|Intraocular expression of thymosin beta 4 in proliferative diabetic retinopathy|
|Wang, Jia-Yan1; Lu, Qiang1; Tao, Yong1,2; Jiang, Yan-Rong1; Jonas, Jost B.2|
|关键词||diabetes mellitus diabetic retinopathy neovascularization thymosin beta 4|
|WOS标题词||Science & Technology|
|关键词[WOS]||ENDOTHELIAL GROWTH-FACTOR ; MACULAR DEGENERATION ; VISUAL IMPAIRMENT ; CELLS ; ERYTHROPOIETIN ; FLUID ; ANGIOGENESIS ; TISSUES ; PROTEIN ; EDEMA|
Purpose: To examine an association between thymosin beta 4 as potentially angio-proliferative factor and proliferative diabetic retinopathy.
Methods: The clinical study part included 62 patients with proliferative diabetic retinopathy (PDR) (study group) and 24 patients with non-diabetic pre-retinal membranes (control group). All patients underwent pars plana vitrectomy. We examined the thymosin beta 4 concentration in vitreous and plasma; and the expression of thymosin beta 4, glial fibrillary acidic protein (GFAP) and CD31 (PECAM-1 or Platelet Endothelial Cell Adhesion Molecule) and the levels of thymosin beta 4 mRNA and vascular endothelial growth factor (VEGF) mRNA in the excised membranes. The experimental study part consisted of 24 Sprague-Dawley rats with streptozotocin-induced diabetes mellitus and 24 age-matched control animals without diabetes. We determined the mRNA concentrations of thymosin beta 4, VEGF and GFAP in the rat retinas.
Results: In the clinical study part, the vitreal and plasma thymosin beta 4 concentrations were significantly higher in the study group than control group (p = 0.04 and p = 0.01, respectively), and were significantly (p = 0.028) correlated with each other. Co-expression of thymosin beta 4 and CD31 was observed in the diabetic fibrovascular membranes. Thymosin beta 4 mRNA and VEGF mRNA levels were significantly (p < 0.01) higher in diabetic membranes than in non-diabetic membranes. In the experimental study part, the diabetic retinas showed co-localization of thymosin beta 4 and GFAP. The mRNA levels of thymosin b4, VEGF and GFAP were significantly (p < 0.01) higher in diabetic rats than in control animals.
Conclusions: Thymosin beta 4 was produced in intraocular fibrovascular membranes of patients with PDR and in rats with experimental diabetes mellitus. Thymosin beta 4 may play a role in diabetic retinal neovascularization.
|作者单位||1.Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing 100044, Peoples R China|
2.Heidelberg Univ, Med Fac Mannheim, Dept Ophthalmol, Heidelberg, Germany
|Wang, Jia-Yan,Lu, Qiang,Tao, Yong,et al. Intraocular expression of thymosin beta 4 in proliferative diabetic retinopathy[J]. ACTA OPHTHALMOLOGICA,2011,89(5):E396-E403.|
|APA||Wang, Jia-Yan,Lu, Qiang,Tao, Yong,Jiang, Yan-Rong,&Jonas, Jost B..(2011).Intraocular expression of thymosin beta 4 in proliferative diabetic retinopathy.ACTA OPHTHALMOLOGICA,89(5),E396-E403.|
|MLA||Wang, Jia-Yan,et al."Intraocular expression of thymosin beta 4 in proliferative diabetic retinopathy".ACTA OPHTHALMOLOGICA 89.5(2011):E396-E403.|