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IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心  > 期刊论文
学科主题: 临床医学
题名:
Assessment of the Risk of Blastomere Biopsy during Preimplantation Genetic Diagnosis in a Mouse Model: Reducing Female Ovary Function with an Increase in Age by Proteomics Method
作者: Yu, Yang1,2,3; Zhao, Yue1,3; Li, Rong1,3; Li, Li1; Zhao, Hongcui1; Li, Min1; Sha, Jiahao4; Zhou, Qi5; Qiao, Jie1,2,3
关键词: preimplantation diagnosis ; embryo biopsy ; ovary function ; female fertility ; proteomics
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2013-12-01
DOI: 10.1021/pr400366j
卷: 12, 期:12, 页:5475-5486
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: MONOZYGOTIC TWINS ; PROTEASOME SUBUNIT ; BIRTH-DEFECTS ; EMBRYO BIOPSY ; PROTEIN ; MICE ; EXPRESSION ; FAILURE ; ANDROSTENEDIONE ; LIPODYSTROPHY
英文摘要:

Preimplantation genetic diagnosis (PGD) is important for screening genetic and chromosome mutations in embryos so that the efficiency of assisted reproductive treatment can be increased and birth defects can be decreased; however, some studies have reported a risk from this technology as well as other assisted reproductive technologies. We have developed a blastomere biopsy mouse model to assess the potential effects of blastomere biopsy that was one key procedure in PGD on the fertility of female mice at different ages. We showed that female fertility was decreased in the biopsied mouse model with an increase in age. Moreover, the ovarian weight, serum hormone levels, and the number of primordial, primary, preantral, and antral stage follicles were also decreased in the middle-aged biopsied mouse model. To elucidate the underlying molecular mechanism, we did proteomics analysis on ovarian tissues from puberty biopsied and nonbiopsied mice of the 23 differentially expressed proteins that were screened for in both groups, 3 proteins (PSMB8, ALDH1A1, and HSPA4) were selected and identified by Western blotting and quantitative RT-PCR methods, which showed the 3 proteins to regulate 12 cellular pathways. Furthermore, these three proteins were shown to be located in ovarian tissues, and the dynamic changes of expression profiling in middle-aged biopsied and nonbiopsied mice were demonstrated. The present study showed that blastomere biopsy technology impairs fertility when mice are middle-aged, which possibly resulted in abnormal expression profiling of PSMB8, ALDH1A1, and HSPA4 proteins. Thus, additional studies should be performed to assess the overall risk of blastomere biopsies during PGD procedures.

语种: 英语
所属项目编号: 2011CB944504 ; 2014CB943203 ; 31371521 ; 31000661
项目资助者: Ministry of Science and Technology of China (973 program) ; National Natural Science Funds for General Program ; National Natural Science Funds for Young Scholar
WOS记录号: WOS:000328231300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60313
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
4.Nanjing Med Univ, Dept Histol & Embryol, Lab Reprod Med, Nanjing 210029, Jiangsu, Peoples R China
5.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China

Recommended Citation:
Yu, Yang,Zhao, Yue,Li, Rong,et al. Assessment of the Risk of Blastomere Biopsy during Preimplantation Genetic Diagnosis in a Mouse Model: Reducing Female Ovary Function with an Increase in Age by Proteomics Method[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(12):5475-5486.
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