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学科主题: 临床医学
题名:
Activation of nuclear factor-kappa B during retinal degeneration in rd Mice
作者: Zeng, Hui-yang1,2; Tso, Mark O. M.1,2; Lai, Shenghan3; Lai, Hong1
刊名: MOLECULAR VISION
发表日期: 2008-06-10
卷: 14, 期:128, 页:1075-1080
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Ophthalmology
研究领域[WOS]: Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]: AMYLOID BETA-PEPTIDE ; HIPPOCAMPAL-NEURONS ; RETINITIS-PIGMENTOSA ; TRANSCRIPTION FACTOR ; ALZHEIMERS-DISEASE ; INDUCED APOPTOSIS ; MOUSE MODEL ; EXPRESSION ; MICROGLIA ; GENE
英文摘要:

Purpose: Transcription factors of the nuclear factor-kappa beta (NF-kappa B) family have been demonstrated to play an important role in the regulation of gene expression in the chronic neurodegenerative disorders. The aims of the current study were to investigate the alteration of NF-kappa B activity during retinal degeneration in rd mice and further explore its role in photoreceptor apoptosis.

Methods: Activation of NF-kappa B and its nuclear translocation in the retina of rd mice at postnatal days (P) 8, 10, 12, 14, 16, 18, and 28 were studied by immunohistochemical analysis using NF-kappa B P65 antibody. The amount of NF-kappa B P65 protein and NF-kappa B DNA-binding activity in the whole retina were assessed by western blot analysis and gel shift analysis, respectively. Expression of NF-kappa B in microglial cells labeled with CD11b was determined by double labeling.

Results: NF-kappa B P65 nuclear translocation and its DNA binding activity started to increase in the rd retina at P10 and reached a peak at P12. Expressions of P65 remained at high levels from P12 to P18. Double labeling of P65 with CD11 at P14 showed colocalization of P65 in the microglial cells in the outer nuclear layer.

Conclusions: NF-kappa B was activated in the retinal degeneration of rd mice. NF-kappa B modulation may play a role in the retinal degeneration through microglial activation.

语种: 英语
WOS记录号: WOS:000257750300001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60295
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: 1.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21205 USA
2.Peking Univ, Hosp 3, Ctr Eye, Beijing 100871, Peoples R China
3.Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA

Recommended Citation:
Zeng, Hui-yang,Tso, Mark O. M.,Lai, Shenghan,et al. Activation of nuclear factor-kappa B during retinal degeneration in rd Mice[J]. MOLECULAR VISION,2008,14(128):1075-1080.
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