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A clinical comparative study of polymerase chain reaction assay for diagnosis of pneumocystis pneumonia in non-AIDS patients
Mu Xiang-dong; Wang Guang-fa; Su Li
关键词Pneumocystis Jirovecii Pneumonia Sputum Bronchioalveolar Lavage Fluid Gomori Methenamine Silver Stains Polymerase Chain Reaction
刊名CHINESE MEDICAL JOURNAL
2011-09-05
DOI10.3760/cma.j.issn.0366-6999.2011.17.021
124期:17页:2683-2686
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
资助者Peking University First Hospital Research Foundation ; Peking University First Hospital Research Foundation
研究领域[WOS]General & Internal Medicine
关键词[WOS]BRONCHOALVEOLAR LAVAGE SPECIMENS ; REAL-TIME PCR ; CARINII-PNEUMONIA ; JIROVECI PNEUMONIA ; INFECTED PATIENTS ; INDUCED SPUTUM
英文摘要

Background Pneumocystis jirovecii pneumonia (PCP) is one of the most common and fatal infections in non-AIDS immunocompromised patients, which is difficult to diagnose by traditional morphologic methods. This study evaluated polymerase chain reaction (PCR) assays of Pneumocystis jirovecii mitochondrial large subunits ribosomal RNA in sputum and bronchioalveolar lavage fluid (BALF) for diagnosing PCP.

Methods Sputum and BALF specimens from two groups were collected: one group (PCP group) included 20 patients definitely diagnosed of PCP by Gomori methenamine silver (GMS) stains of BALF; the other group (non-PCP group) included 40 patients. Each specimen was examined by GMS stains and PCR assays.

Results GMS stains of BALF in PCP group were 100% positive (20/20), GMS stains of sputum in PCP group were 35% positive (7/20); GMS stains of BALF in non-PCP group were 100% negative (40/40), GMS stains of sputum in non-PCP group were 100% negative (40/40). PCR assays of BALF in PCP group were 100% positive (20/20), PCR assays of sputum in PCP group were 100% positive (20/20); PCR assays of BALF in non-PCP group were 100% negative (40/40), PCR assays of sputum in non-PCP group were 100% negative (40/40). Sensitivity and specificity of PCR assays of sputum and BALF were both 100%; positive and negative predictive values were also both 100%.

Conclusion The diagnostic value of PCR assays of Pneumocystis jirovecii mitochondrial large subunits ribosomal RNA on sputum and BALF for pneumocystis pneumonia are both high and equivalent. Chin Med J 2011;124(17):2683-2686

语种英语
资助者Peking University First Hospital Research Foundation ; Peking University First Hospital Research Foundation
WOS记录号WOS:000295297400021
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60109
Collection北京大学第一临床医学院_呼吸和危重症医学科
作者单位Peking Univ, Dept Resp Med, Hosp 1, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Mu Xiang-dong,Wang Guang-fa,Su Li. A clinical comparative study of polymerase chain reaction assay for diagnosis of pneumocystis pneumonia in non-AIDS patients[J]. CHINESE MEDICAL JOURNAL,2011,124(17):2683-2686.
APA Mu Xiang-dong,Wang Guang-fa,&Su Li.(2011).A clinical comparative study of polymerase chain reaction assay for diagnosis of pneumocystis pneumonia in non-AIDS patients.CHINESE MEDICAL JOURNAL,124(17),2683-2686.
MLA Mu Xiang-dong,et al."A clinical comparative study of polymerase chain reaction assay for diagnosis of pneumocystis pneumonia in non-AIDS patients".CHINESE MEDICAL JOURNAL 124.17(2011):2683-2686.
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