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Enantiomeric separation of beta-blockers by HPLC using (R)-1-naphthylglycine and 3,5-dinitrobenzoic acid as chiral stationary phase
Zhang, XH; Ouyang, J; Baeyens, WRG; Zhai, SD; Yang, YP; Huang, GM
关键词enantiomeric separation Pirkle-type stationary phase metoprolol bisoprolol
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2003-04-10
DOI10.1016/S0731-7085(02)00697-0
31期:6页:1047-1057
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; SUPERCRITICAL-FLUID CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; METOPROLOL ENANTIOMERS ; BLOCKING-AGENTS ; HUMAN URINE ; PROPRANOLOL ; PLASMA ; ENANTIOSEPARATIONS ; DERIVATIVES
英文摘要

Direct liquid chromatographic separations of the enantiomers of metoprolol and bisoprolol have been developed, using (R)-1-naphthylglycine and 3,5-dinitrobenzoic acid as chiral stationary phase (CSP). The separations were achieved in a normal phase system employing a mobile phase containing n-hexane, 1,2-dichloroethane and methanol. Column efficiency was strongly dependent on the composition of the mobile phase. The eluent contents of methanol and of 1,2-dichloroethane were optimized, and so was flow-rate and column temperature. Under the optimal conditions, linear responses for (R)-metoprolol and (S)-metoprolol are obtained in the range of 0.079-1.38 and 0.015-5.80 mg/ml, with detection limits of 0.008 and 0.002 mg/ml, respectively. As for bisoprolol, the linear ranges of (R)isomer and (S)-isomer are 0.05-1.31 and 0.02-1.00 mg/ml with detection limits of 0.001 and 0.008 mg/ml, respectively. The relative standard deviation (R.S.D.) of each enantiomer did not exceed 0.90%. The method has been successfully applied to the determination of enantiomers in pharmaceuticals. (C) 2003 Elsevier Science B.V. All rights reserved.

语种英语
WOS记录号WOS:000182275400002
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60170
专题北京大学第三临床医学院
作者单位1.Peking Univ, Hosp 3, Beijing 100831, Peoples R China
2.Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China
3.State Univ Ghent, Lab Drug Qual Control, Dept Pharmaceut Anal, Fac Pharmaceut Sci, B-9000 Ghent, Belgium
推荐引用方式
GB/T 7714
Zhang, XH,Ouyang, J,Baeyens, WRG,et al. Enantiomeric separation of beta-blockers by HPLC using (R)-1-naphthylglycine and 3,5-dinitrobenzoic acid as chiral stationary phase[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2003,31(6):1047-1057.
APA Zhang, XH,Ouyang, J,Baeyens, WRG,Zhai, SD,Yang, YP,&Huang, GM.(2003).Enantiomeric separation of beta-blockers by HPLC using (R)-1-naphthylglycine and 3,5-dinitrobenzoic acid as chiral stationary phase.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,31(6),1047-1057.
MLA Zhang, XH,et al."Enantiomeric separation of beta-blockers by HPLC using (R)-1-naphthylglycine and 3,5-dinitrobenzoic acid as chiral stationary phase".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 31.6(2003):1047-1057.
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