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Assessment of the Risk of Blastomere Biopsy during Preimplantation Genetic Diagnosis in a Mouse Model: Reducing Female Ovary Function with an Increase in Age by Proteomics Method
Yu, Yang1,2,3; Zhao, Yue1,3; Li, Rong1,3; Li, Li1; Zhao, Hongcui1; Li, Min1; Sha, Jiahao4; Zhou, Qi5; Qiao, Jie1,2,3
关键词preimplantation diagnosis embryo biopsy ovary function female fertility proteomics
刊名JOURNAL OF PROTEOME RESEARCH
2013-12-01
DOI10.1021/pr400366j
12期:12页:5475-5486
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]MONOZYGOTIC TWINS ; PROTEASOME SUBUNIT ; BIRTH-DEFECTS ; EMBRYO BIOPSY ; PROTEIN ; MICE ; EXPRESSION ; FAILURE ; ANDROSTENEDIONE ; LIPODYSTROPHY
英文摘要

Preimplantation genetic diagnosis (PGD) is important for screening genetic and chromosome mutations in embryos so that the efficiency of assisted reproductive treatment can be increased and birth defects can be decreased; however, some studies have reported a risk from this technology as well as other assisted reproductive technologies. We have developed a blastomere biopsy mouse model to assess the potential effects of blastomere biopsy that was one key procedure in PGD on the fertility of female mice at different ages. We showed that female fertility was decreased in the biopsied mouse model with an increase in age. Moreover, the ovarian weight, serum hormone levels, and the number of primordial, primary, preantral, and antral stage follicles were also decreased in the middle-aged biopsied mouse model. To elucidate the underlying molecular mechanism, we did proteomics analysis on ovarian tissues from puberty biopsied and nonbiopsied mice of the 23 differentially expressed proteins that were screened for in both groups, 3 proteins (PSMB8, ALDH1A1, and HSPA4) were selected and identified by Western blotting and quantitative RT-PCR methods, which showed the 3 proteins to regulate 12 cellular pathways. Furthermore, these three proteins were shown to be located in ovarian tissues, and the dynamic changes of expression profiling in middle-aged biopsied and nonbiopsied mice were demonstrated. The present study showed that blastomere biopsy technology impairs fertility when mice are middle-aged, which possibly resulted in abnormal expression profiling of PSMB8, ALDH1A1, and HSPA4 proteins. Thus, additional studies should be performed to assess the overall risk of blastomere biopsies during PGD procedures.

语种英语
WOS记录号WOS:000328231300011
项目编号2011CB944504 ; 2014CB943203 ; 31371521 ; 31000661
资助机构Ministry of Science and Technology of China (973 program) ; National Natural Science Funds for General Program ; National Natural Science Funds for Young Scholar
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60313
专题北京大学第三临床医学院_生殖医学中心
北京大学第二临床医学院_麻醉科
北京大学第三临床医学院_妇产科
作者单位1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
4.Nanjing Med Univ, Dept Histol & Embryol, Lab Reprod Med, Nanjing 210029, Jiangsu, Peoples R China
5.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714
Yu, Yang,Zhao, Yue,Li, Rong,et al. Assessment of the Risk of Blastomere Biopsy during Preimplantation Genetic Diagnosis in a Mouse Model: Reducing Female Ovary Function with an Increase in Age by Proteomics Method[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(12):5475-5486.
APA Yu, Yang.,Zhao, Yue.,Li, Rong.,Li, Li.,Zhao, Hongcui.,...&Qiao, Jie.(2013).Assessment of the Risk of Blastomere Biopsy during Preimplantation Genetic Diagnosis in a Mouse Model: Reducing Female Ovary Function with an Increase in Age by Proteomics Method.JOURNAL OF PROTEOME RESEARCH,12(12),5475-5486.
MLA Yu, Yang,et al."Assessment of the Risk of Blastomere Biopsy during Preimplantation Genetic Diagnosis in a Mouse Model: Reducing Female Ovary Function with an Increase in Age by Proteomics Method".JOURNAL OF PROTEOME RESEARCH 12.12(2013):5475-5486.
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