|PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells|
|Lei, Bin1,2; Wan, Bo1,2; Peng, Jian1; Yang, Yu1; Lv, Daojun2; Zhou, Xuming2; Shu, Fangpeng2; Li, Fei2; Zhong, Liren2; Wu, Huayan2; Mao, Xiangming1,2|
|关键词||testis infertility male Sertoli cell-only syndrome phosphoribosylpyrophosphate synthetase superactivity p53|
|刊名||JOURNAL OF UROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Urology & Nephrology|
|研究领域[WOS]||Urology & Nephrology|
|关键词[WOS]||SINGLE-NUCLEOTIDE POLYMORPHISMS ; TESTICULAR SPERM EXTRACTION ; MEDIATED BCL-2 ACTIVATION ; B16F-10 MELANOMA-CELLS ; GENETIC RISK-FACTOR ; ONLY SYNDROME ; PHOSPHORIBOSYLPYROPHOSPHATE SYNTHETASE ; NONOBSTRUCTIVE AZOOSPERMIA ; JAPANESE PATIENTS ; INFERTILE MEN|
Purpose: Sertoli-cell only syndrome is one of the reasons for male infertility but its pathogenesis remains unclear. PRPS2, a subset of PRS, is reported to be a potential protein associated with Sertoli-cell only syndrome. In this study we further investigated the correlation between PRPS2 and Sertoli-cell only syndrome, and evaluated the effect of PRPS2 expression on apoptosis of TM4 Sertoli cells.
Materials and Methods: PRPS2 expression was detected in patients with Sertoli-cell only syndrome and normal spermatogenesis, and in Sertoli-cell only syndrome mouse models by immunohistochemistry, quantitative reverse transcription-polymerase chain reaction and Western blot. PRPS2 expression in TM4 Sertoli cells was then down-regulated and up-regulated by lentivirus vectors. The effect of PRPS2 expression on cell apoptosis and cell cycle transition was evaluated by flow cytometry.
Results: PRPS2 expression in patients with Sertoli-cell only syndrome was significantly greater than in those with normal spermatogenesis. A significant increase in PRPS2 expression was observed in Sertoli-cell only syndrome mouse models. PRPS2 over expression significantly inhibited cell apoptosis and promoted cell cycle transition in TM4 Sertoli cells. However, PRPS2 downregulation showed a reverse effect. Moreover, results revealed that PRPS2 over expression inhibited cell apoptosis via the p53/Bcl-2/caspase-9/caspase-3/ caspase-6/caspase-7 signaling pathway.
Conclusions: PRPS2 expression correlates with Sertoli-cell only syndrome and inhibits the apoptosis of TM4 Sertoli cells via the p53/Bcl-2/caspases signaling pathway.
|项目编号||81270743 ; 2013B021800147 ; 20140323125307|
|资助机构||National Natural Science Foundation of China ; Science and Technology Projects of Guangdong Province ; Knowledge Innovation Project of Shenzhen|
|作者单位||1.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen, Guangdong, Peoples R China|
2.Southern Med Univ, Nanfang Hosp, Guangzhou, Guangdong, Peoples R China
|Lei, Bin,Wan, Bo,Peng, Jian,et al. PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells[J]. JOURNAL OF UROLOGY,2015,194(5):1491-1497.|
|APA||Lei, Bin.,Wan, Bo.,Peng, Jian.,Yang, Yu.,Lv, Daojun.,...&Mao, Xiangming.(2015).PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells.JOURNAL OF UROLOGY,194(5),1491-1497.|
|MLA||Lei, Bin,et al."PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells".JOURNAL OF UROLOGY 194.5(2015):1491-1497.|