IR@PKUHSC  > 北京大学第二临床医学院  > 肝胆外科
学科主题临床医学
Cyclosporine Combined With Nonlytic Interleukin 2/Fc Fusion Protein Improves Immune Response to Hepatitis B Vaccination in a Mouse Skin Transplantation Model
Li, Z.1; Zhang, D.2; Gao, J.1; Li, W-X.1; Zhu, J-Y.1
刊名TRANSPLANTATION PROCEEDINGS
2013-07-01
DOI10.1016/j.transproceed.2013.02.123
45期:6页:2559-2564
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Surgery ; Transplantation
研究领域[WOS]Immunology ; Surgery ; Transplantation
关键词[WOS]FOLLICULAR-HELPER-CELLS ; LIVER-TRANSPLANTATION ; SURFACE-ANTIGEN ; IN-VITRO ; T-CELLS ; TOLERANCE ; IL-2 ; DIFFERENTIATION ; FAILURE
英文摘要

Background. Improving immune responses to vaccination in immunosuppressed patients is extremely important. Previously, we observed that cyclosporine (CsA) combined with a nonlytic interleukin (IL)-2/fragment crystallizable (Fc) fusion protein induces immune tolerance to mouse skin transplantations. In the present study, we asked whether this combination improved hepatitis B (HBV) vaccine efficacy in immunosuppressed mice while also prolonging skin graft survival.

Methods. After C57BL/6 mice received DBA/2 skin grafts, they were administered a 14-day course of CsA (30 mg/kg intraperitoneal) combined with IL-2/Fc (1 mu g, intraperitoneal). HBV vaccine (2 mu g) was injected intramuscularly on the day of skin transplantation. On day 14, the serum levels of hepatitis B surface antibody (HBsAb), IL-4, IL-10, interferon (IFN-gamma), and IL-2 were measured by enzyme-linked immunosorbent assay. We assessed the percentages of CD4(+)CXCR5(+) follicular T helper cells, CD4(+)FoxP3(+) regulatory T cells (Treg) and expressions of IL-17, IL-21, FoxP3, Bcl-6 in the spleen. Animals were divided into four groups: control, vaccine-treated, CsA vaccine-treated, CsA + IL-2/Fc + vaccine-treated hosts.

Results. Combination therapy significantly increased HBsAb levels and also prolonged skin graft survival. Serum levels of Th1 cytokines IL-2 and IFN-gamma were significantly higher in the combination group, while Th2 cytokines, IL-4 and IL-10 were lower. Combined treatment increased the percentage of Treg and the expression of Foxp3 and IL-21, meanwhile inhibiting the expression of Bcl-6. But the percentage of Tfh did not significantly change among the groups.

Conclusions. These observations suggested that a combination of CsA and IL-2/Fc fusion protein enhanced immune responses after HBV vaccination and prolonged skin graft survival.

语种英语
WOS记录号WOS:000323852000087
项目编号2009CB522403
资助机构National Basic Research Program of China (973 Program)
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60263
专题北京大学第二临床医学院_肝胆外科
作者单位1.Peking Univ, Ctr Transplantat, Peoples Hosp, Dept Hepatobiliary Surg, Beijing 100044, Peoples R China
2.Beijing Friendship Hosp, Dept Liver Dis Inst, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Li, Z.,Zhang, D.,Gao, J.,et al. Cyclosporine Combined With Nonlytic Interleukin 2/Fc Fusion Protein Improves Immune Response to Hepatitis B Vaccination in a Mouse Skin Transplantation Model[J]. TRANSPLANTATION PROCEEDINGS,2013,45(6):2559-2564.
APA Li, Z.,Zhang, D.,Gao, J.,Li, W-X.,&Zhu, J-Y..(2013).Cyclosporine Combined With Nonlytic Interleukin 2/Fc Fusion Protein Improves Immune Response to Hepatitis B Vaccination in a Mouse Skin Transplantation Model.TRANSPLANTATION PROCEEDINGS,45(6),2559-2564.
MLA Li, Z.,et al."Cyclosporine Combined With Nonlytic Interleukin 2/Fc Fusion Protein Improves Immune Response to Hepatitis B Vaccination in a Mouse Skin Transplantation Model".TRANSPLANTATION PROCEEDINGS 45.6(2013):2559-2564.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Z.]的文章
[Zhang, D.]的文章
[Gao, J.]的文章
百度学术
百度学术中相似的文章
[Li, Z.]的文章
[Zhang, D.]的文章
[Gao, J.]的文章
必应学术
必应学术中相似的文章
[Li, Z.]的文章
[Zhang, D.]的文章
[Gao, J.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。