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Protective Effect of 1,25-Dihydroxyvitamin D3 on Lipopolysaccharide-Induced Intestinal Epithelial Tight Junction Injury in Caco-2 Cell Monolayers
Chen, Shan-Wen; Wang, Peng-Yuan; Zhu, Jing; Chen, Guo-Wei; Zhang, Jun-Ling; Chen, Zi-Yi; Zuo, Shuai; Liu, Yu-Cun; Pan, Yi-Sheng
关键词lipopolysaccharide 1,25(OH)2D3 VDR tight junction transepithelial electrical resistance ZO-1 Claudin-1 Occludin
刊名INFLAMMATION
2015-02-01
DOI10.1007/s10753-014-0041-9
38期:1页:375-383
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Immunology
研究领域[WOS]Cell Biology ; Immunology
关键词[WOS]INFLAMMATORY-BOWEL-DISEASE ; VITAMIN-D-RECEPTOR ; NECROTIZING ENTEROCOLITIS ; BARRIER FUNCTION ; MUCOSAL BARRIER ; ORGAN FAILURE ; PERMEABILITY ; ENDOTOXEMIA ; COLITIS ; PROTEIN
英文摘要

Lipopolysaccharide was found to be elevated in the plasma of necrotizing enterocolitis (NEC) and inflammatory bowel disease (IBD) patients and may play an important role in the pathogenesis and propagation of these intestinal diseases. To illustrate the destructive effect of lipopolysaccharide (LPS) and to test the protective effect of 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) on LPS-induced barrier injury, an in vitro intestinal epithelia barrier model was established with Caco-2 monolayers and treated with clinically relevant concentrations (1-10 ng/ml) of LPS with or without 1,25(OH)2D3. Transepithelial electrical resistance (TEER) and FITC-Dextran 40kda (FD-40) flux were measured to reflect monolayer permeability. We found that LPS at clinically relevant concentrations increased intestinal permeability by downregulating and redistributing tight junction (TJ) proteins. 1,25(OH)2D3 added at baseline or at day 4 abrogated the destructive effect of LPS on monolayer permeability by restoring the expression and localization of TJ proteins. LPS, at clinically relevant concentrations, also downregulated the expression of vitamin D receptor (VDR); 1,25 (OH)2D3, however, could restore the expression of VDR. Our findings illustrate the mechanism underlying the destructive effect of clinically relevant concentrations of LPS on intestinal TJ barrier and provide evidence for the clinical application of vitamin D in LPS-related intestinal barrier dysfunction.

语种英语
WOS记录号WOS:000349015900041
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60218
专题北京大学第一临床医学院
作者单位Peking Univ, Peking Univ Hosp 1, Div Gen Surg, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Chen, Shan-Wen,Wang, Peng-Yuan,Zhu, Jing,et al. Protective Effect of 1,25-Dihydroxyvitamin D3 on Lipopolysaccharide-Induced Intestinal Epithelial Tight Junction Injury in Caco-2 Cell Monolayers[J]. INFLAMMATION,2015,38(1):375-383.
APA Chen, Shan-Wen.,Wang, Peng-Yuan.,Zhu, Jing.,Chen, Guo-Wei.,Zhang, Jun-Ling.,...&Pan, Yi-Sheng.(2015).Protective Effect of 1,25-Dihydroxyvitamin D3 on Lipopolysaccharide-Induced Intestinal Epithelial Tight Junction Injury in Caco-2 Cell Monolayers.INFLAMMATION,38(1),375-383.
MLA Chen, Shan-Wen,et al."Protective Effect of 1,25-Dihydroxyvitamin D3 on Lipopolysaccharide-Induced Intestinal Epithelial Tight Junction Injury in Caco-2 Cell Monolayers".INFLAMMATION 38.1(2015):375-383.
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