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Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression
Bai, Yang1,4; Yang, Weiwei1,4; Yang, Hui-xia2; Liao, Qinping2; Ye, Gang3; Fu, Guodong3; Ji, Lei1; Xu, Peng1,4; Wang, Hao1,4; Li, Yu-xia1; Peng, Chun3; Wang, Yan-ling1
刊名PLOS ONE
2012-06-19
DOI10.1371/journal.pone.0038875
7期:6
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Chinese National Special Fund for Basic Research Project ; National Natural Sciences Foundation ; Canadian Institutes of Health Research ; Chinese National Special Fund for Basic Research Project ; National Natural Sciences Foundation ; Canadian Institutes of Health Research
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]SUPPRESSES TUMORIGENICITY ; HEPATOCELLULAR-CARCINOMA ; PROMOTES APOPTOSIS ; SIGNALING PATHWAYS ; ACTIVIN RECEPTORS ; MICRORNA TARGETS ; MESSENGER-RNAS ; PROLIFERATION ; DIFFERENTIATION ; DISEASES
英文摘要

Background: Preeclampsia (PE) is a pregnancy-specific syndrome manifested by on-set of hypertension and proteinuria after 20 weeks of gestation. Abnormal placenta development has been generally accepted as initial cause of the disorder. Recently, miR-195 was found to be down-regulated in preeclamptic placentas compared with normal pregnant ones, indicating possible association of this small molecule with placental pathology of preeclampsia. By far the function of miR-195 in the development of placenta remains unknown.

Methodology/Principal Findings: Bioinformatic assay predicted ActRIIA as one of the targets for miR-195. By using Real-time PCR, Western blotting and Dual Luciferase Assay, we validated that ActRIIA was the direct target of miR-195 in human trophoblast cells. Transwell insert invasion assay showed that miR-195 could promote cell invasion in trophoblast cell line, HTR8/SVneo cells, and the effect could be abrogated by overexpressed ActRIIA. In preeclamptic placenta tissues, pri-miR-195 and mature miR-195 expressions were down-regulated, whereas ActRIIA level appeared to be increased when compared with that in gestational-week-matched normal placentas.

Conclusions/Significance: This is the first report on the function of miR-195 in human placental trophoblast cells which reveals an invasion-promoting effect of the small RNA via repressing ActRIIA. Aberrant expression of miR-195 may contribute to the occurrence of preeclampsia through interfering with Activin/Nodal signaling mediated by ActRIIA in human placenta.

语种英语
所属项目编号2011CB944400 ; 81025004 ; 3081120430 ; CCI-92222 ; MOP-81370
资助者Chinese National Special Fund for Basic Research Project ; National Natural Sciences Foundation ; Canadian Institutes of Health Research ; Chinese National Special Fund for Basic Research Project ; National Natural Sciences Foundation ; Canadian Institutes of Health Research
WOS记录号WOS:000305652700035
引用统计
被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60115
专题北京大学第一临床医学院
作者单位1.York Univ, Dept Biol, Toronto, ON M3J 2R7, Canada
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
4.Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Bai, Yang,Yang, Weiwei,Yang, Hui-xia,et al. Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression[J]. PLOS ONE,2012,7(6).
APA Bai, Yang.,Yang, Weiwei.,Yang, Hui-xia.,Liao, Qinping.,Ye, Gang.,...&Wang, Yan-ling.(2012).Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression.PLOS ONE,7(6).
MLA Bai, Yang,et al."Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression".PLOS ONE 7.6(2012).
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