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学科主题: 临床医学
题名:
Connective tissue growth factor stimulates renal cortical myofibroblast-like cell proliferation and matrix protein production
作者: Gao, Xuxia1,2; Li, Jingzi1,2; Huang, Haichang1,2; Li, Xiaomei1,2
刊名: WOUND REPAIR AND REGENERATION
发表日期: 2008-05-01
DOI: 10.1111/j.1524-475X.2008.00380.x
卷: 16, 期:3, 页:408-415
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Dermatology ; Medicine, Research & Experimental ; Surgery
研究领域[WOS]: Cell Biology ; Dermatology ; Research & Experimental Medicine ; Surgery
关键词[WOS]: HUMAN MESANGIAL CELLS ; TUBULOINTERSTITIAL FIBROSIS ; IN-VITRO ; FIBROBLAST PROLIFERATION ; INTERSTITIAL FIBROSIS ; INJURY ; DIFFERENTIATION ; EXPRESSION ; ACTIVATION ; KIDNEY
英文摘要:

Myofibroblasts primarily contribute to the pathogenesis of renal interstitial fibrosis by unregulated cell proliferation and synthesis of excessive amounts of extracellular matrix (ECM) proteins. We used cultured myofibroblast-like cells obtained by outgrowth from explants of rat kidney cortex to study the effects and relevant signaling pathway of connective tissue growth factor (CTGF) on cell proliferation and ECM production. Exogenous CTGF stimulated proliferation of myofibroblast-like cells in a dose- and time-dependent manner. CTGF also increased the secretion of fibronectin and collagen I protein in the supernatant medium. Nevertheless, CTGF did not affect matrix-degrading metalloproteinases-2 and -9 activities in supernatant medium measured by gelatin zymography. CTGF induced activation of extracellular signal-regulated protein kinase (ERK)1/2 mitogen-activated protein kinase pathway as early as 5 minutes. Inhibition of ERK1/2 activation with PD98059 completely blocked CTGF-induced cell proliferation as well as secretion of fibronectin and collagen I protein. The above results indicate that CTGF triggers cell proliferation and production of ECM proteins in cultured myofibroblast-like cells through the ERK1/2 mitogen-activated protein kinase pathway.

语种: 英语
WOS记录号: WOS:000255666500011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60122
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: 1.Peking Univ, Inst Nephrol, Beijing 100034, Peoples R China
2.Peking Univ, Div Nephrol, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Gao, Xuxia,Li, Jingzi,Huang, Haichang,et al. Connective tissue growth factor stimulates renal cortical myofibroblast-like cell proliferation and matrix protein production[J]. WOUND REPAIR AND REGENERATION,2008,16(3):408-415.
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