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学科主题: 口腔医学
题名:
Flow Cytometric Cell Sorting and In Vitro Pre-Osteoinduction Are Not Requirements for In Vivo Bone Formation by Human Adipose-Derived Stromal Cells
作者: Liu, Yunsong1; Zhao, Yan2; Zhang, Xiao1; Chen, Tong1; Zhao, Xianghui1; Ma, Gui-e3; Zhou, Yongsheng1
刊名: PLOS ONE
发表日期: 2013-02-11
DOI: 10.1371/journal.pone.0056002
卷: 8, 期:2
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: MESENCHYMAL STEM-CELLS ; OSTEOGENIC DIFFERENTIATION ; TISSUE REGENERATION ; CALVARIAL DEFECTS ; GROWTH-FACTOR ; SCAFFOLDS ; MARROW ; ENHANCEMENT ; REPAIR ; INDUCTION
英文摘要:

Human adipose-derived stromal cells (hASCs) are a promising cell source for bone tissue engineering. However, before the clinical application of hASCs for the treatment of bone defects, key questions require answers, including whether pre-osteoinduction (OI) and flow cytometric cell purification are indispensible steps for in vivo bone formation by hASCs. In this study, hASCs were purified by flow cytometric cell sorting (FCCS). The osteogenic capabilities of hASCs and purified hASCs with or without pre-osteoinduction were examined through in vitro and in vivo experiments. We found that pre-OI enhanced the in vitro osteogenic capacity of hASCs. However, 8 weeks after in vivo implantation, there were no significant differences between hASCs and hASCs that had undergone OI (hASCs+OI) or between purified hASCs and purified hASCs+OI (P>0.05). Interestingly, we also found that purified hASCs had an osteogenic potential similar to that of unpurified hASCs in vitro and in vivo. These results suggest that FCCS and in vitro pre-OI are not requirements for in vivo bone formation by hASCs.

语种: 英语
所属项目编号: 30901693 ; 81170937 ; BMU20110274
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Ministry of Education
WOS记录号: WOS:000315100000037
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60002
Appears in Collections:北京大学口腔医学院_口腔修复科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Clin Branch 2, Beijing 100871, Peoples R China
3.Chinese Acad Med Sci, Plast Surg Hosp, Body Sculpture Ctr, Beijing 100730, Peoples R China

Recommended Citation:
Liu, Yunsong,Zhao, Yan,Zhang, Xiao,et al. Flow Cytometric Cell Sorting and In Vitro Pre-Osteoinduction Are Not Requirements for In Vivo Bone Formation by Human Adipose-Derived Stromal Cells[J]. PLOS ONE,2013,8(2).
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