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学科主题: 药学
题名:
Sequence analysis of Chinese and Japanese Curcuma drugs on the 18S rRNA gene and trnK gene and the application of amplification-refractory mutation system analysis for their authentication
作者: Sasaki, Y; Fushimi, H; Cao, H; Cai, SQ; Komatsu, K
关键词: Curcuma ; 18S rRNA gene ; trnK gene ; authentication ; amplification-refractory mutation system ; molecular taxonomy
刊名: BIOLOGICAL & PHARMACEUTICAL BULLETIN
发表日期: 2002-12-01
卷: 25, 期:12, 页:1593-1599
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
英文摘要:

The botanical origins of Chinese and Japanese Curcuma drugs were determined to be Curcuma longa, C. phaeocaulis, the Japanese population of C. zedoaria, C kwangsiensis, C. wenyujin, and C. aromatica based on a comparison of their 18S rRNA gene and trnK gene sequences with those of six Curcuma species reported previously. Moreover, to develop a more convenient identification method, amplification-refractory mutation system (ARMS) analysis of both gene regions was performed on plants. The ARMS method for the 18S rRNA gene was established using two types of forward primers designed based on the nucleotide difference at position 234. When DNAs of four Curcuma species were used as templates, PCR amplification with either of the two primers only generated a fragment of 912 base pairs (bp). However, when DNAs, of the purple-cloud type of C. kwangsiensis and C wenyujin were used, PCR amplifications with both primers unexpectedly generated the fragment, suggesting that these two were heterozygotes. The ARMS method for the trnK gene was also established using a mixture of four types of specific reverse primers designed on the basis of base substitutions and indels among six species, and common reverse and forward primers. C. phaeocaulis or the Chinese population of C. zedoaria, the Japanese population of C. zedoaria or the purple-cloud type of C. kwangsiensis, the pubescent type of C. kwangsiensis or C. wenyujin, and C. aromatica were found to show specific fragments of 730, 185, 527 or 528, and 641 or 642 bp, respectively. All species including C. longa also showed a common fragment of 897-904 bp. Using both ARMS methods, together with information on producing areas, the identification of Curcuma plants was achieved. Moreover, the ARMS method for the trnK gene was also useful for authentication of Curcuma drugs.

语种: 英语
WOS记录号: WOS:000179590100016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60260
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Toyama Med & Pharmaceut Univ, Inst Nat Med, Res Ctr Ethnomed, Toyama 9300194, Japan
2.Chinese Acad Tradit Chinese Med, Inst Chinese Mat Med, Beijing 107000, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China

Recommended Citation:
Sasaki, Y,Fushimi, H,Cao, H,et al. Sequence analysis of Chinese and Japanese Curcuma drugs on the 18S rRNA gene and trnK gene and the application of amplification-refractory mutation system analysis for their authentication[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2002,25(12):1593-1599.
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