IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所
学科主题临床医学
Determination of Erythromycylamine in Human Serum by High Performance Liquid Chromatography with Pre-column Derivatization
Wei Min-Ji; Zhao Cai-Yun; Lue Yuan; Zhang Pu; Zhang Ming; Liu Yan
关键词Dirithromycin erythromycylamine high performance liquid chromatography 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole precolumn derivatization
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2009-06-01
37期:6页:907-910
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]DIRITHROMYCIN ; PHARMACOKINETICS
英文摘要

A high performance liquid chromatographic method using fluorescence detection is described for the analysis of erythromycyclamine, an active metabolite of dirithromycin in human blood. Erythromycyclamine is extracted from serum by methyl-tert-butyl ether and involves pre-(column derivatization with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) and reverse-phase chromatography on C(18) column. The mobile phase was 0.01 mol/L potassium phosphate buffer (pH 6.0) and acetonitrile (80:20) at a flow rate of 1.0 mL/min. desloratadine was used as internal standard. The standard curve was linear in the range of 0.010 - 1.245 mg/L of erythromycyclamine in human serum. The intra-day and inter-day precision studies showed good reproducibility with relative standard deviation less than 9% for all the analytes. The limit of quantification was 10 mu g/L. This method has been applied to a pharmacokinetic study after administration of 750 mg dirithromycin in 18 normal subjects.

语种中文
WOS记录号WOS:000267385100025
Citation statistics
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60361
Collection北京大学第一临床医学院_临床药理研究所
作者单位Peking Univ, Inst Clin Pharmacol, Hosp 1, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Wei Min-Ji,Zhao Cai-Yun,Lue Yuan,et al. Determination of Erythromycylamine in Human Serum by High Performance Liquid Chromatography with Pre-column Derivatization[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(6):907-910.
APA Wei Min-Ji,Zhao Cai-Yun,Lue Yuan,Zhang Pu,Zhang Ming,&Liu Yan.(2009).Determination of Erythromycylamine in Human Serum by High Performance Liquid Chromatography with Pre-column Derivatization.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(6),907-910.
MLA Wei Min-Ji,et al."Determination of Erythromycylamine in Human Serum by High Performance Liquid Chromatography with Pre-column Derivatization".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.6(2009):907-910.
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