IR@PKUHSC  > 北京大学公共卫生学院
学科主题公共卫生
Regulation of dHAND protein expression by all-trans retinoic acid through ET-1/ETAR signaling in H9c2 cells
Li, Weixin; Li, Yong
关键词all-trans retinoic acid H9c2 cells dHAND ET-1 signal
刊名JOURNAL OF CELLULAR BIOCHEMISTRY
2006-10-01
DOI10.1002/jcb.20940
99期:2页:478-484
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]CASCADE INVOLVING ENDOTHELIN-1 ; NEURAL CREST DEVELOPMENT ; CARDIAC DEVELOPMENT ; ARCH MESENCHYME ; GENE-EXPRESSION ; DISEASE ; MORPHOGENESIS
英文摘要

dHAND is thought to be a cardiac-restricted transcription factor during embryonic development. Vertebrate heart development involves many transcription factors such as Nkx2.5, GATA, and tbx5. All-trans retinoic acid (AtRA), the oxidative metabolite of vitamin A, can regulate the expression of these factors to affect embryonic heart development. However, the action of atRA on the expression of dHAND is rarely reported. To clarify whether atRA regulate the dHAND expression, we exposed cultured H9c2 cells (rat embryonic cardiomyocytes) to atRA and detected the protein expression of dHAND by Western blot analysis. We observed atRA can regulate the dHAND expression in a dose- and time-dependent manner. AtRA also inhibited endothelin-1 (ET-1) expression in a time-dependent manner. Further studies revealed that pretreatment with 10 mu M BQ-123, a selective endothelin-1 receptor (ETAR) antagonist, for 2 h can significantly counteract the inhibition of 5 mu M atRA treatment for 2 h of dHAN D mRNA and protein expression. Taken together, these results suggest that atRA regulates dHAND expression by ET-1/ETAR signal transduction pathway in H9c2 cells. The mechanism of ET-1/ETAR signaling in controlling the level of dHAND protein is to reduce the levels of dHAND mRNA. It is possible for atRA to exert its cardiac teratogenesis during vertebrate embryonic development in this way.

语种英语
WOS记录号WOS:000240758600014
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60232
专题北京大学公共卫生学院
作者单位Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Lab Dev Mol Biol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Li, Weixin,Li, Yong. Regulation of dHAND protein expression by all-trans retinoic acid through ET-1/ETAR signaling in H9c2 cells[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2006,99(2):478-484.
APA Li, Weixin,&Li, Yong.(2006).Regulation of dHAND protein expression by all-trans retinoic acid through ET-1/ETAR signaling in H9c2 cells.JOURNAL OF CELLULAR BIOCHEMISTRY,99(2),478-484.
MLA Li, Weixin,et al."Regulation of dHAND protein expression by all-trans retinoic acid through ET-1/ETAR signaling in H9c2 cells".JOURNAL OF CELLULAR BIOCHEMISTRY 99.2(2006):478-484.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Weixin]的文章
[Li, Yong]的文章
百度学术
百度学术中相似的文章
[Li, Weixin]的文章
[Li, Yong]的文章
必应学术
必应学术中相似的文章
[Li, Weixin]的文章
[Li, Yong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。