|Effects of chemokine-like factor 1 on vascular smooth muscle cell migration and proliferation in vascular inflammation|
|Zhang, Tao1; Zhang, Xiaoming1; Yu, Weidong2; Chen, Jian3; Li, Qingle1; Jiao, Yang1; He, Peiying2; Shen, Chenyang1|
|关键词||Chemokine-like Factor 1 Vascular Smooth Muscle Cell Migration And Proliferation Neointima Formation Atherosclerosis|
|WOS标题词||Science & Technology|
|类目[WOS]||Peripheral Vascular Disease|
|研究领域[WOS]||Cardiovascular System & Cardiology|
|关键词[WOS]||INTIMAL HYPERPLASIA ; LOWER-EXTREMITY ; DISEASE ; MECHANISMS ; ATHEROSCLEROSIS ; DYSFUNCTION ; EXPRESSION ; CYTOKINES ; ANTISENSE ; SURGERY|
Objective: Vascular smooth muscle cell (VSMC) migration and proliferation are key components of vascular inflammation that may lead to atherosclerosis and restenosis, in which cytokines are considered as pivotal factors regarding recruitment of VSMC. A member of recently described family of chemokines, chemokine-like factor 1 (CKLF1), displays a wide spectrum of chemotaxis. This study investigated the role of CKLF1 in VSMC migration and proliferation during the process of vascular inflammation.
Methods and results: : The effects of CKLF1 on migration, proliferation and neointimal formation were investigated in cultured VSMCs, rat balloon injured arteries and human atherosclerotic plaques. CKLF1 overexpression greatly enhanced, whereas shRNA knockdown markedly retarded, VSMC migration and proliferation in vitro. In addition, CKLF1 protein accumulated preferentially in neointima of the injured rat arteries in vivo. CKLF1 overexpression resulted in a 2.5-fold increase in intimal thickness. In contrast, shRNA-mediated CKLF1 knockdown significantly suppressed neointima formation by 70% compared that in control group. Intriguingly, besides animal model, higher level of CKLF1 was observed in human atherosclerotic plaques than that in normal arteries.
Conclusion: CKLF1 plays an essential role in migration and proliferation of VSMCs, which in turn facilitated neointimal hyperplasia and atherosclerosis. Inhibition of CKLF1 activity provides a potential target for the prevention of atherosclerosis and restenosis. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
|资助机构||National Natural Science Foundation of the People&prime ; s Republic of China|
|作者单位||1.Peking Univ, Peoples Hosp, Dept Vasc Surg, Beijing 100044, Peoples R China|
2.Peking Univ, Peoples Hosp, Dept Cent Lab, Beijing 100044, Peoples R China
3.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100190, Peoples R China
|Zhang, Tao,Zhang, Xiaoming,Yu, Weidong,et al. Effects of chemokine-like factor 1 on vascular smooth muscle cell migration and proliferation in vascular inflammation[J]. ATHEROSCLEROSIS,2013,226(1):49-57.|
|APA||Zhang, Tao.,Zhang, Xiaoming.,Yu, Weidong.,Chen, Jian.,Li, Qingle.,...&Shen, Chenyang.(2013).Effects of chemokine-like factor 1 on vascular smooth muscle cell migration and proliferation in vascular inflammation.ATHEROSCLEROSIS,226(1),49-57.|
|MLA||Zhang, Tao,et al."Effects of chemokine-like factor 1 on vascular smooth muscle cell migration and proliferation in vascular inflammation".ATHEROSCLEROSIS 226.1(2013):49-57.|