IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways
Ouyang, Jue1,2,3,5; Wu, Minghua1,2,3,5; Huang, Chen4; Cao, Li1,2,3; Li, Guiyuan1,2,3,5
关键词oxidored-nitro domain containing protein 1 proliferation apoptosis mitochondria nasopharyngeal carcinoma cervical cancer
刊名ONCOLOGY REPORTS
2013
DOI10.3892/or.2012.2101
29期:1页:79-86
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]NF-KAPPA-B ; EXPRESSION ; P53 ; GENE ; NOR1 ; HYPERMETHYLATION ; ALLELOTYPE ; ACTIVATION ; ANTIBODY ; DELETION
英文摘要

Oxidored-nitro domain containing protein 1 (NOR1) is a novel member of the nitroreductase family that was first isolated as a tumor suppressor gene from human nasopharyngeal carcinoma (NPC). However, the role of NOR1 gene dysfunction in human cancers has not been addressed. We analyzed the expression of NOR1 in various human cancer and benign tissue specimens and found significant downregulation in nine types of cancer compared with corresponding non-tumor tissues. The recombinant expression vector pCDNA3.1-myc-his-NOR1 was constructed and transfected into human NPC 6-10B nasopharyngeal cancer and HeLa cervical cancer (CCA) cells. We found that stable NOR1 overexpression resulted in suppression of 6-10B and HeLa cell proliferation and led to S phase cell cycle arrest. In addition, NOR1 upregulation enhanced apoptosis in pCDNA3.1-myc-his-NOR1 stably transfected cells, and it also altered the expression of proteins involved in the mitochondria-dependent apoptotic pathway. Furthermore, we also found that the NOR1 protein is a cytoplasmic protein that is partially localized in the mitochondria and endoplasmic reticulum. Therefore, NOR1 is an important tumor suppressor gene associated with NPC and CCA and may play antitumor roles by inhibiting proliferation, preventing colony formation, and promoting the apoptosis of tumor cells via the mitochondrial-dependent apoptotic pathway. However, the precise mechanism behind the NOR1 antitumor effects needs to be investigated further.

语种英语
WOS记录号WOS:000312183900012
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60324
Collection北京大学第三临床医学院
作者单位1.Minist Hlth, Key Lab Carcinogenesis, Changsha, Hunan, Peoples R China
2.Key Lab Nonresolving Inflammat & Canc, Changsha, Hunan, Peoples R China
3.Cent S Univ, Canc Res Inst, Dis Genome Res Ctr, Changsha 410078, Hunan, Peoples R China
4.Minist Educ, Key Lab Carcinogenesis & Canc Invas, Changsha, Hunan, Peoples R China
5.Peking Univ, Hosp 3, Med Res Ctr, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Ouyang, Jue,Wu, Minghua,Huang, Chen,et al. Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways[J]. ONCOLOGY REPORTS,2013,29(1):79-86.
APA Ouyang, Jue,Wu, Minghua,Huang, Chen,Cao, Li,&Li, Guiyuan.(2013).Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways.ONCOLOGY REPORTS,29(1),79-86.
MLA Ouyang, Jue,et al."Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways".ONCOLOGY REPORTS 29.1(2013):79-86.
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