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Cytotoxic effects of sodium dodecyl benzene sulfonate on human keratinocytes are not associated with proinflammatory cytokines expression
Mu Zhanglei; Liu Xiaojing; Zhao Yan; Zhang Jianzhong
关键词sodium dodecyl benzene sulfonate sodium lauryl sulfate keratinocytes cytotoxicity cytokines
刊名CHINESE MEDICAL JOURNAL
2014-11-05
DOI10.3760/cma.j.issn.0366-6999.20140689
127期:21页:3777-3781
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]IRRITANT CONTACT-DERMATITIS ; LIPID-METABOLIZING ENZYMES ; MESSENGER-RNA EXPRESSION ; LAURYL SULFATE ; CELL-LINE ; SKIN ; INFLAMMATION ; SURFACTANTS ; DIFFERENTIATION ; DETERGENTS
英文摘要

Background Keratinocytes play a crucial role in the biological function of skin barrier. The relationship between sodium lauryl sulfate (SLS) and keratinocytes has been studied. However, the cytotoxicity and effects of sodium dodecyl benzene sulfonate (SOBS), a common detergent similar to SLS, on keratinocytes are still not known. This study aimed to investigate the effects of SDBS on cytotoxicity and expression of proinflammatory cytokines in cultured human keratinocytes.

Methods This study was carried out using the keratinocytes cell line, HaCaT cells. The cytotoxicity of SDBS on HaCaT cells was evaluated with cell counting kit-8 (CCK-8) and phase-contrast microscopy. After exposure to different concentrations of SDBS, the total RNA of the HaCaT cells was extracted for evaluating the relative mRNA expression of IL-1 alpha, IL-6, IL-8, and TNF-alpha by qPCR. The supernatants of cells were collected for measuring the levels of IL-6 and IL-8 by enzyme-linked immunosorbent assay (ELISA).

Results SDBS at concentrations of 20 mu g/ml and over showed direct cytotoxicity and induced morphological changes of the HaCaT cells. The mRNA expressions of IL-1 alpha, IL-6, IL-8, and TNF-alpha in different concentrations of SOBS at different time were comparable with that of controls. SDBS at concentrations of 5, 10, and 15 pg/ml had no significant effects on IL-6 and IL-8 excretion from HaCaT cells after,24-hour exposure. Moreover, no significant effects on the IL-6 and IL-8 excretion were found after 10 and 15 mu g/ml SDBS stimulations for 6, 12, and 24 hours, respectively.

Conclusion SDBS at higher concentrations had cytotoxicity on HaCaT cells but had no effects on the mRNA expression of IL-1 alpha, IL-6, IL-8, and TNF-alpha, that was different from SLS.

语种英语
WOS记录号WOS:000346329300018
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60353
专题北京大学第二临床医学院_皮科
北京大学第三临床医学院_麻醉科
作者单位Peking Univ, Peoples Hosp, Dept Dermatol, Beijing 100044, Peoples R China
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GB/T 7714
Mu Zhanglei,Liu Xiaojing,Zhao Yan,et al. Cytotoxic effects of sodium dodecyl benzene sulfonate on human keratinocytes are not associated with proinflammatory cytokines expression[J]. CHINESE MEDICAL JOURNAL,2014,127(21):3777-3781.
APA Mu Zhanglei,Liu Xiaojing,Zhao Yan,&Zhang Jianzhong.(2014).Cytotoxic effects of sodium dodecyl benzene sulfonate on human keratinocytes are not associated with proinflammatory cytokines expression.CHINESE MEDICAL JOURNAL,127(21),3777-3781.
MLA Mu Zhanglei,et al."Cytotoxic effects of sodium dodecyl benzene sulfonate on human keratinocytes are not associated with proinflammatory cytokines expression".CHINESE MEDICAL JOURNAL 127.21(2014):3777-3781.
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