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学科主题: 临床医学
题名:
Role of NF-kappa B and MAPKs in light-induced photoreceptor apoptosis
作者: Yang, Li-ping; Zhu, Xiu-an; Tso, Mark O. M.
刊名: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
发表日期: 2007-10-01
DOI: 10.1167/iovs.06-0871
卷: 48, 期:10, 页:4766-4776
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: ACTIVATED PROTEIN-KINASE ; INDUCED RETINAL DEGENERATION ; TUMOR-NECROSIS-FACTOR ; NITRIC-OXIDE ; PIGMENT REGENERATION ; EPITHELIAL-CELLS ; BINDING PROTEIN ; TRANSGENIC MICE ; MICROGLIA ; MINOCYCLINE
英文摘要:

PURPOSE. To elucidate the role of nuclear factor kappa B (NF-kappa B) and mitogen-activated protein kinases (MAPKs) in light-induced apoptosis of photoreceptors in culture and to explore the potential inhibitory effect of minocycline and sulforaphane on apoptosis.

METHODS. Apoptosis of 661W cells was induced by exposure to light and was detected by terminal dUTP transferase nick end labeling ( TUNEL). The mRNA expression and protein production of 10 chemokines and noxious factors were examined by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay ( ELISA). The protein expression of the p65 subunit of NF-kappa B, and the MAPKs p-p38, p-p44/42, and p-JNK were examined by Western blot and immunofluorescence analyses.

RESULTS. After exposure to light for 4 hours, 60% to 70% of the 661W cells underwent apoptosis. The expression of five selected chemokines and noxious factors was upregulated. The protein expression of the p65 subunit of NF-kappa B was downregulated, and the expression of the MAPKs p-p38, p-p44/42, and p-JNK was upregulated. Pretreatment with SB203580 for 1 hour inhibited light-induced upregulation of p-p38 and inhibited photoreceptor apoptosis. Pretreatment with minocycline or sulforaphane for 1 hour inhibited light-induced downregulation of the NF-kappa B p65 subunit and inhibited photoreceptor apoptosis.

CONCLUSIONS. Apoptotic photoreceptors secrete chemokines and noxious factors to induce an immunologic response. The NF-kappa B and MAPK pathways both are involved in light-induced 661W photoreceptor apoptosis. Minocycline and sulforaphane inhibit light-induced photoreceptor apoptosis, partly through an NF-kappa B-dependent mechanism, but not through the MAPK pathway.

语种: 英语
WOS记录号: WOS:000249757600050
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60068
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Ho !E 871, Peoples R China
2.Johns Hopkins Univ, Wilmer Eye Inst, Sch Med, Baltimore, MD 21287 USA

Recommended Citation:
Yang, Li-ping,Zhu, Xiu-an,Tso, Mark O. M.. Role of NF-kappa B and MAPKs in light-induced photoreceptor apoptosis[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2007,48(10):4766-4776.
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