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学科主题: 临床医学
题名:
PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells
作者: Lei, Bin1,2; Wan, Bo1,2; Peng, Jian1; Yang, Yu1; Lv, Daojun2; Zhou, Xuming2; Shu, Fangpeng2; Li, Fei2; Zhong, Liren2; Wu, Huayan2; Mao, Xiangming1,2
关键词: testis ; infertility ; male ; Sertoli cell-only syndrome ; phosphoribosylpyrophosphate synthetase superactivity ; p53
刊名: JOURNAL OF UROLOGY
发表日期: 2015-11-01
DOI: 10.1016/j.juro.2015.04.116
卷: 194, 期:5, 页:1491-1497
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: SINGLE-NUCLEOTIDE POLYMORPHISMS ; TESTICULAR SPERM EXTRACTION ; MEDIATED BCL-2 ACTIVATION ; B16F-10 MELANOMA-CELLS ; GENETIC RISK-FACTOR ; ONLY SYNDROME ; PHOSPHORIBOSYLPYROPHOSPHATE SYNTHETASE ; NONOBSTRUCTIVE AZOOSPERMIA ; JAPANESE PATIENTS ; INFERTILE MEN
英文摘要:

Purpose: Sertoli-cell only syndrome is one of the reasons for male infertility but its pathogenesis remains unclear. PRPS2, a subset of PRS, is reported to be a potential protein associated with Sertoli-cell only syndrome. In this study we further investigated the correlation between PRPS2 and Sertoli-cell only syndrome, and evaluated the effect of PRPS2 expression on apoptosis of TM4 Sertoli cells.

Materials and Methods: PRPS2 expression was detected in patients with Sertoli-cell only syndrome and normal spermatogenesis, and in Sertoli-cell only syndrome mouse models by immunohistochemistry, quantitative reverse transcription-polymerase chain reaction and Western blot. PRPS2 expression in TM4 Sertoli cells was then down-regulated and up-regulated by lentivirus vectors. The effect of PRPS2 expression on cell apoptosis and cell cycle transition was evaluated by flow cytometry.

Results: PRPS2 expression in patients with Sertoli-cell only syndrome was significantly greater than in those with normal spermatogenesis. A significant increase in PRPS2 expression was observed in Sertoli-cell only syndrome mouse models. PRPS2 over expression significantly inhibited cell apoptosis and promoted cell cycle transition in TM4 Sertoli cells. However, PRPS2 downregulation showed a reverse effect. Moreover, results revealed that PRPS2 over expression inhibited cell apoptosis via the p53/Bcl-2/caspase-9/caspase-3/ caspase-6/caspase-7 signaling pathway.

Conclusions: PRPS2 expression correlates with Sertoli-cell only syndrome and inhibits the apoptosis of TM4 Sertoli cells via the p53/Bcl-2/caspases signaling pathway.

语种: 英语
所属项目编号: 81270743 ; 2013B021800147 ; 20140323125307
项目资助者: National Natural Science Foundation of China ; Science and Technology Projects of Guangdong Province ; Knowledge Innovation Project of Shenzhen
WOS记录号: WOS:000366011100022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60163
Appears in Collections:北京大学深圳医院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen, Guangdong, Peoples R China
2.Southern Med Univ, Nanfang Hosp, Guangzhou, Guangdong, Peoples R China

Recommended Citation:
Lei, Bin,Wan, Bo,Peng, Jian,et al. PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells[J]. JOURNAL OF UROLOGY,2015,194(5):1491-1497.
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