|Anti-tumor Effect of Integrin Targeted Lu-177-3PRGD(2) and Combined Therapy with Endostar|
|Shi, Jiyun1,2; Fan, Di1,3; Dong, Chengyan1,3; Liu, Hao1,3; Jia, Bing1,3; Zhao, Huiyun1,2; Jin, Xiaona4; Liu, Zhaofei1,3; Li, Fang4; Wang, Fan1,3|
|关键词||Integrin alpha(v)beta(3) Arg-Gly-Asp (RGD) Lu-177 radionuclide therapy combination therapy|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, Research & Experimental|
|研究领域[WOS]||Research & Experimental Medicine|
|关键词[WOS]||IMPROVING TUMOR UPTAKE ; RGD PEPTIDES ; NEUROENDOCRINE TUMORS ; CANCER ; RADIOTHERAPY ; EXPRESSION ; EFFICACY ; MODEL ; RADIOIMMUNOTHERAPY ; CHEMORADIOTHERAPY|
Purpose: Targeted radiotherapy (TRT) is an emerging approach for tumor treatment. Previously, 3PRGD(2) (a dimeric RGD peptide with 3 PEG(4) linkers) has been demonstrated to be of advantage for integrin alpha(v)beta(3) targeting. Given the promising results of Tc-99m-3PRGD(2) for lung cancer detection in human beings, we are encouraged to investigate the radiotherapeutic efficacy of radiolabeled 3PRGD(2). The goal of this study was to investigate and optimize the integrin alpha(v)beta(3) mediated therapeutic effect of Lu-177-3PRGD(2) in the animal model.
Experimental Design: Biodistribution, gamma imaging and maximum tolerated dose (MTD) studies of Lu-177-3PRGD(2) were performed. The targeted radiotherapy (TRT) with single dose and repeated doses as well as the combined therapy of TRT and the anti-angiogenic therapy (AAT) with Endostar were conducted in U87MG tumor model. The hematoxylin and eosin (H&E) staining and immunochemistry (IHC) were performed post-treatment to evaluate the therapeutic effect.
Results: The U87MG tumor uptake of Lu-177-3PRGD(2) was relatively high (6.03 +/- 0.65 % ID/g, 4.62 +/- 1.44 % ID/g, 3.55 +/- 1.08 % ID/g, and 1.22 +/- 0.18 % ID/g at 1 h, 4 h, 24 h, and 72 h postinjection, respectively), and the gamma imaging could visualize the tumors clearly. The MTD of Lu-177-3PRGD(2) in nude mice (> 111 MBq) was twice to that of Y-90-3PRGD(2) (55.5 MBq). U87MG tumor growth was significantly delayed by Lu-177-3PRGD(2) TRT. Significantly increased anti-tumor effects were observed in the two doses or combined treatment groups.
Conclusion: The two-dose TRT and combined therapy with Endostar potently enhanced the tumor growth inhibition, but the former does not need to inject daily for weeks, avoiding a lot of unnecessary inconvenience and suffering for patients, which could potentially be rapidly translated into clinical practice in the future.
|项目编号||81125011 ; 2013CB733802 ; 2011CB707703 ; 81000625 ; 30930030 ; 81222019 ; 30900373 ; 81201127 ; 81028009 ; 81321003 ; 2012ZX09102301-018 ; 2011YQ030114 ; 2012BAK25B03-16 ; 31300 ; BMU20110263|
|资助机构||Outstanding Youth Fund ; "973" program ; National Natural Science Foundation of China (NSFC) ; Ministry of Science and Technology of China ; Ministry of Education of China|
|作者单位||1.Peking Univ, Dept Radiat Med, Beijing 100191, Peoples R China|
2.Peking Univ, Med Isotopes Res Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Med & Healthy Analyt Ctr, Beijing 100191, Peoples R China
4.Beijing Union Med Coll Hosp, Dept Nucl Med, Beijing 100857, Peoples R China
|Shi, Jiyun,Fan, Di,Dong, Chengyan,et al. Anti-tumor Effect of Integrin Targeted Lu-177-3PRGD(2) and Combined Therapy with Endostar[J]. THERANOSTICS,2014,4(3):256-266.|
|APA||Shi, Jiyun.,Fan, Di.,Dong, Chengyan.,Liu, Hao.,Jia, Bing.,...&Wang, Fan.(2014).Anti-tumor Effect of Integrin Targeted Lu-177-3PRGD(2) and Combined Therapy with Endostar.THERANOSTICS,4(3),256-266.|
|MLA||Shi, Jiyun,et al."Anti-tumor Effect of Integrin Targeted Lu-177-3PRGD(2) and Combined Therapy with Endostar".THERANOSTICS 4.3(2014):256-266.|