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学科主题: 临床医学
题名:
Enantiomeric separation of beta-blockers by HPLC using (R)-1-naphthylglycine and 3,5-dinitrobenzoic acid as chiral stationary phase
作者: Zhang, XH; Ouyang, J; Baeyens, WRG; Zhai, SD; Yang, YP; Huang, GM
关键词: enantiomeric separation ; Pirkle-type stationary phase ; metoprolol ; bisoprolol
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2003-04-10
DOI: 10.1016/S0731-7085(02)00697-0
卷: 31, 期:6, 页:1047-1057
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; SUPERCRITICAL-FLUID CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; METOPROLOL ENANTIOMERS ; BLOCKING-AGENTS ; HUMAN URINE ; PROPRANOLOL ; PLASMA ; ENANTIOSEPARATIONS ; DERIVATIVES
英文摘要:

Direct liquid chromatographic separations of the enantiomers of metoprolol and bisoprolol have been developed, using (R)-1-naphthylglycine and 3,5-dinitrobenzoic acid as chiral stationary phase (CSP). The separations were achieved in a normal phase system employing a mobile phase containing n-hexane, 1,2-dichloroethane and methanol. Column efficiency was strongly dependent on the composition of the mobile phase. The eluent contents of methanol and of 1,2-dichloroethane were optimized, and so was flow-rate and column temperature. Under the optimal conditions, linear responses for (R)-metoprolol and (S)-metoprolol are obtained in the range of 0.079-1.38 and 0.015-5.80 mg/ml, with detection limits of 0.008 and 0.002 mg/ml, respectively. As for bisoprolol, the linear ranges of (R)isomer and (S)-isomer are 0.05-1.31 and 0.02-1.00 mg/ml with detection limits of 0.001 and 0.008 mg/ml, respectively. The relative standard deviation (R.S.D.) of each enantiomer did not exceed 0.90%. The method has been successfully applied to the determination of enantiomers in pharmaceuticals. (C) 2003 Elsevier Science B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000182275400002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60170
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Beijing 100831, Peoples R China
2.Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China
3.State Univ Ghent, Lab Drug Qual Control, Dept Pharmaceut Anal, Fac Pharmaceut Sci, B-9000 Ghent, Belgium

Recommended Citation:
Zhang, XH,Ouyang, J,Baeyens, WRG,et al. Enantiomeric separation of beta-blockers by HPLC using (R)-1-naphthylglycine and 3,5-dinitrobenzoic acid as chiral stationary phase[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2003,31(6):1047-1057.
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