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学科主题: 公共卫生
题名:
Regulation of dHAND protein expression by all-trans retinoic acid through ET-1/ETAR signaling in H9c2 cells
作者: Li, Weixin; Li, Yong
关键词: all-trans retinoic acid ; H9c2 cells ; dHAND ; ET-1 ; signal
刊名: JOURNAL OF CELLULAR BIOCHEMISTRY
发表日期: 2006-10-01
DOI: 10.1002/jcb.20940
卷: 99, 期:2, 页:478-484
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: CASCADE INVOLVING ENDOTHELIN-1 ; NEURAL CREST DEVELOPMENT ; CARDIAC DEVELOPMENT ; ARCH MESENCHYME ; GENE-EXPRESSION ; DISEASE ; MORPHOGENESIS
英文摘要:

dHAND is thought to be a cardiac-restricted transcription factor during embryonic development. Vertebrate heart development involves many transcription factors such as Nkx2.5, GATA, and tbx5. All-trans retinoic acid (AtRA), the oxidative metabolite of vitamin A, can regulate the expression of these factors to affect embryonic heart development. However, the action of atRA on the expression of dHAND is rarely reported. To clarify whether atRA regulate the dHAND expression, we exposed cultured H9c2 cells (rat embryonic cardiomyocytes) to atRA and detected the protein expression of dHAND by Western blot analysis. We observed atRA can regulate the dHAND expression in a dose- and time-dependent manner. AtRA also inhibited endothelin-1 (ET-1) expression in a time-dependent manner. Further studies revealed that pretreatment with 10 mu M BQ-123, a selective endothelin-1 receptor (ETAR) antagonist, for 2 h can significantly counteract the inhibition of 5 mu M atRA treatment for 2 h of dHAN D mRNA and protein expression. Taken together, these results suggest that atRA regulates dHAND expression by ET-1/ETAR signal transduction pathway in H9c2 cells. The mechanism of ET-1/ETAR signaling in controlling the level of dHAND protein is to reduce the levels of dHAND mRNA. It is possible for atRA to exert its cardiac teratogenesis during vertebrate embryonic development in this way.

语种: 英语
WOS记录号: WOS:000240758600014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60232
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Lab Dev Mol Biol, Beijing 100083, Peoples R China

Recommended Citation:
Li, Weixin,Li, Yong. Regulation of dHAND protein expression by all-trans retinoic acid through ET-1/ETAR signaling in H9c2 cells[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2006,99(2):478-484.
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