北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 消化内科  > 期刊论文
学科主题: 临床医学
题名:
Effect of cyclo-oxygenase inhibitors on Helicobacter pylori susceptibility to metronidazole and clarithromycin
作者: Gu, Q; Xia, HHX; Wang, WH; Wang, JD; Wong, WM; Chan, AOO; Yuen, MF; Lam, SK; Cheung, HKL; Liu, XG; Wong, BCY
刊名: ALIMENTARY PHARMACOLOGY & THERAPEUTICS
发表日期: 2004-09-15
DOI: 10.1111/j.1365-2036.2004.02168.x
卷: 20, 期:6, 页:675-681
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Pharmacology & Pharmacy
研究领域[WOS]: Gastroenterology & Hepatology ; Pharmacology & Pharmacy
关键词[WOS]: GASTRIC EPITHELIAL-CELLS ; NEGATIVE DUODENAL-ULCER ; MONGOLIAN GERBILS ; ANTIBIOTIC-RESISTANCE ; MUCOSAL INJURY ; KAPPA-B ; APOPTOSIS ; CANCER ; EXPRESSION ; INDUCTION
英文摘要:

Background: We previously reported that aspirin inhibited Helicobacter pylori growth and suppressed the mutagenic effect of metronidazole.

Aim: To determine the effects of a cyclo-oxygenase (COX)-2-specific inhibitor, SC-236, and a non-selective COX inhibitor, indometacin, on the growth, urease activity and antimicrobial susceptibility of H. pylori.

Methods: Three H. pylori reference strains, and 18 clinical isolates were treated with SC-236 or indometacin for 24 and 48 h. Growth, urease activity and susceptibility to clarithromycin and metronidazole of the bacteria were assessed by viable colony counting, spectrophotometry and E-test respectively.

Results: SC-236 and indometacin inhibited H. pylori growth in a dose-dependent manner with the lowest inhibitory concentrations of 0.03 and 0.1 mM, and the lethal concentrations of 0.09 and 0.3 mM, respectively. The numbers of CFU/mL in Brucella broth containing 0.09 mM SC-236 were 2 log lower at 24 h, and even 3 log lower at 48 h than that at 0 h (P = 0.035, compared with the vehicle control). Treatment of 0.3 mM indometacin reduced the number of CFU/mL by 1 log at 24 h compared with that at 0 h (P = 0.037 compared with the vehicle control). Helicobacter pylori urease activity began to decrease with 0.06 mM SC-236 at 24 h (P = 0.016), and 0.3 mM indometacin at 48 h (P = 0.025). MICs of metronidazole and clarithromycin against H. pylori were decreased significantly in the presence of 0.03 mM SC-236 or 0.1 mM indometacin (all P < 0.001).

Conclusion: Both SC-236 and indometacin suppressed the growth and urease activity of H. pylori in a dose-dependent manner, and increased its susceptibility to the antibiotics.

语种: 英语
WOS记录号: WOS:000223713300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60363
Appears in Collections:北京大学第一临床医学院_消化内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ, Hosp 1, Dept Gastroenterol, Beijing 100871, Peoples R China

Recommended Citation:
Gu, Q,Xia, HHX,Wang, WH,et al. Effect of cyclo-oxygenase inhibitors on Helicobacter pylori susceptibility to metronidazole and clarithromycin[J]. ALIMENTARY PHARMACOLOGY &amp; THERAPEUTICS,2004,20(6):675-681.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gu, Q]'s Articles
[Xia, HHX]'s Articles
[Wang, WH]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gu, Q]‘s Articles
[Xia, HHX]‘s Articles
[Wang, WH]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace