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Early-life epileptiform discharges exert both rapid and long-lasting effects on AMPAR subunit composition and distribution in developing neurons
Jiang, Qian; Wang, Jingmin; Wu, Ye; Wu, Xiru; Qin, Jiong; Jiang, Yuwu
关键词seizure-like insults development cortical neurons long-lasting AMPAR subunits sub-cellular fractionation
刊名NEUROSCIENCE LETTERS
2008-10-17
DOI10.1016/j.neulet.2008.08.006
444期:1页:31-35
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]GLUTAMATE-RECEPTOR ; SYNAPTIC PLASTICITY ; CORTICAL-NEURONS ; SEIZURE SUSCEPTIBILITY ; PERINATAL SEIZURES ; IMMATURE BRAIN ; NMDA RECEPTOR ; TRAFFICKING ; HIPPOCAMPAL ; EXPRESSION
英文摘要

The perinatal period of brain is characterized by dynamic changes in structure and high propensity for epilepsy. Animal models have shown that alterations of AMPA receptor (AMPAR) assembly or function may be related to seizure-induced cell damage, long-lasting impairments in brain development and seizure threshold. However, effects of earlier epileptiform discharges on AMPAR composition and sub-cellular distribution remain understudied. In this study, we analyzed age-dependent variation of relative GluR1 and GluR2 protein levels in primary cultured rat cortical neurons at 7 DIV, 12 DIV, 17 DIV and 21 DIV. By inducing a single event of epileptiform activity at 6 DIV, we tested the effects of early-life seizure-like insults on AMPAR subunit distribution. We found a significant increase in synaptosomal membrane GluR1 expression in magnesium-free (MGF) medium-treated neurons at each time point detected (p < 0.05), while GluR2 expression increased at 7 DIV, and declined at 17 DIV and 21 DIV respectively (p < 0.05). That is, a trend of high GluR1 with much lower GluR2 expression on the surface membrane of epileptiform discharges experienced neurons over time in culture was presented. These findings in an in vitro model of early-life seizure may inform rodent models of epilepsy, as well as the cellular mechanism involved in epilepsy-associated brain dysfunction. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000260066900007
项目编号30270448 ; 30470555
资助机构National Natural Science Foundation of China
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60028
专题北京大学第一临床医学院_儿科
北京大学第二临床医学院_儿科
作者单位Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
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GB/T 7714
Jiang, Qian,Wang, Jingmin,Wu, Ye,et al. Early-life epileptiform discharges exert both rapid and long-lasting effects on AMPAR subunit composition and distribution in developing neurons[J]. NEUROSCIENCE LETTERS,2008,444(1):31-35.
APA Jiang, Qian,Wang, Jingmin,Wu, Ye,Wu, Xiru,Qin, Jiong,&Jiang, Yuwu.(2008).Early-life epileptiform discharges exert both rapid and long-lasting effects on AMPAR subunit composition and distribution in developing neurons.NEUROSCIENCE LETTERS,444(1),31-35.
MLA Jiang, Qian,et al."Early-life epileptiform discharges exert both rapid and long-lasting effects on AMPAR subunit composition and distribution in developing neurons".NEUROSCIENCE LETTERS 444.1(2008):31-35.
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