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学科主题: 临床医学
题名:
Cathepsin D is secreted from M-BE cells: Its potential role as a biomarker of lung cancer
作者: Lou, Xiaomin; Xiao, Ting; Zhao, Kang; Wang, Hao; Zheng, Hongwei; Lin, Dongmei; Lu, Youyong; Gao, Yanning; Cheng, Shujun; Liu, Siqi; Xu, Ningzhi
关键词: lung cancer ; conditioned media ; secretory protein ; cathepsin D
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2007-03-01
DOI: 10.1021/pr060422t
卷: 6, 期:3, 页:1083-1092
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: BREAST-CANCER ; PROCATHEPSIN-D ; D EXPRESSION ; PROTEIN ; CARCINOMA ; SERUM ; MANNOSE-6-PHOSPHATE ; PHOSPHORYLATION ; ADENOCARCINOMA ; FIBROBLASTS
英文摘要:

The early diagnosis of lung cancer is an effective approach to reduce the mortality caused by malignancy. To explore serum biomarkers of lung cancer at early stage, M-BE, a SV40T-transformed human bronchial epithelial cell line with the phenotypic features of early tumorigenesis at high passage, was cultured in the conditioned media to collect its secretory proteins. The proteins secreted from different passage M-BE cells were extracted and then separated by two-dimensional electrophoresis (2-DE). MALDI-TOF/TOF mass spectrometry was adopted to identify the passage-dependent 2-DE spots. Totally, 47 proteins were identified, including 23 that were up-regulated and 24 that were down-regulated. Of these proteins, cathepsin D was a typical secretory protein that exhibited the increased abundance either in culture media or in cells during passaging. Furthermore, the proteomic conclusions were validated in the clinical samples of lung cancer patients. When sandwich ELISA was used, the concentrations of cathepsin D in plasma showed significant differences between lung squamous cell carcinomas (SCC, 104 cases) and normal donors (36 cases, p <= 0.015). When tissue microarray (TMA) was used, cathepsin D expression levels in SCC tissues (178 cases) were significantly higher than those in normal donors (40 cases, p < 0.001). The present study has revealed that M-BE cells at different passages could secrete or release some proteins into the living environment, which might serve as the potential resource for exploring the biomarkers of lung cancer.

语种: 英语
WOS记录号: WOS:000244638400016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60316
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Chinese Acad Sci, Div Proteom, Genom Inst, Beijing 101318, Peoples R China
2.Chinese Acad Med Sci, Dept Etiol & Carcinogenesis, Canc Inst & Hosp, Beijing 100021, Peoples R China
3.Peking Union Med Coll, Beijing 100021, Peoples R China
4.Peking Univ, Sch Oncol, Inst Canc Res, Beijing 100034, Peoples R China
5.Chinese Acad Med Sci, Lab Cell & Mol Biol, Canc Inst & Canc Hosp, Beijing 100021, Peoples R China
6.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Lou, Xiaomin,Xiao, Ting,Zhao, Kang,et al. Cathepsin D is secreted from M-BE cells: Its potential role as a biomarker of lung cancer[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(3):1083-1092.
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