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学科主题: 临床医学
题名:
Reduced Expression of von Hippel-Lindau Protein Correlates with Decreased Apoptosis and High Chondrosarcoma Grade
作者: Chen, Changbao; Zhou, Hua; Liu, Xiaoguang; Liu, Zhongjun; Ma, Qingjun
刊名: JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
发表日期: 2011-10-05
DOI: 10.2106/JBJS.I.01553
卷: 93A, 期:19, 页:1833-1840
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Orthopedics ; Surgery
研究领域[WOS]: Orthopedics ; Surgery
关键词[WOS]: TUMOR-SUPPRESSOR PROTEIN ; RENAL-CELL CARCINOMA ; HYPOXIA-INDUCIBLE FACTOR-1-ALPHA ; CARTILAGE TUMORS ; BONE ; GENE ; PATHWAYS ; CANCER ; STABILIZATION ; NEUROBLASTOMA
英文摘要:

Background: Mutations and loss of the von Hippel-Lindau (VHL) tumor suppressor gene are associated with most renal cancers as well as several other types of human tumors, but the potential role of the VHL protein (pVHL) in patients with chondrosarcoma has not been characterized. The purpose of the present study was to investigate the expression profiles of pVHL in chondrosarcoma and its association with clinicopathologic parameters, Bax expression, the apoptosis index, and overall survival of patients with chondrosarcoma.

Methods: The messenger RNA (mRNA) and protein levels of VHL in fresh specimens from eight chondrosarcomas were studied with use of real-time polymerase chain reaction and Western blot, respectively. The protein expression of VHL and Bax was investigated by means of immunohistochemical analysis of,paraffin-embedded clinical specimens from seventeen benign cartilage tumors and thirty-four chondrosarcomas. The apoptosis index in chondrosarcoma was examined by means of the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) assay. Curves for overall survival were drawn according to the Kaplan-Meier method, and differences were analyzed with the log-rank test. The association of pVHL expression with the clinicopathologic parameters, Bax expression, apoptosis index, and overall survival for patients with chondrosarcoma was also analyzed.

Results: Levels of VHL protein (p = 0.005) and mRNA (p = 0.008) were significantly reduced in chondrosarcoma tissues as compared with the paired adjacent normal tissues. Immunohistochemical analysis showed decreased pVHL in a significantly higher proportion of chondrosarcomas (64.7%) than benign cartilage tumors (29.4%). pVHL expression was positively correlated with Bax expression and the a poptosis index in chondrosarcoma. Longitudinal studies of a cohort of patients with chondrosarcomas showed that decreased pVHL expression significantly correlated with increased tumor. grade (p = 0.026) but was not independently predictive of overall survival.

Conclusions: Reduced pVHL expression was associated with decreased apoptosis and increasing chondrosarcoma grade, but the relationship between these findings and chondrosarcoma pathogenesis requires further study.

语种: 英语
所属项目编号: 30571870
项目资助者: National Natural Sciences Foundation of China
WOS记录号: WOS:000295813100011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60225
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: Peking Univ, Dept Orthopaed Surg, Hosp 3, Beijing 100191, Peoples R China

Recommended Citation:
Chen, Changbao,Zhou, Hua,Liu, Xiaoguang,et al. Reduced Expression of von Hippel-Lindau Protein Correlates with Decreased Apoptosis and High Chondrosarcoma Grade[J]. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME,2011,93A(19):1833-1840.
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