|Morphological characteristics of the lumbosacral spinal cord urogenital center in Sprague Dawley rats|
|Zhou, Mouwang1; Wang, Wenting1; Chen, Qingshan2|
|关键词||urogenital center morphology microsurgical anatomy Sprague Dawley rats|
|刊名||NEURAL REGENERATION RESEARCH|
|WOS标题词||Science & Technology|
|类目[WOS]||Cell Biology ; Neurosciences|
|研究领域[WOS]||Cell Biology ; Neurosciences & Neurology|
|关键词[WOS]||CENTRAL-NERVOUS-SYSTEM ; URINARY-BLADDER ; HORSERADISH-PEROXIDASE ; REFLEX PATHWAY ; INNERVATION ; INJURY ; MICTURITION ; AFFERENTS ; TRANSPORT ; NEURONS|
BACKGROUND: Studies have demonstrated that selective innervation of the sacral nerve tract to the bladder plays an important role in bladder functional reconstruction following spinal cord injury. However, there are very few studies reporting detailed morphological characteristics of urogenital center and lumbosacral nerve roots.
OBJECTIVE: To analyze the spinal cord segment of the lumbosacral spinal cord urogenital center, and to observe morphological characteristics.
DESIGN, TIME AND SETTING: A neuroanatomical study was performed at the Laboratory of Neuroanatomy, Peking University Health Science Center between September 2007 and March 2008.
MATERIALS: Horseradish peroxidase-conjugated cholera toxin B subunit (CB-HRP) was purchased from Sigma, USA; surgical microscope was purchased from Zhenjian Zhongtian Optical Instrument, Jiangsu Province, China; BCL-420 biological and functional experimental system was purchased from Taimeng Science and Technology, Sichuan Province, China.
METHODS: A total of 36 adult, Sprague Dawley rats were randomly assigned to groups A (n = 10), B (n 10), C (n = 10), and D (n = 6). CB-HRP (3%, 10-15 mu L) was injected into the bladder detrusor muscle (group A), external urethral sphincter (group B), and perineal muscles (group C), respectively. Rats in group D were not given any treatments.
MAIN OUTCOME MEASURES: At 72 hours after CB-HRP injection, CB-HRP-positive neurons were analyzed in lumbosacral segments using 3, 3′, 5, 5′-tetramethylbenzidine staining and an Olympus optic microscope, while anatomical structures in the respective spinal nerve tract were observed using a surgical microscope.
RESULTS: CB-HRP-positive neurons were distributed in the L-6-S-1 segments of the spinal cord, and neurons primarily innervating the bladder detrusor muscle were located at the sacral parasympathetic nucleus and the intermediolateral nucleus. In addition, neurons that primarily innervate the external urethral sphincter and perineal muscles were observed in the ventrolateral portion (Onuf′s nucleus). The lumbar-sacral nerve roots were composed of varying nerve tracts, i.e., they were typically divided into 1-2 sub-bundles, and the sub-bundles were then divided into 2-3 tiny bundles. There were extensive fibro-connections between the rootlets.
CONCLUSION: The urogenital center in Sprague Dawley rats was located in the L-6-S-1 segments of the spinal cord, and the rootlets were clearly observed. Therefore, this rat experimental model could be utilized for highly selective anterior/posterior rhizotomy.
|资助机构||National Natural Science Foundation of China|
|作者单位||1.Peking Univ, Hosp 3, Dept Rehabil Med, Beijing 100191, Peoples R China|
2.Peking Univ, Dept Anat & Histol & Embryol, Hlth Sci Ctr, Beijing 100191, Peoples R China
|Zhou, Mouwang,Wang, Wenting,Chen, Qingshan. Morphological characteristics of the lumbosacral spinal cord urogenital center in Sprague Dawley rats[J]. NEURAL REGENERATION RESEARCH,2009,4(12):1099-1103.|
|APA||Zhou, Mouwang,Wang, Wenting,&Chen, Qingshan.(2009).Morphological characteristics of the lumbosacral spinal cord urogenital center in Sprague Dawley rats.NEURAL REGENERATION RESEARCH,4(12),1099-1103.|
|MLA||Zhou, Mouwang,et al."Morphological characteristics of the lumbosacral spinal cord urogenital center in Sprague Dawley rats".NEURAL REGENERATION RESEARCH 4.12(2009):1099-1103.|