IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Adjuvanticity of a Recombinant Calreticulin Fragment in Assisting Anti-beta-Glucan IgG Responses in T Cell-Deficient Mice
Li, Wei-Ji1; Long, Kai1; Dong, Hong-Liang2; Gao, Xiao-Ming1,2
刊名CLINICAL AND VACCINE IMMUNOLOGY
2013-04-01
DOI10.1128/CVI.00689-12
20期:4页:582-589
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Infectious Diseases ; Microbiology
资助者PCSIRT ; National Foundation of Natural Science of China ; National Key Basic Research Programs ; Priority Academic Program Development of Jiangsu Province Higher Education Institutions ; PCSIRT ; National Foundation of Natural Science of China ; National Key Basic Research Programs ; Priority Academic Program Development of Jiangsu Province Higher Education Institutions
研究领域[WOS]Immunology ; Infectious Diseases ; Microbiology
关键词[WOS]HEAT-SHOCK PROTEINS ; CHIMERIC MOLECULAR ADJUVANT ; CONJUGATE VACCINE ; CANDIDA-ALBICANS ; MONOCLONAL-ANTIBODY ; IMMUNE-RESPONSE ; PROTECTION ; RECEPTOR ; DISEASE ; SURFACE
英文摘要

Polysaccharide-encapsulated fungi are the chief source of diseases in immunocompromised hosts such as those infected with human immunodeficiency virus or neutropenia patients. Currently available polysaccharide-protein conjugate vaccines are mainly T cell dependent and are usually ineffective in weakened immune systems. In this study, laminarin, a well-characterized beta-1,3-glucan, was conjugated with a prokaryotically expressed recombinant fragment (amino acids [aa] 39 to 272) of calreticulin (rCRT/39-272), which exhibits extraordinarily potent immunogenicity and adjuvanticity in experimental animals. The resultant conjugate reserves the immunostimulatory effect of rCRT/39-272 on naive murine B cells and is capable of eliciting anti-beta-glucan IgG (mostly IgG1) responses in not only BALB/c mice but also athymic nude mice. Laminarin-CRT-induced mouse antibodies (Abs) are able to bind with Candida albicans and inhibit its growth in vitro. In addition, vaccination with laminarin-CRT partially protects mice from lethal C. albicans challenge. These results imply that rCRT/39-272 could be used as an ideal carrier or adjuvant for carbohydrate vaccines aimed at inducing or boosting IgG responses to fungal infections in immunodeficient hosts.

语种英语
所属项目编号IRT1075 ; 30890142 ; 2010CB529102
资助者PCSIRT ; National Foundation of Natural Science of China ; National Key Basic Research Programs ; Priority Academic Program Development of Jiangsu Province Higher Education Institutions ; PCSIRT ; National Foundation of Natural Science of China ; National Key Basic Research Programs ; Priority Academic Program Development of Jiangsu Province Higher Education Institutions
WOS记录号WOS:000316662400019
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60273
Collection北京大学基础医学院_免疫学系
作者单位1.Soochow Univ, Inst Biol & Med Sci, Suzhou, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Li, Wei-Ji,Long, Kai,Dong, Hong-Liang,et al. Adjuvanticity of a Recombinant Calreticulin Fragment in Assisting Anti-beta-Glucan IgG Responses in T Cell-Deficient Mice[J]. CLINICAL AND VACCINE IMMUNOLOGY,2013,20(4):582-589.
APA Li, Wei-Ji,Long, Kai,Dong, Hong-Liang,&Gao, Xiao-Ming.(2013).Adjuvanticity of a Recombinant Calreticulin Fragment in Assisting Anti-beta-Glucan IgG Responses in T Cell-Deficient Mice.CLINICAL AND VACCINE IMMUNOLOGY,20(4),582-589.
MLA Li, Wei-Ji,et al."Adjuvanticity of a Recombinant Calreticulin Fragment in Assisting Anti-beta-Glucan IgG Responses in T Cell-Deficient Mice".CLINICAL AND VACCINE IMMUNOLOGY 20.4(2013):582-589.
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