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学科主题临床医学
Connective tissue growth factor stimulates renal cortical myofibroblast-like cell proliferation and matrix protein production
Gao, Xuxia1,2; Li, Jingzi1,2; Huang, Haichang1,2; Li, Xiaomei1,2
刊名WOUND REPAIR AND REGENERATION
2008-05-01
DOI10.1111/j.1524-475X.2008.00380.x
16期:3页:408-415
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Dermatology ; Medicine, Research & Experimental ; Surgery
研究领域[WOS]Cell Biology ; Dermatology ; Research & Experimental Medicine ; Surgery
关键词[WOS]HUMAN MESANGIAL CELLS ; TUBULOINTERSTITIAL FIBROSIS ; IN-VITRO ; FIBROBLAST PROLIFERATION ; INTERSTITIAL FIBROSIS ; INJURY ; DIFFERENTIATION ; EXPRESSION ; ACTIVATION ; KIDNEY
英文摘要

Myofibroblasts primarily contribute to the pathogenesis of renal interstitial fibrosis by unregulated cell proliferation and synthesis of excessive amounts of extracellular matrix (ECM) proteins. We used cultured myofibroblast-like cells obtained by outgrowth from explants of rat kidney cortex to study the effects and relevant signaling pathway of connective tissue growth factor (CTGF) on cell proliferation and ECM production. Exogenous CTGF stimulated proliferation of myofibroblast-like cells in a dose- and time-dependent manner. CTGF also increased the secretion of fibronectin and collagen I protein in the supernatant medium. Nevertheless, CTGF did not affect matrix-degrading metalloproteinases-2 and -9 activities in supernatant medium measured by gelatin zymography. CTGF induced activation of extracellular signal-regulated protein kinase (ERK)1/2 mitogen-activated protein kinase pathway as early as 5 minutes. Inhibition of ERK1/2 activation with PD98059 completely blocked CTGF-induced cell proliferation as well as secretion of fibronectin and collagen I protein. The above results indicate that CTGF triggers cell proliferation and production of ECM proteins in cultured myofibroblast-like cells through the ERK1/2 mitogen-activated protein kinase pathway.

语种英语
WOS记录号WOS:000255666500011
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60122
专题北京大学第一临床医学院_肾脏内科
北京大学基础医学院
北京大学第一临床医学院_放射治疗科
北京大学第一临床医学院_药剂科
作者单位1.Peking Univ, Inst Nephrol, Beijing 100034, Peoples R China
2.Peking Univ, Div Nephrol, Hosp 1, Beijing 100034, Peoples R China
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GB/T 7714
Gao, Xuxia,Li, Jingzi,Huang, Haichang,et al. Connective tissue growth factor stimulates renal cortical myofibroblast-like cell proliferation and matrix protein production[J]. WOUND REPAIR AND REGENERATION,2008,16(3):408-415.
APA Gao, Xuxia,Li, Jingzi,Huang, Haichang,&Li, Xiaomei.(2008).Connective tissue growth factor stimulates renal cortical myofibroblast-like cell proliferation and matrix protein production.WOUND REPAIR AND REGENERATION,16(3),408-415.
MLA Gao, Xuxia,et al."Connective tissue growth factor stimulates renal cortical myofibroblast-like cell proliferation and matrix protein production".WOUND REPAIR AND REGENERATION 16.3(2008):408-415.
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