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学科主题口腔医学
Flow Cytometric Cell Sorting and In Vitro Pre-Osteoinduction Are Not Requirements for In Vivo Bone Formation by Human Adipose-Derived Stromal Cells
Liu, Yunsong1; Zhao, Yan2; Zhang, Xiao1; Chen, Tong1; Zhao, Xianghui1; Ma, Gui-e3; Zhou, Yongsheng1
刊名PLOS ONE
2013-02-11
DOI10.1371/journal.pone.0056002
8期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Ministry of Education ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Ministry of Education
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]MESENCHYMAL STEM-CELLS ; OSTEOGENIC DIFFERENTIATION ; TISSUE REGENERATION ; CALVARIAL DEFECTS ; GROWTH-FACTOR ; SCAFFOLDS ; MARROW ; ENHANCEMENT ; REPAIR ; INDUCTION
英文摘要

Human adipose-derived stromal cells (hASCs) are a promising cell source for bone tissue engineering. However, before the clinical application of hASCs for the treatment of bone defects, key questions require answers, including whether pre-osteoinduction (OI) and flow cytometric cell purification are indispensible steps for in vivo bone formation by hASCs. In this study, hASCs were purified by flow cytometric cell sorting (FCCS). The osteogenic capabilities of hASCs and purified hASCs with or without pre-osteoinduction were examined through in vitro and in vivo experiments. We found that pre-OI enhanced the in vitro osteogenic capacity of hASCs. However, 8 weeks after in vivo implantation, there were no significant differences between hASCs and hASCs that had undergone OI (hASCs+OI) or between purified hASCs and purified hASCs+OI (P>0.05). Interestingly, we also found that purified hASCs had an osteogenic potential similar to that of unpurified hASCs in vitro and in vivo. These results suggest that FCCS and in vitro pre-OI are not requirements for in vivo bone formation by hASCs.

语种英语
所属项目编号30901693 ; 81170937 ; BMU20110274
资助者National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Ministry of Education ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Ministry of Education
WOS记录号WOS:000315100000037
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60002
专题北京大学口腔医学院_口腔修复科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Clin Branch 2, Beijing 100871, Peoples R China
3.Chinese Acad Med Sci, Plast Surg Hosp, Body Sculpture Ctr, Beijing 100730, Peoples R China
推荐引用方式
GB/T 7714
Liu, Yunsong,Zhao, Yan,Zhang, Xiao,et al. Flow Cytometric Cell Sorting and In Vitro Pre-Osteoinduction Are Not Requirements for In Vivo Bone Formation by Human Adipose-Derived Stromal Cells[J]. PLOS ONE,2013,8(2).
APA Liu, Yunsong.,Zhao, Yan.,Zhang, Xiao.,Chen, Tong.,Zhao, Xianghui.,...&Zhou, Yongsheng.(2013).Flow Cytometric Cell Sorting and In Vitro Pre-Osteoinduction Are Not Requirements for In Vivo Bone Formation by Human Adipose-Derived Stromal Cells.PLOS ONE,8(2).
MLA Liu, Yunsong,et al."Flow Cytometric Cell Sorting and In Vitro Pre-Osteoinduction Are Not Requirements for In Vivo Bone Formation by Human Adipose-Derived Stromal Cells".PLOS ONE 8.2(2013).
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