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学科主题: 临床医学
题名:
Determination of Tumor-infiltrating CD8(+) Lymphocytes in Human Ovarian Cancer
作者: Aust, Stefanie1; Bachmayr-Heyda, Anna1; Pils, Dietmar1; Zhao, Lijun5; Tong, Weimin6,7,8; Berger, Astrid4; Fogel, Mina9; Thalhammer, Theresa2; Sehouli, Jalid10; Horvat, Reinhard3; Zeillinger, Robert1; Castillo-Tong, Dan C.1
关键词: Tissue microarray ; Whole-tissue section ; Tumor-infiltrating cells ; CD8(+) cells ; Ovarian cancer
刊名: INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY
发表日期: 2013-05-01
DOI: 10.1097/PGP.0b013e31826a63f8
卷: 32, 期:3, 页:269-276
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Pathology
研究领域[WOS]: Obstetrics & Gynecology ; Pathology
关键词[WOS]: TISSUE MICROARRAY TECHNOLOGY ; T-CELL RATIO ; IMMUNOHISTOCHEMICAL MARKERS ; LUNG-CANCER ; VALIDATION ; QUANTIFICATION ; PROGNOSIS ; CARCINOMA ; SPECIMENS ; SURVIVAL
英文摘要:

Tumor-infiltrating immune cells and their prognostic value have been analyzed in various malignancies. Although tissue microarray (TMA) has been used in some of these studies, it is still questionable whether this technique can represent the results of infiltrating CD8(+) cells obtained from whole-tissue sections (WTS). The aim of this study was to assess and compare the density of tumor-infiltrating CD8(+) cells in ovarian cancer using TMA and WTS. CD8(+) lymphocytes were immunohistochemically stained on WTS and TMA cores from 37 ovarian cancer patients and quantified using the image analysis software HistoQuest. Four different areas were selected on the WTS, namely (i) tumor; (ii) stroma; (iii) mixed; and (iv) dense, whereby dense represented areas of most abundant CD8(+) cells. On the TMA, (i) the whole TMA cores and (ii) areas containing only epithelial tumor tissue were analyzed. The Pearson correlation and principal component analysis was used to estimate the correlation of results from different techniques. CD8(+) lymphocytes showed highly correlated measurements between tumor, mixed, and dense areas. Moderate correlations were found between each of these 3 measurements and stroma. CD8(+) cell counts from WTS showed moderate correlation with TMA cell counts. Consistently, principal component analysis showed 3 clusters (i) tumor, dense, mixed; (ii) stroma; and (iii) TMA areas. Taken together, when the prognostic impact of tumor-infiltrating CD8(+) cells in ovarian cancer is investigated with TMA technique, a moderate correlation with WTS results has to be considered.

语种: 英语
所属项目编号: 018698
项目资助者: European Union (EU)
WOS记录号: WOS:000317482300003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60266
Appears in Collections:北京大学第二临床医学院_妇科_期刊论文

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作者单位: 1.Med Univ Vienna, Clin Inst Pathol, A-1090 Vienna, Austria
2.Kaplan Med Ctr, Dept Pathol, Rehovot, Israel
3.Med Univ Vienna, Mol Oncol Grp, Dept Obstet & Gynecol, A-1090 Vienna, Austria
4.Med Univ Vienna, Ctr Pathophysiol & Allergy Res, Dept Pathophysiol, A-1090 Vienna, Austria
5.Med Univ Innsbruck, Dept Obstet & Gynecol, A-6020 Innsbruck, Austria
6.Peking Univ, Peoples Hosp, Dept Gynecol, Beijing 100871, Peoples R China
7.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Pathol, Beijing 100730, Peoples R China
8.Chinese Acad Med Sci, Inst Basic Med Sci, Ctr Expt Anim Res, Beijing 100730, Peoples R China
9.Peking Union Med Coll, Beijing 100021, Peoples R China
10.Charite, European Competence Ctr Ovarian Canc, Dept Gynecol, Berlin, Germany

Recommended Citation:
Aust, Stefanie,Bachmayr-Heyda, Anna,Pils, Dietmar,et al. Determination of Tumor-infiltrating CD8(+) Lymphocytes in Human Ovarian Cancer[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY,2013,32(3):269-276.
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