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学科主题临床医学
Morphological characteristics of the lumbosacral spinal cord urogenital center in Sprague Dawley rats
Zhou, Mouwang1; Wang, Wenting1; Chen, Qingshan2
关键词urogenital center morphology microsurgical anatomy Sprague Dawley rats
刊名NEURAL REGENERATION RESEARCH
2009-12-01
DOI10.3969/j.issn.1673-5374.2009.12.023
4期:12页:1099-1103
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Neurosciences
研究领域[WOS]Cell Biology ; Neurosciences & Neurology
关键词[WOS]CENTRAL-NERVOUS-SYSTEM ; URINARY-BLADDER ; HORSERADISH-PEROXIDASE ; REFLEX PATHWAY ; INNERVATION ; INJURY ; MICTURITION ; AFFERENTS ; TRANSPORT ; NEURONS
英文摘要

BACKGROUND: Studies have demonstrated that selective innervation of the sacral nerve tract to the bladder plays an important role in bladder functional reconstruction following spinal cord injury. However, there are very few studies reporting detailed morphological characteristics of urogenital center and lumbosacral nerve roots.

OBJECTIVE: To analyze the spinal cord segment of the lumbosacral spinal cord urogenital center, and to observe morphological characteristics.

DESIGN, TIME AND SETTING: A neuroanatomical study was performed at the Laboratory of Neuroanatomy, Peking University Health Science Center between September 2007 and March 2008.

MATERIALS: Horseradish peroxidase-conjugated cholera toxin B subunit (CB-HRP) was purchased from Sigma, USA; surgical microscope was purchased from Zhenjian Zhongtian Optical Instrument, Jiangsu Province, China; BCL-420 biological and functional experimental system was purchased from Taimeng Science and Technology, Sichuan Province, China.

METHODS: A total of 36 adult, Sprague Dawley rats were randomly assigned to groups A (n = 10), B (n 10), C (n = 10), and D (n = 6). CB-HRP (3%, 10-15 mu L) was injected into the bladder detrusor muscle (group A), external urethral sphincter (group B), and perineal muscles (group C), respectively. Rats in group D were not given any treatments.

MAIN OUTCOME MEASURES: At 72 hours after CB-HRP injection, CB-HRP-positive neurons were analyzed in lumbosacral segments using 3, 3′, 5, 5′-tetramethylbenzidine staining and an Olympus optic microscope, while anatomical structures in the respective spinal nerve tract were observed using a surgical microscope.

RESULTS: CB-HRP-positive neurons were distributed in the L-6-S-1 segments of the spinal cord, and neurons primarily innervating the bladder detrusor muscle were located at the sacral parasympathetic nucleus and the intermediolateral nucleus. In addition, neurons that primarily innervate the external urethral sphincter and perineal muscles were observed in the ventrolateral portion (Onuf′s nucleus). The lumbar-sacral nerve roots were composed of varying nerve tracts, i.e., they were typically divided into 1-2 sub-bundles, and the sub-bundles were then divided into 2-3 tiny bundles. There were extensive fibro-connections between the rootlets.

CONCLUSION: The urogenital center in Sprague Dawley rats was located in the L-6-S-1 segments of the spinal cord, and the rootlets were clearly observed. Therefore, this rat experimental model could be utilized for highly selective anterior/posterior rhizotomy.

语种英语
WOS记录号WOS:000273468500023
项目编号30672096star
资助机构National Natural Science Foundation of China
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60152
专题北京大学第三临床医学院_康复医学科
北京大学基础医学院
作者单位1.Peking Univ, Hosp 3, Dept Rehabil Med, Beijing 100191, Peoples R China
2.Peking Univ, Dept Anat & Histol & Embryol, Hlth Sci Ctr, Beijing 100191, Peoples R China
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GB/T 7714
Zhou, Mouwang,Wang, Wenting,Chen, Qingshan. Morphological characteristics of the lumbosacral spinal cord urogenital center in Sprague Dawley rats[J]. NEURAL REGENERATION RESEARCH,2009,4(12):1099-1103.
APA Zhou, Mouwang,Wang, Wenting,&Chen, Qingshan.(2009).Morphological characteristics of the lumbosacral spinal cord urogenital center in Sprague Dawley rats.NEURAL REGENERATION RESEARCH,4(12),1099-1103.
MLA Zhou, Mouwang,et al."Morphological characteristics of the lumbosacral spinal cord urogenital center in Sprague Dawley rats".NEURAL REGENERATION RESEARCH 4.12(2009):1099-1103.
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