
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