IR@PKUHSC  > 北京大学第二临床医学院  > 神经外科
学科主题临床医学
Trichosanthin suppresses the proliferation of glioma cells by inhibiting LGR5 expression and the Wnt/beta-catenin signaling pathway
Mia, Junjie; Jiang, Yilin; Wang, Dongliang; Zhou, Jingru; Fan, Cungang; Jiao, Feng; Liu, Bo; Zhang, Jun; Wang, Yangshuo; Zhang, Qingjun
关键词trichosanthin glioma apoptosis LGR5 Wnt/beta-catenin signaling
刊名ONCOLOGY REPORTS
2015-12-01
DOI10.3892/or.2015.4290
34期:6页:2845-2852
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]RIBOSOME-INACTIVATING PROTEIN ; RICIN-A-CHAIN ; INDUCED APOPTOSIS ; MEDIATED APOPTOSIS ; COUPLED RECEPTORS ; CANCER CELLS ; STEM-CELLS ; IN-VITRO ; IDENTIFICATION ; MECHANISM
英文摘要

Studies have indicated that trichosanthin (TCS), a bioactive protein extracted and purified from the tuberous root of Trichosanthes kirilowii (a well-known traditional Chinese medicinal plant), produces antitumor effects on various types of cancer cells. However, the effects of TCS on glioma cells are poorly understood. The objective of this study was to investigate the antitumor effects of TCS on the U87 and U251 cell lines. The in vitro effects of TCS on these two cell lines were determined using a Cell Counting Kit-8 (CCK-8) assay, Annexin V-FITC staining, DAPI staining, Transwell assays, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assays, 5,5,6,61-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1) staining and western blotting, which was utilized to assess the expression of leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) and key proteins in the Wnt/beta-catenin signaling pathway. Our data indicated that TCS inhibited the proliferation of glioma cells in a dose- and time-dependent manner and played a role in inhibiting glioma cell invasion and migration. Additional investigation revealed that the expression levels of LGR5 and of key proteins in the Wnt/beta-catenin signaling pathway were markedly decreased after TCS treatment-The results suggest that TCS may induce apoptosis in glioma cells by targeting LGR5 and repressing the Wnt/beta-catenin signaling pathway. In the future, in vivo experiments should be conducted to examine the potential use of this compound as a novel therapeutic agent for gliomas.

语种英语
WOS记录号WOS:000364617000005
项目编号RDB2011-14 ; 81001009
资助机构Peking University People&prime ; s Hospital Research and Development Funds ; National Natural Science Foundation of China
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60300
专题北京大学第二临床医学院_神经外科
作者单位Peking Univ, Peoples Hosp, Dept Neurosurg, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Mia, Junjie,Jiang, Yilin,Wang, Dongliang,et al. Trichosanthin suppresses the proliferation of glioma cells by inhibiting LGR5 expression and the Wnt/beta-catenin signaling pathway[J]. ONCOLOGY REPORTS,2015,34(6):2845-2852.
APA Mia, Junjie.,Jiang, Yilin.,Wang, Dongliang.,Zhou, Jingru.,Fan, Cungang.,...&Zhang, Qingjun.(2015).Trichosanthin suppresses the proliferation of glioma cells by inhibiting LGR5 expression and the Wnt/beta-catenin signaling pathway.ONCOLOGY REPORTS,34(6),2845-2852.
MLA Mia, Junjie,et al."Trichosanthin suppresses the proliferation of glioma cells by inhibiting LGR5 expression and the Wnt/beta-catenin signaling pathway".ONCOLOGY REPORTS 34.6(2015):2845-2852.
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