IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Identification of Pseudallescheria and Scedosporium Species by Three Molecular Methods
Lu, Qiaoyun1,2,3; van den Ende, A. H. G. Gerrits3; Bakkers, J. M. J. E.4; Sun, Jiufeng3,5; Lackner, M.3,6,7; Najafzadeh, M. J.3,8,9; Melchers, W. J. G.4; Li, Ruoyu1,2; de Hoog, G. S.1,2,3,8
刊名JOURNAL OF CLINICAL MICROBIOLOGY
2011-03-01
DOI10.1128/JCM.01813-10
49期:3页:960-967
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
资助者Health Profession of China ; 11th Five-Year Key Special Subject for Science and Technical Research of China ; China Scholarship Council ; Health Profession of China ; 11th Five-Year Key Special Subject for Science and Technical Research of China ; China Scholarship Council
研究领域[WOS]Microbiology
关键词[WOS]MEDIATED ISOTHERMAL AMPLIFICATION ; REVERSE LINE BLOT ; CYSTIC-FIBROSIS ; APIOSPERMUM INFECTION ; SELECTIVE ISOLATION ; BOYDII COMPLEX ; PCR ; VALIDATION ; SAMPLES ; ASSAY
英文摘要

The major clinically relevant species in Scedosporium (teleomorph Pseudallescheria) are Pseudallescheria boydii, Scedosporium aurantiacum, Scedosporium apiospermum, and Scedosporium prolificans, while Pseudallescheria minutispora, Petriellopsis desertorum, and Scedosporium dehoogii are exceptional agents of disease. Three molecular methods targeting the partial beta-tubulin gene were developed and evaluated to identify six closely related species of the S. apiospermum complex using quantitative real-time PCR (qPCR), PCR-based reverse line blot (PCR-RLB), and loop-mediated isothermal amplification (LAMP). qPCR was not specific enough for the identification of all species but had the highest sensitivity. The PCR-RLB assay was efficient for the identification of five species. LAMP distinguished all six species unambiguously. The analytical sensitivities of qPCR, PCR-RLB, and LAMP combined with MagNAPure, CTAB (cetyltrimethylammonium bromide), and FTA filter (Whatman) extraction were 50, 5 x 10(3), and 5 x 10(2) cells/mu l, respectively. When LAMP was combined with a simplified DNA extraction method using an FTA filter, identification to the species level was achieved within 2 h, including DNA extraction. The FTA-LAMP assay is therefore recommended as a cost-effective, simple, and rapid method for the identification of Scedosporium species.

语种英语
资助者Health Profession of China ; 11th Five-Year Key Special Subject for Science and Technical Research of China ; China Scholarship Council ; Health Profession of China ; 11th Five-Year Key Special Subject for Science and Technical Research of China ; China Scholarship Council
WOS记录号WOS:000287967100030
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60242
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Peking Univ, Res Ctr Med Mycol, Beijing 100034, Peoples R China
2.Centraalbur Schimmelcultures KNAW Fungal Biodiver, Utrecht, Netherlands
3.Fed Inst Drugs & Med Devices BfArM, Biosafety Lab, Bonn, Germany
4.Univ Innsbruck, Inst Microbiol, A-6020 Innsbruck, Austria
5.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
6.Radboud Univ Nijmegen, Med Ctr, NL-6525 ED Nijmegen, Netherlands
7.Sun Yat Sen Univ Guangzhou, Zhongshan Sch Med, Dept Parasitol, Guangzhou, Guangdong, Peoples R China
8.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
9.Mashhad Univ Med Sci, Dept Parasitol & Mycol, Avicenna Bu 16 Ali Res Inst, Mashhad, Iran
推荐引用方式
GB/T 7714
Lu, Qiaoyun,van den Ende, A. H. G. Gerrits,Bakkers, J. M. J. E.,et al. Identification of Pseudallescheria and Scedosporium Species by Three Molecular Methods[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2011,49(3):960-967.
APA Lu, Qiaoyun.,van den Ende, A. H. G. Gerrits.,Bakkers, J. M. J. E..,Sun, Jiufeng.,Lackner, M..,...&de Hoog, G. S..(2011).Identification of Pseudallescheria and Scedosporium Species by Three Molecular Methods.JOURNAL OF CLINICAL MICROBIOLOGY,49(3),960-967.
MLA Lu, Qiaoyun,et al."Identification of Pseudallescheria and Scedosporium Species by Three Molecular Methods".JOURNAL OF CLINICAL MICROBIOLOGY 49.3(2011):960-967.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lu, Qiaoyun]的文章
[van den Ende, A. H. G. Gerrits]的文章
[Bakkers, J. M. J. E.]的文章
百度学术
百度学术中相似的文章
[Lu, Qiaoyun]的文章
[van den Ende, A. H. G. Gerrits]的文章
[Bakkers, J. M. J. E.]的文章
必应学术
必应学术中相似的文章
[Lu, Qiaoyun]的文章
[van den Ende, A. H. G. Gerrits]的文章
[Bakkers, J. M. J. E.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。