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N-cadherin expression in a rat model of retinal detachment and reattachment
Chen, Hui-Jin; Ma, Zhi-Zhong
刊名INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2007-04-01
DOI10.1167/iovs.06-0928
48期:4页:1832-1838
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]CELL-ADHESION MOLECULES ; EXPERIMENTAL PROLIFERATIVE VITREORETINOPATHY ; PIGMENT EPITHELIAL-CELLS ; GROWTH-FACTOR RECEPTOR ; NEURAL RETINA ; RISK-FACTORS ; IN-VITRO ; PHENOTYPE ; MODULATION ; INTEGRINS
英文摘要

PURPOSE. To observe the changes in N-cadherin expression in the retina after experimental retinal detachment (RD) and reattachment in the rat and to explore the role N-cadherin might play after RD.

METHODS. Forty rat retinas were detached by transscleral injection of 1.4% sodium hyaluronate into the subretinal space. The eyes were enucleated at different time intervals (n = 5), followed by fixation, embedding, and sectioning. The differences in N-cadherin expression in the normal retina, detached retina, and spontaneously reattached retina were determined. Furthermore, an N-cadherin antagonist was injected in combination with 1.4% sodium hyaluronate into the subretinal space in another 10 eyes, in an attempt to demonstrate the role N-cadherin plays after RD.

RESULTS. N-cadherin was not expressed in the RPE layer of the normal rat retina. After RD, intense immunolabeling of N-cadherin was seen in the RPE cells, the photoreceptors, and the outer limiting membrane (OLM). An increasing number of cytokeratin (CK)-positive cells likely to be RPE cells was found attached to the outer surface of the detached neural retina. Where the retina was reattached, the N-cadherin immunolabeling rapidly decreased. In eyes treated with an N-cadherin antagonist, the retinas appeared thinner than that in eyes without treatment, and the photoreceptor nuclei showed significantly loss. Moreover, CK-positive cells attached to the outer surface of the detached retina were markedly fewer in number.

CONCLUSIONS. Increased expression of N-cadherin in the RPE cells, the photoreceptor cells, and the OLM of the retina after RD may contribute to RPE cell migration and photoreceptor survival. These changes could be reversed by retinal reattachment.

语种英语
WOS记录号WOS:000245408200051
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60003
专题北京大学第三临床医学院_眼科
作者单位Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100083, Peoples R China
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Chen, Hui-Jin,Ma, Zhi-Zhong. N-cadherin expression in a rat model of retinal detachment and reattachment[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2007,48(4):1832-1838.
APA Chen, Hui-Jin,&Ma, Zhi-Zhong.(2007).N-cadherin expression in a rat model of retinal detachment and reattachment.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,48(4),1832-1838.
MLA Chen, Hui-Jin,et al."N-cadherin expression in a rat model of retinal detachment and reattachment".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 48.4(2007):1832-1838.
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