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学科主题: 临床医学
题名:
Early-life epileptiform discharges exert both rapid and long-lasting effects on AMPAR subunit composition and distribution in developing neurons
作者: Jiang, Qian; Wang, Jingmin; Wu, Ye; Wu, Xiru; Qin, Jiong; Jiang, Yuwu
关键词: seizure-like insults ; development ; cortical neurons ; long-lasting ; AMPAR subunits ; sub-cellular fractionation
刊名: NEUROSCIENCE LETTERS
发表日期: 2008-10-17
DOI: 10.1016/j.neulet.2008.08.006
卷: 444, 期:1, 页:31-35
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: GLUTAMATE-RECEPTOR ; SYNAPTIC PLASTICITY ; CORTICAL-NEURONS ; SEIZURE SUSCEPTIBILITY ; PERINATAL SEIZURES ; IMMATURE BRAIN ; NMDA RECEPTOR ; TRAFFICKING ; HIPPOCAMPAL ; EXPRESSION
英文摘要:

The perinatal period of brain is characterized by dynamic changes in structure and high propensity for epilepsy. Animal models have shown that alterations of AMPA receptor (AMPAR) assembly or function may be related to seizure-induced cell damage, long-lasting impairments in brain development and seizure threshold. However, effects of earlier epileptiform discharges on AMPAR composition and sub-cellular distribution remain understudied. In this study, we analyzed age-dependent variation of relative GluR1 and GluR2 protein levels in primary cultured rat cortical neurons at 7 DIV, 12 DIV, 17 DIV and 21 DIV. By inducing a single event of epileptiform activity at 6 DIV, we tested the effects of early-life seizure-like insults on AMPAR subunit distribution. We found a significant increase in synaptosomal membrane GluR1 expression in magnesium-free (MGF) medium-treated neurons at each time point detected (p < 0.05), while GluR2 expression increased at 7 DIV, and declined at 17 DIV and 21 DIV respectively (p < 0.05). That is, a trend of high GluR1 with much lower GluR2 expression on the surface membrane of epileptiform discharges experienced neurons over time in culture was presented. These findings in an in vitro model of early-life seizure may inform rodent models of epilepsy, as well as the cellular mechanism involved in epilepsy-associated brain dysfunction. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 30270448 ; 30470555
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000260066900007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60028
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Jiang, Qian,Wang, Jingmin,Wu, Ye,et al. Early-life epileptiform discharges exert both rapid and long-lasting effects on AMPAR subunit composition and distribution in developing neurons[J]. NEUROSCIENCE LETTERS,2008,444(1):31-35.
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