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学科主题: 药学
题名:
Effect of Hydroxysafflor yellow A on human umbilical vein endothelial cells under hypoxia
作者: Ji, Deng Bo1,2,3; Zhang, Li Yun4; Li, Chang Ling1; Ye, Jia1; Zhu, Hai Bo2,3
关键词: Hydroxysafflor yellow A ; Human umbilical vein endothelial cells ; Hypoxia
刊名: VASCULAR PHARMACOLOGY
发表日期: 2009-03-01
DOI: 10.1016/j.vph.2008.11.009
卷: 50, 期:3-4, 页:137-145
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: PERMEABILITY TRANSITION PORE ; NITRIC-OXIDE ; APOPTOSIS ; P53 ; DEFICIENT ; PATHWAYS ; INJURY ; MICE
英文摘要:

Hydroxysafflor yellow A (HSYA), is a component of the flower, Carthamus tinctorius L In this study, we investigated its effect on Human Umbilical Vein Endothelial Cells (HUVECs) under hypoxia. We evaluated cell viability using the MTT kit. The cell cycle distribution was analyzed by PI staining flow cytometric analysis. PI AnnexinV-FITC detection and the TUNEL assay were performed to evaluate the apoptosis rate. Nitric oxide (NO) generation in cell supernatant was measured by the Griess assay. RT-PCR, Western blot and Immunocytochemistry analysis were used to evaluate the changes of Bcl-2, Bax, p53 and eNOS. Our data showed that HSYA inhibited cell apoptosis and cell cycle G1 arrest induced by hypoxia. HSYA treatment increased the Bcl-2/Bax ratio of protein and mRNA, reduced p53 protein expression in cell nucleus. In addition, HSYA enhanced the NO content of cell supernatant under hypoxia, accompanied with upregulating eNOS mRNA expression and protein level. Taken together, these results demonstrate that HSYA could protect HUVECs from hypoxia induced injuries by inhibiting cell apoptosis and cell cycle arrest. These findings have partly revealed the molecular mechanism of HSYA on treating of ischemic heart disease. We expected our experiments might provide some clues for further research. (c) 2008 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30370720,30572343 ; 2004CB518906 ; IRT0514
项目资助者: National Natural Sciences Foundation of China (NSFC) ; National 973 Fundamental Project of China ; Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)
WOS记录号: WOS:000263152100009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60281
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China
2.Chinese Acad Med Sci, Inst Mat Med, Minist Educ, Key Lab Bioact Subst & Resources Utilizat Chinese, Beijing 100050, Peoples R China
3.Peking Union Med Coll, Beijing 100050, Peoples R China
4.First Gen Hosp N China Petr, Dept Cardiol, Langfang 065007, Peoples R China

Recommended Citation:
Ji, Deng Bo,Zhang, Li Yun,Li, Chang Ling,et al. Effect of Hydroxysafflor yellow A on human umbilical vein endothelial cells under hypoxia[J]. VASCULAR PHARMACOLOGY,2009,50(3-4):137-145.
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