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学科主题: 基础医学
题名:
Two C-terminal peptides of human CKLF1 interact with the chemokine receptor CCR4
作者: Wang, Ying1; Zhang, Yingmei1; Han, Wenling1; Li, Dan2; Tian, Linjie2; Yin, Caihua2; Ma, Dalong1,2
关键词: chemokine ; CKLF1 ; TARC/CCL17 ; CCR4 ; ligand ; peptide
刊名: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
发表日期: 2008
DOI: 10.1016/j.biocel.2007.10.028
卷: 40, 期:5, 页:909-919
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: CHEMOKINE-LIKE FACTOR-1 ; MONOCYTE CHEMOATTRACTANT PROTEIN-1 ; MOLECULAR-CLONING ; RECEPTOR 4 ; GLYCOSAMINOGLYCAN-BINDING ; FUNCTIONAL LIGAND ; ALPHA-HELIX ; SITE ; IDENTIFICATION ; ANAPHYLATOXIN
英文摘要:

Human chemokine-like factor 1 (CKLF1) exhibits chemotactic effects on leukocytes. A previous study demonstrated that CKLF1 is a functional ligand for human CC chemokine receptor 4 (CCR4). In this study, N-terminal amino acid sequencing of secreted CKLF1 protein showed that it contains at least two peptides, C27 and C19. To examine whether C27 or C19 play a role via CCR4, C27 and C 19 were chemically synthesized and analyzed by chemotaxis, calcium mobilization, and receptor internalization assays in CCR4-tranfected HEK293 cells or Hut78 cells. The chemotaxis assay showed that C27 could induce chemotaxis to CCR4-transfected HEK293 cells or Hut78 cells while C19 had weaker chemotactic activity, especially in Hut78 cells. C27- or C19-induced chemotaxis was abolished by pertussis toxin, suggesting the involvement of a Gi/o pathway. C27- or C19-induced chemotaxis was also inhibited by an antagonist of CCR4 that show good binding potency, excellent chemotaxis inhibitory activity and selectivity toward CCR4, suggesting. that their chemotactic activity specifically involved CCR4. The chemotactic response of CCR4-tranfected HEK293 cells to C27 or C19 was markedly inhibited by preincubation with TARC/CCL17. TARC/CCL17 effectively desensitized the calcium mobilization induced by C27 or C19. Similarly, both of C27 or C19 also desensitized the calcium mobilization and chemotaxis of CCR4-tranfected HEK293 cells in response to TARC/CCL17, suggesting that they might interact with a common receptor. Both C27- and C19-induced clear internalization of CCR4-EGFP. These results confirm that the secreted peptides of CKLF1, C27 and C19, have functional activation via CCR4. (C) 2007 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000254980700010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60159
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Ctr Human Dis Genome, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Med Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Wang, Ying,Zhang, Yingmei,Han, Wenling,et al. Two C-terminal peptides of human CKLF1 interact with the chemokine receptor CCR4[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2008,40(5):909-919.
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