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学科主题: 临床医学
题名:
Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells
作者: Zhang, Hong-Mei; Zhang, Li-Wang; Ren, Jun; Fan, Li; Si, Xiao-Ming; Liu, Wen-Chao
关键词: alpha-fetoprotein ; dendritic cells ; hepatocellular carcinoma ; immunotherapy
刊名: CELLULAR IMMUNOLOGY
发表日期: 2006-02-01
DOI: 10.1016/j.cellimm.2006.05.004
卷: 239, 期:2, 页:144-150
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Immunology
研究领域[WOS]: Cell Biology ; Immunology
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ; TUMOR RNA ; ANTIGEN ; IMMUNOTHERAPY ; VACCINATION ; CANCER ; MICE ; METASTASES ; REGRESSION ; MELANOMA
英文摘要:

alpha-Fetoprotein (AFP) may be a possible target for a hepatocellular carcinoma (HCC)-specific vaccination. But some studies have demonstrated that dendritic cells (DCs) treated with AFP become dysfunctional. So in this study, we try to transfect AFP mRNA into DCs and observe the ability of DCs to induce AFP-specific CD4(+) and CD8(+) T cells. We hope that AFP can be processed and presented by DCs directly, rather than released to the cultures. So there will be no AFP negative effect on the function of DCs. In the study, immature DCs generated from peripheral blood mononuclear cells (PBMCs) of HLA-A2(+) HCC patients were transfected with AFP mRNA. Then the transfected, matured DCs were used to stimulate autologous T cells. The results showed that the expressions of membrane molecules of DCs after transfection were increased dramatically, and interleukin-12 (IL-12) p70 release in the supernatant was elevated significantly. There was only a minority of AFP release in the supernatants of transfected DCs. CTLs induced by the transfected DCs recognized HLA-matched AFP positive HepG2 cell line specifically and the AFP-specific proliferative T-cell responses could also be induced. These findings indicate that this AFP mRNA transfection strategy could generate fully functional DCs, which could induce specific T cells to recognize AFP(+) HCC cells. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000239324100008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60234
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Beijing Canc Hosp, Internal Dept, Sch Oncol, Beijing 100036, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Ctr Clin Oncol, Xian 710032, Peoples R China

Recommended Citation:
Zhang, Hong-Mei,Zhang, Li-Wang,Ren, Jun,et al. Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells[J]. CELLULAR IMMUNOLOGY,2006,239(2):144-150.
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