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学科主题临床医学
Determination of Tumor-infiltrating CD8(+) Lymphocytes in Human Ovarian Cancer
Aust, Stefanie1; Bachmayr-Heyda, Anna1; Pils, Dietmar1; Zhao, Lijun5; Tong, Weimin6,7,8; Berger, Astrid4; Fogel, Mina9; Thalhammer, Theresa2; Sehouli, Jalid10; Horvat, Reinhard3; Zeillinger, Robert1; Castillo-Tong, Dan C.1
关键词Tissue microarray Whole-tissue section Tumor-infiltrating cells CD8(+) cells Ovarian cancer
刊名INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY
2013-05-01
DOI10.1097/PGP.0b013e31826a63f8
32期:3页:269-276
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Pathology
资助者European Union (EU) ; European Union (EU)
研究领域[WOS]Obstetrics & Gynecology ; Pathology
关键词[WOS]TISSUE MICROARRAY TECHNOLOGY ; T-CELL RATIO ; IMMUNOHISTOCHEMICAL MARKERS ; LUNG-CANCER ; VALIDATION ; QUANTIFICATION ; PROGNOSIS ; CARCINOMA ; SPECIMENS ; SURVIVAL
英文摘要

Tumor-infiltrating immune cells and their prognostic value have been analyzed in various malignancies. Although tissue microarray (TMA) has been used in some of these studies, it is still questionable whether this technique can represent the results of infiltrating CD8(+) cells obtained from whole-tissue sections (WTS). The aim of this study was to assess and compare the density of tumor-infiltrating CD8(+) cells in ovarian cancer using TMA and WTS. CD8(+) lymphocytes were immunohistochemically stained on WTS and TMA cores from 37 ovarian cancer patients and quantified using the image analysis software HistoQuest. Four different areas were selected on the WTS, namely (i) tumor; (ii) stroma; (iii) mixed; and (iv) dense, whereby dense represented areas of most abundant CD8(+) cells. On the TMA, (i) the whole TMA cores and (ii) areas containing only epithelial tumor tissue were analyzed. The Pearson correlation and principal component analysis was used to estimate the correlation of results from different techniques. CD8(+) lymphocytes showed highly correlated measurements between tumor, mixed, and dense areas. Moderate correlations were found between each of these 3 measurements and stroma. CD8(+) cell counts from WTS showed moderate correlation with TMA cell counts. Consistently, principal component analysis showed 3 clusters (i) tumor, dense, mixed; (ii) stroma; and (iii) TMA areas. Taken together, when the prognostic impact of tumor-infiltrating CD8(+) cells in ovarian cancer is investigated with TMA technique, a moderate correlation with WTS results has to be considered.

语种英语
所属项目编号018698
资助者European Union (EU) ; European Union (EU)
WOS记录号WOS:000317482300003
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60266
Collection北京大学第二临床医学院_妇科
作者单位1.Med Univ Vienna, Clin Inst Pathol, A-1090 Vienna, Austria
2.Kaplan Med Ctr, Dept Pathol, Rehovot, Israel
3.Med Univ Vienna, Mol Oncol Grp, Dept Obstet & Gynecol, A-1090 Vienna, Austria
4.Med Univ Vienna, Ctr Pathophysiol & Allergy Res, Dept Pathophysiol, A-1090 Vienna, Austria
5.Med Univ Innsbruck, Dept Obstet & Gynecol, A-6020 Innsbruck, Austria
6.Peking Univ, Peoples Hosp, Dept Gynecol, Beijing 100871, Peoples R China
7.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Pathol, Beijing 100730, Peoples R China
8.Chinese Acad Med Sci, Inst Basic Med Sci, Ctr Expt Anim Res, Beijing 100730, Peoples R China
9.Peking Union Med Coll, Beijing 100021, Peoples R China
10.Charite, European Competence Ctr Ovarian Canc, Dept Gynecol, Berlin, Germany
Recommended Citation
GB/T 7714
Aust, Stefanie,Bachmayr-Heyda, Anna,Pils, Dietmar,et al. Determination of Tumor-infiltrating CD8(+) Lymphocytes in Human Ovarian Cancer[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY,2013,32(3):269-276.
APA Aust, Stefanie.,Bachmayr-Heyda, Anna.,Pils, Dietmar.,Zhao, Lijun.,Tong, Weimin.,...&Castillo-Tong, Dan C..(2013).Determination of Tumor-infiltrating CD8(+) Lymphocytes in Human Ovarian Cancer.INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY,32(3),269-276.
MLA Aust, Stefanie,et al."Determination of Tumor-infiltrating CD8(+) Lymphocytes in Human Ovarian Cancer".INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY 32.3(2013):269-276.
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