IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Aberrant methylation of the 3q25 tumor suppressor gene PTX3 in human esophageal squamous cell carcinoma
Wang, Jun-Xiong3; He, Yuan-Long2; Zhu, Sheng-Tao1; Yang, Shuo4; Zhang, Shu-Tian1
关键词Tumor suppressor gene Pentraxin 3 Microarray DNA methylation Esophageal squamous cell carcinoma
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2011-10-07
DOI10.3748/wjg.v17.i37.4225
17期:37页:4225-4230
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]LONG PENTRAXIN PTX3 ; BREAST-CANCER ; EXPRESSION ; INFECTION ; MICE
英文摘要

AIM: To identify the novel methylation-silenced gene pentraxin 3 (PTX3) in esophageal squamous cell carcinoma (ESCC).

METHODS: PTX3 mRNA expression was examined in six human ESCC cell lines, one human immortalized normal esophageal epithelial cell line, primary ESCC tumor tissue, and paired adjacent nontumor tissue using reverse transcription polymerase chain reaction (RTPCR). Semi-quantitative immunohistochemistry was used to examine cellular localisation and protein levels. Methylation specific PCR and bisulphite genomic sequencing were employed to investigate the methylation of the candidate gene.

RESULTS: In the majority of ESCC cell lines, we found that PTX3 expression was down-regulated due to gene promoter hypermethylation, which was further confirmed by bisulphite genomic sequencing. Demethylation treatment with 5-aza-2′-deoxycytidine restored PTX3 mRNA expression in ESCC cell lines. Methylation was more common in tumor tissues (85%) than in adjacent nontumor tissues (25%) (P < 0.01).

CONCLUSION: PTX3 is down-regulated through promoter hypermethylation in ESCC, and could potentially serve as a biomarker of ESCC. (C) 2011 Baishideng. All rights reserved.

语种英语
WOS记录号WOS:000297109100011
项目编号2007AA02Z4Z4 ; 20090460394 ; 7072022
资助机构National High Technology Research and Development Program of China (863 Program) ; China Postdoctoral Science Foundation ; Beijing Municipal Natural Science Foundation
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59991
专题北京大学第三临床医学院
北京大学第三临床医学院_检验科
作者单位1.Capital Med Univ, Beijing Friendship Hosp, Dept Gastroenterol, Beijing 100050, Peoples R China
2.Qingdao Municipal Hosp, Dept Gastroenterol, Qingdao 266071, Shandong, Peoples R China
3.Capital Med Univ, Beijing Friendship Hosp, Med & Hlth Ctr, Beijing 100050, Peoples R China
4.Peking Univ Third Hosp, Dept Lab Med, Beijing 100050, Peoples R China
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GB/T 7714
Wang, Jun-Xiong,He, Yuan-Long,Zhu, Sheng-Tao,et al. Aberrant methylation of the 3q25 tumor suppressor gene PTX3 in human esophageal squamous cell carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2011,17(37):4225-4230.
APA Wang, Jun-Xiong,He, Yuan-Long,Zhu, Sheng-Tao,Yang, Shuo,&Zhang, Shu-Tian.(2011).Aberrant methylation of the 3q25 tumor suppressor gene PTX3 in human esophageal squamous cell carcinoma.WORLD JOURNAL OF GASTROENTEROLOGY,17(37),4225-4230.
MLA Wang, Jun-Xiong,et al."Aberrant methylation of the 3q25 tumor suppressor gene PTX3 in human esophageal squamous cell carcinoma".WORLD JOURNAL OF GASTROENTEROLOGY 17.37(2011):4225-4230.
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