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学科主题: 临床医学
题名:
Trichosanthin suppresses the proliferation of glioma cells by inhibiting LGR5 expression and the Wnt/beta-catenin signaling pathway
作者: Mia, Junjie; Jiang, Yilin; Wang, Dongliang; Zhou, Jingru; Fan, Cungang; Jiao, Feng; Liu, Bo; Zhang, Jun; Wang, Yangshuo; Zhang, Qingjun
关键词: trichosanthin ; glioma ; apoptosis ; LGR5 ; Wnt/beta-catenin signaling
刊名: ONCOLOGY REPORTS
发表日期: 2015-12-01
DOI: 10.3892/or.2015.4290
卷: 34, 期:6, 页:2845-2852
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: RIBOSOME-INACTIVATING PROTEIN ; RICIN-A-CHAIN ; INDUCED APOPTOSIS ; MEDIATED APOPTOSIS ; COUPLED RECEPTORS ; CANCER CELLS ; STEM-CELLS ; IN-VITRO ; IDENTIFICATION ; MECHANISM
英文摘要:

Studies have indicated that trichosanthin (TCS), a bioactive protein extracted and purified from the tuberous root of Trichosanthes kirilowii (a well-known traditional Chinese medicinal plant), produces antitumor effects on various types of cancer cells. However, the effects of TCS on glioma cells are poorly understood. The objective of this study was to investigate the antitumor effects of TCS on the U87 and U251 cell lines. The in vitro effects of TCS on these two cell lines were determined using a Cell Counting Kit-8 (CCK-8) assay, Annexin V-FITC staining, DAPI staining, Transwell assays, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assays, 5,5,6,61-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1) staining and western blotting, which was utilized to assess the expression of leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) and key proteins in the Wnt/beta-catenin signaling pathway. Our data indicated that TCS inhibited the proliferation of glioma cells in a dose- and time-dependent manner and played a role in inhibiting glioma cell invasion and migration. Additional investigation revealed that the expression levels of LGR5 and of key proteins in the Wnt/beta-catenin signaling pathway were markedly decreased after TCS treatment-The results suggest that TCS may induce apoptosis in glioma cells by targeting LGR5 and repressing the Wnt/beta-catenin signaling pathway. In the future, in vivo experiments should be conducted to examine the potential use of this compound as a novel therapeutic agent for gliomas.

语种: 英语
所属项目编号: RDB2011-14 ; 81001009
项目资助者: Peking University People&prime ; s Hospital Research and Development Funds ; National Natural Science Foundation of China
WOS记录号: WOS:000364617000005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60300
Appears in Collections:北京大学第二临床医学院_神经外科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Neurosurg, Beijing 100044, Peoples R China

Recommended Citation:
Mia, Junjie,Jiang, Yilin,Wang, Dongliang,et al. Trichosanthin suppresses the proliferation of glioma cells by inhibiting LGR5 expression and the Wnt/beta-catenin signaling pathway[J]. ONCOLOGY REPORTS,2015,34(6):2845-2852.
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