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Effect of Hydroxysafflor yellow A on human umbilical vein endothelial cells under hypoxia
Ji, Deng Bo1,2,3; Zhang, Li Yun4; Li, Chang Ling1; Ye, Jia1; Zhu, Hai Bo2,3
关键词Hydroxysafflor yellow A Human umbilical vein endothelial cells Hypoxia
刊名VASCULAR PHARMACOLOGY
2009-03-01
DOI10.1016/j.vph.2008.11.009
50期:3-4页:137-145
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]PERMEABILITY TRANSITION PORE ; NITRIC-OXIDE ; APOPTOSIS ; P53 ; DEFICIENT ; PATHWAYS ; INJURY ; MICE
英文摘要

Hydroxysafflor yellow A (HSYA), is a component of the flower, Carthamus tinctorius L In this study, we investigated its effect on Human Umbilical Vein Endothelial Cells (HUVECs) under hypoxia. We evaluated cell viability using the MTT kit. The cell cycle distribution was analyzed by PI staining flow cytometric analysis. PI AnnexinV-FITC detection and the TUNEL assay were performed to evaluate the apoptosis rate. Nitric oxide (NO) generation in cell supernatant was measured by the Griess assay. RT-PCR, Western blot and Immunocytochemistry analysis were used to evaluate the changes of Bcl-2, Bax, p53 and eNOS. Our data showed that HSYA inhibited cell apoptosis and cell cycle G1 arrest induced by hypoxia. HSYA treatment increased the Bcl-2/Bax ratio of protein and mRNA, reduced p53 protein expression in cell nucleus. In addition, HSYA enhanced the NO content of cell supernatant under hypoxia, accompanied with upregulating eNOS mRNA expression and protein level. Taken together, these results demonstrate that HSYA could protect HUVECs from hypoxia induced injuries by inhibiting cell apoptosis and cell cycle arrest. These findings have partly revealed the molecular mechanism of HSYA on treating of ischemic heart disease. We expected our experiments might provide some clues for further research. (c) 2008 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000263152100009
项目编号30370720,30572343 ; 2004CB518906 ; IRT0514
资助机构National Natural Sciences Foundation of China (NSFC) ; National 973 Fundamental Project of China ; Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)
引用统计
被引频次:40[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60281
专题北京大学药学院
北京大学药学院_分子与细胞药理学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China
2.Chinese Acad Med Sci, Inst Mat Med, Minist Educ, Key Lab Bioact Subst & Resources Utilizat Chinese, Beijing 100050, Peoples R China
3.Peking Union Med Coll, Beijing 100050, Peoples R China
4.First Gen Hosp N China Petr, Dept Cardiol, Langfang 065007, Peoples R China
推荐引用方式
GB/T 7714
Ji, Deng Bo,Zhang, Li Yun,Li, Chang Ling,et al. Effect of Hydroxysafflor yellow A on human umbilical vein endothelial cells under hypoxia[J]. VASCULAR PHARMACOLOGY,2009,50(3-4):137-145.
APA Ji, Deng Bo,Zhang, Li Yun,Li, Chang Ling,Ye, Jia,&Zhu, Hai Bo.(2009).Effect of Hydroxysafflor yellow A on human umbilical vein endothelial cells under hypoxia.VASCULAR PHARMACOLOGY,50(3-4),137-145.
MLA Ji, Deng Bo,et al."Effect of Hydroxysafflor yellow A on human umbilical vein endothelial cells under hypoxia".VASCULAR PHARMACOLOGY 50.3-4(2009):137-145.
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