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学科主题: 临床医学
题名:
Protective Effect of 1,25-Dihydroxyvitamin D3 on Lipopolysaccharide-Induced Intestinal Epithelial Tight Junction Injury in Caco-2 Cell Monolayers
作者: Chen, Shan-Wen; Wang, Peng-Yuan; Zhu, Jing; Chen, Guo-Wei; Zhang, Jun-Ling; Chen, Zi-Yi; Zuo, Shuai; Liu, Yu-Cun; Pan, Yi-Sheng
关键词: lipopolysaccharide ; 1,25(OH)2D3 ; VDR ; tight junction ; transepithelial electrical resistance ; ZO-1 ; Claudin-1 ; Occludin
刊名: INFLAMMATION
发表日期: 2015-02-01
DOI: 10.1007/s10753-014-0041-9
卷: 38, 期:1, 页:375-383
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Immunology
研究领域[WOS]: Cell Biology ; Immunology
关键词[WOS]: INFLAMMATORY-BOWEL-DISEASE ; VITAMIN-D-RECEPTOR ; NECROTIZING ENTEROCOLITIS ; BARRIER FUNCTION ; MUCOSAL BARRIER ; ORGAN FAILURE ; PERMEABILITY ; ENDOTOXEMIA ; COLITIS ; PROTEIN
英文摘要:

Lipopolysaccharide was found to be elevated in the plasma of necrotizing enterocolitis (NEC) and inflammatory bowel disease (IBD) patients and may play an important role in the pathogenesis and propagation of these intestinal diseases. To illustrate the destructive effect of lipopolysaccharide (LPS) and to test the protective effect of 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) on LPS-induced barrier injury, an in vitro intestinal epithelia barrier model was established with Caco-2 monolayers and treated with clinically relevant concentrations (1-10 ng/ml) of LPS with or without 1,25(OH)2D3. Transepithelial electrical resistance (TEER) and FITC-Dextran 40kda (FD-40) flux were measured to reflect monolayer permeability. We found that LPS at clinically relevant concentrations increased intestinal permeability by downregulating and redistributing tight junction (TJ) proteins. 1,25(OH)2D3 added at baseline or at day 4 abrogated the destructive effect of LPS on monolayer permeability by restoring the expression and localization of TJ proteins. LPS, at clinically relevant concentrations, also downregulated the expression of vitamin D receptor (VDR); 1,25 (OH)2D3, however, could restore the expression of VDR. Our findings illustrate the mechanism underlying the destructive effect of clinically relevant concentrations of LPS on intestinal TJ barrier and provide evidence for the clinical application of vitamin D in LPS-related intestinal barrier dysfunction.

语种: 英语
WOS记录号: WOS:000349015900041
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60218
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: Peking Univ, Peking Univ Hosp 1, Div Gen Surg, Beijing 100034, Peoples R China

Recommended Citation:
Chen, Shan-Wen,Wang, Peng-Yuan,Zhu, Jing,et al. Protective Effect of 1,25-Dihydroxyvitamin D3 on Lipopolysaccharide-Induced Intestinal Epithelial Tight Junction Injury in Caco-2 Cell Monolayers[J]. INFLAMMATION,2015,38(1):375-383.
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