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学科主题: 基础医学
题名:
Development of multiplex nucleic acid sequence-based amplification for detection of human respiratory tract viruses
作者: Lau, Lok Ting1,2,3,4,5,6,7; Feng, Xiao Yan1,2,3,4,5; Lam, Tsz Yan5,7; Hui, Hung Kit5,7; Yu, Albert Cheung Hoi1,2,3,4,5,6,7
关键词: Nucleic acid sequence-based amplification ; Enzyme-linked oligonucleotide capture ; Multiplex ; Influenza ; Enzymes-linked immunosorbent assay
刊名: JOURNAL OF VIROLOGICAL METHODS
发表日期: 2010-09-01
DOI: 10.1016/j.jviromet.2010.04.027
卷: 168, 期:1-2, 页:251-254
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
关键词[WOS]: MOUTH-DISEASE VIRUS ; SYNCYTIAL-VIRUS ; INFLUENZA-A ; B VIRUSES ; PCR ; INFECTIONS ; NASBA
英文摘要:

A group of common lower respiratory tract infections, influenza A, influenza B, human parainfluenza virus 1-4 (HPIV1-4), respiratory syncytial virus (RSV), rubella virus (RV) and Coxsackie virus (CSV), were selected for the development of a multiplex nucleic acid sequence-based amplification (NASBA) assay. Quantifiable measurement utilizing an enzyme-linked oligonucleotide capture (EOC) optical detection method, which was described previously, alleviated the requirement of specialized instrumentation that is commonly used in other molecular techniques. Multiplex NASBA-EOC provided rapid and specific detection of a single virus from a multiplexed group, reducing laboratory testing time and enabling high throughput screening. The uniquely designed primers and probes proved to be highly sensitive and specific, exemplifying the robustness of the multiplex NASBA-EOC technique. (C) 2010 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2006AA02Z452 ; 30870818 ; 30670644 ; 7091004
项目资助者: National High Technology Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; Beijing National Natural Science Foundation of China
WOS记录号: WOS:000280869400042
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60049
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Neurobiol, Beijing 100191, Peoples R China
3.Peking Univ, Ctr Infect Dis, Beijing 100191, Peoples R China
4.Hai Kang Life Corp Ltd, Shau Kei Wan, Hong Kong, Peoples R China
5.Peking Univ, Key Lab Neurosci, Minist Educ, Beijing 100191, Peoples R China
6.Peking Univ, Key Lab Neurosci, Minist Publ Hlth, Beijing 100191, Peoples R China
7.Beijing Hai Kang DNA Chips Ltd, Beijing Econ Dev Area, Beijing, Peoples R China

Recommended Citation:
Lau, Lok Ting,Feng, Xiao Yan,Lam, Tsz Yan,et al. Development of multiplex nucleic acid sequence-based amplification for detection of human respiratory tract viruses[J]. JOURNAL OF VIROLOGICAL METHODS,2010,168(1-2):251-254.
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