|Examination of Smad2 and Smad4 copy-number variations in skin cancers|
|Shao, Yong1,2,3; Zhang, Jie1,2,3; Zhang, Richu4; Wan, Jun1,2,5; Zhang, Wei1,5,6; Yu, Bo1,2,3|
|关键词||Smad2 Smad4 Copy number variations Basal cell carcinoma Squamous cell carcinoma Actinic keratosis|
|刊名||CLINICAL & TRANSLATIONAL ONCOLOGY|
|WOS标题词||Science & Technology|
|关键词[WOS]||TIME QUANTITATIVE PCR ; TGF-BETA ; GENOME ; TUMORS ; GENE|
Background Smad2 and Smad4 transcription factors were identified as the signalling mediators of transforming growth factor beta (TGF beta) pathway. Copy number variations (CNVs) have been discovered to have phenotypic consequences and be associated with various types of cancers. CNVs of Smad2 and Smad4 were found to be associated with cancer pathogenesis in the recent array-based study. However, no such study has been performed in skin cancer yet. In this study, we aim to examine the CNVs of Smad2 and Smad4 in skin samples.
Methods A total of 195 paired samples including basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and actinic keratosis (AK) were included. Real-time PCR was used for the quantification of Smad2 and Smad4 copy numbers.
Results CNVs of Smad2 showed statistical differences between cancer samples (both SCC and BCC) and normal tissues (p<0.05). For Smad4, statistical difference was observed only in SCC samples (p=0.014), but not in BCC and AK samples (p=0.173 and 0.314, respectively). Association analysis showed that the frequencies of Smad2 and Smad4 CNVs were correlated with the severity of skin abnormalities (p=0.002 for Smad2 and p=0.029 for Smad4).
Conclusions CNVs of Smad2 are associated with SCC and BCC, while CNVs of Smad4 are associated with SCC but not BCC.
|资助机构||Shenzhen Science and Technology Project|
|作者单位||1.Taizhou Municipal Hosp, Dept Gen Surg, Taizhou, Zhejiang, Peoples R China|
2.Shenzhen PKU HKUST Med Ctr, Shenzhen Key Lab Translat Med Dermatol, Shenzhen 518036, Guangdong, Peoples R China
3.Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen 518036, Guangdong, Peoples R China
4.Peking Univ, Dept Dermatol, Shenzhen Hosp, Shenzhen, Guangdong, Peoples R China
5.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China
6.Ji Nan Univ, JNU HKUST Joint Lab, Jinan, Guangdong, Peoples R China
|Shao, Yong,Zhang, Jie,Zhang, Richu,et al. Examination of Smad2 and Smad4 copy-number variations in skin cancers[J]. CLINICAL & TRANSLATIONAL ONCOLOGY,2012,14(2):138-142.|
|APA||Shao, Yong,Zhang, Jie,Zhang, Richu,Wan, Jun,Zhang, Wei,&Yu, Bo.(2012).Examination of Smad2 and Smad4 copy-number variations in skin cancers.CLINICAL & TRANSLATIONAL ONCOLOGY,14(2),138-142.|
|MLA||Shao, Yong,et al."Examination of Smad2 and Smad4 copy-number variations in skin cancers".CLINICAL & TRANSLATIONAL ONCOLOGY 14.2(2012):138-142.|