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学科主题临床医学
Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells
Zhang, Hong-Mei; Zhang, Li-Wang; Ren, Jun; Fan, Li; Si, Xiao-Ming; Liu, Wen-Chao
关键词alpha-fetoprotein dendritic cells hepatocellular carcinoma immunotherapy
刊名CELLULAR IMMUNOLOGY
2006-02-01
DOI10.1016/j.cellimm.2006.05.004
239期:2页:144-150
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Immunology
研究领域[WOS]Cell Biology ; Immunology
关键词[WOS]HEPATOCELLULAR-CARCINOMA ; TUMOR RNA ; ANTIGEN ; IMMUNOTHERAPY ; VACCINATION ; CANCER ; MICE ; METASTASES ; REGRESSION ; MELANOMA
英文摘要

alpha-Fetoprotein (AFP) may be a possible target for a hepatocellular carcinoma (HCC)-specific vaccination. But some studies have demonstrated that dendritic cells (DCs) treated with AFP become dysfunctional. So in this study, we try to transfect AFP mRNA into DCs and observe the ability of DCs to induce AFP-specific CD4(+) and CD8(+) T cells. We hope that AFP can be processed and presented by DCs directly, rather than released to the cultures. So there will be no AFP negative effect on the function of DCs. In the study, immature DCs generated from peripheral blood mononuclear cells (PBMCs) of HLA-A2(+) HCC patients were transfected with AFP mRNA. Then the transfected, matured DCs were used to stimulate autologous T cells. The results showed that the expressions of membrane molecules of DCs after transfection were increased dramatically, and interleukin-12 (IL-12) p70 release in the supernatant was elevated significantly. There was only a minority of AFP release in the supernatants of transfected DCs. CTLs induced by the transfected DCs recognized HLA-matched AFP positive HepG2 cell line specifically and the AFP-specific proliferative T-cell responses could also be induced. These findings indicate that this AFP mRNA transfection strategy could generate fully functional DCs, which could induce specific T cells to recognize AFP(+) HCC cells. (c) 2006 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000239324100008
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60234
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Beijing Canc Hosp, Internal Dept, Sch Oncol, Beijing 100036, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Ctr Clin Oncol, Xian 710032, Peoples R China
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Zhang, Hong-Mei,Zhang, Li-Wang,Ren, Jun,et al. Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells[J]. CELLULAR IMMUNOLOGY,2006,239(2):144-150.
APA Zhang, Hong-Mei,Zhang, Li-Wang,Ren, Jun,Fan, Li,Si, Xiao-Ming,&Liu, Wen-Chao.(2006).Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells.CELLULAR IMMUNOLOGY,239(2),144-150.
MLA Zhang, Hong-Mei,et al."Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells".CELLULAR IMMUNOLOGY 239.2(2006):144-150.
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