北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 儿科  > 期刊论文
学科主题: 临床医学
题名:
Immune regulation of hydrogen sulfide in children with acute lymphoblastic leukemia
作者: Du Shuxu1,3; Jia Yongrui2; Tang Hong1; Sun Yangling1; Wu Wanshui1; Sun Liming1; Du Junbao; Geng Bin4; Tang Chaoshu4; Jin Hongfang3
关键词: child ; acute lymphoblastic leukemia ; hydrogen sulfide ; cytokine ; immune
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2014-11-05
DOI: 10.3760/cma.j.issn.0366-6999.20133106
卷: 127, 期:21, 页:3695-3699
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: CENTRAL-NERVOUS-SYSTEM ; CELLS ; EXPRESSION ; RISK ; CYTOKINES ; KINASE ; GROWTH ; CANCER
英文摘要:

Background Acute lymphoblastic leukemia (ALL) and chemotherapy can cause immune imbalance, and gaseous molecule hydrogen sulfide (H2S) can participate in the process of immune response. This study aimed to investigate the immune regulation of H2S in pediatric ALL.

Methods Children (n=78) with ALL admitted during 2010-2013 were included in this study. Two blood samples were collected in period of before chemotherapy, bone marrow remission and two days after chemotherapy, respectively. Serum contents of H2S and cytokines, including interleukin-1 beta (IL-1 beta), interleukin-2 (IL-2), interferon-gamma (IFN-gamma), tumor necrosis factor (TNF-alpha), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10) and macrophage inflammatory protein-1 alpha (MIP-1 alpha), were detected using ELISA method. Stepwise regression was used to analyze the correlation between H2S and cytokines. Furthermore, human Jurkat cells were cultured in vitro, and nucleoprotein of Jurkat cells and peripheral blood mononuclear cells (PBMCs) were collected, contents of cystathionine gamma-lyase (CSE) and certain cytokines were measured by Western blotting.

Results Serum concentrations of H2S, IL-1 beta, IL-6, IL-10 and MIP-1 alpha in children with ALL were increased significantly (P <0.01), while concentrations of IL-2, TNF-alpha, IFN-gamma and IL-4 decreased obviously (P <0.01). In patients after chemotherapy, concentrations of H2S and IL-10 were decreased significantly (P <0.05), but IL-4 and IFN-gamma concentrations increased markedly (P <0.05). At remission stage, H2S, IL-1 beta, IL-4, IL-6, IL-10 and MIP-1 alpha concentrations were further decreased markedly (P <0.05), but concentrations of IL-2, TNF-alpha and IFN-gamma increased again (P <0.05). Protein contents of CSE, IL-10, IL-4 and IL-2 of PBMCs also increased markedly in children with ALL. Moreover, changes of CSE protein contents of PBMCs were consistent with serum H2S contents, and there were significant correlation between H(2)5 and certain cytokines based on stepwise regression analysis. Furthermore, compared with those of PBMCs group, in vitro study indicated that Jurkat cells of H2S group expressed IFN-gamma, IL-10, IL-4 and IL-2 protein increased obviously (P <0.05), while IL-4, IL-2 and CSE expression of PPG group decreased markedly (P <0.05).

Conclusion Gaseous molecule H2S might participate in the process of immune regulation in pediatric ALL through modulating transcription and expression of cytokines.

语种: 英语
所属项目编号: 2013CB933801 ; 2012D003034000004
项目资助者: Major Basic Research Program of China ; Talents Funded Class D Projects of Beijing
WOS记录号: WOS:000346329300004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60267
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Capital Med Univ, Shijitan Hosp, Dept Pediat, Beijing 100038, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China

Recommended Citation:
Du Shuxu,Jia Yongrui,Tang Hong,et al. Immune regulation of hydrogen sulfide in children with acute lymphoblastic leukemia[J]. CHINESE MEDICAL JOURNAL,2014,127(21):3695-3699.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Du Shuxu]'s Articles
[Jia Yongrui]'s Articles
[Tang Hong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Du Shuxu]‘s Articles
[Jia Yongrui]‘s Articles
[Tang Hong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace