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学科主题: 临床医学
题名:
Down-regulation of CXCR4 expression in MT4 cells by a recombinant vector expressing antisense RNA to CXCR4 and its potential anti-HIV-1 effect
作者: Xing, HC; Xu, XY; Liu, Z; Wang, QH; Yu, M; Si, CW
刊名: JAPANESE JOURNAL OF INFECTIOUS DISEASES
发表日期: 2004-06-01
卷: 57, 期:3, 页:91-96
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Infectious Diseases
研究领域[WOS]: Infectious Diseases
关键词[WOS]: IMMUNODEFICIENCY-VIRUS TYPE-1 ; HIV-1 INFECTION ; T-CELL ; RESISTANCE ; GENE ; INHIBITION ; INDIVIDUALS ; PREVALENCE ; CORECEPTOR ; MACROPHAGE
英文摘要:

CXC-chemokine receptor (CXCR4) is one principle coreceptor for the entry of T cell line (T)tropic HIV-1 virus into a cell. In order to find more efficacious therapeutic possibilities for people with an HIV-1 infection, we explored the inhibitory effects of antisense RNA on CXCR4 expression in MT4 cells. First, we used RT-PCR to obtain DNA fragments from healthy adult peripheral blood mononuclear cells; these fragments targeted the initiation region of CXCR4 mRNA translation. We then constructed a recombinant retroviral vector, pLXSN-X4a (containing antisense RNA to CXCR4). After packaging by PA317 cells, the pseudovirion of the recombinant vector had formed and succeeded in transfecting MT4 cells (a kind of T-tropic HIV-1 susceptibility cell line). The PCR and RT-PCR results showed that the recombinant vector had integrated into the genome of MT4 cells and had been transcribed. The expression of CXCR4 on the surface of MT4 cells transfected with antisense RNA was reduced by 30%, compared with those cells transfected with blank vector or untransfected cells. No change in the DNA synthesis rates or in cell proliferation was found in any of the transfected cells. After a challenge with HIV-1 SF33, the cells transfected with antisense RNA vector (pLXSN-X4a) produced reduced p24 levels compared with the cells transfected with blank vector (pLXSN) or untransfected cells. These results indicated that these CXCR4-antisense expressing cells could resist T-tropic HIV-1 infection and could retain normal biological functions. These studies provide useful data for further experiments in this area.

语种: 英语
WOS记录号: WOS:000222399700001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60352
Appears in Collections:北京大学第一临床医学院_感染疾病科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100034, Peoples R China
2.Zhejiang Univ, First Hosp, Dept Infect Dis, Hangzhou 310003, Peoples R China
3.China CDC Natl Ctr Aids STD Prevent & Control, Beijing 100034, Peoples R China

Recommended Citation:
Xing, HC,Xu, XY,Liu, Z,et al. Down-regulation of CXCR4 expression in MT4 cells by a recombinant vector expressing antisense RNA to CXCR4 and its potential anti-HIV-1 effect[J]. JAPANESE JOURNAL OF INFECTIOUS DISEASES,2004,57(3):91-96.
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