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学科主题: 临床医学
题名:
Conversion of Adipose-Derived Stem Cells into Natural Killer-Like Cells with Anti-Tumor Activities in Nude Mice
作者: Ning, Hongxiu1; Lei, Hong-En2; Xu, Yong-De2; Guan, Rui-Li2; Venstrom, Jeffrey M.3; Lin, Guiting1; Lue, Tom F.1; Xin, Zhongcheng2; Lin, Ching-Shwun1
刊名: PLOS ONE
发表日期: 2014-08-27
DOI: 10.1371/journal.pone.0106246
卷: 9, 期:8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: BLOOD CD34(+) CELLS ; HEMATOPOIETIC STEM ; STROMAL CELLS ; NK CELLS ; TISSUE ; IDENTIFICATION ; IMMUNOTHERAPY ; GENERATION ; THERAPY ; INNATE
英文摘要:

Efforts to develop peripheral blood-derived nature killer (NK) cells into therapeutic products have been hampered by these cells′ low abundance and histoincompatibility. On the other hand, derivation of NK-like cells from more abundant cell sources such as embryonic stem cells (ESCs) and umbilical cord blood (UCB) requires the selection of rare CD34+ cells. Thus, we sought to convert adipose-derived stem cells (ADSCs), which are abundant and natively CD34+, into NK-like cells. When grown in hematopoietic induction medium, ADSCs formed sphere clusters and expressed hematopoietic markers CD34, CD45, and KDR. Further induction in NK cell-specific medium resulted in a population of cells that expressed NK cell marker CD56, and thus termed ADSC-NK. Alternatively, the hematopoietically induced ADSCs were transduced with NK cell-specific transcription factor E4BP4 prior to induction in NK cell-specific medium. This latter population of cells, termed ADSC-NKE, expressed CD56 and additional NK cell markers such as CD16, CD94, CD158, CD314, FasL, and NKp46. ADSC-NKE was as potent as NK leukemia cell NKL in killing breast cancer cell MCF7 and prostate cancer cells DU145, PC3, LnCap, DuPro, C4-2 and CWR22, but exhibited no killing activity toward normal endothelial and smooth muscle cells. In nude mice test ADSC-NKE was able to significantly delay the progression of tumors formed by MCF7 and PC3. When injected into immunocompetent rats, ADSC-NKE was detectable in bone marrow and spleen for at least 5 weeks. Together, these results suggest that ADSCs can be converted into NK-like cells with anti-tumor activities.

语种: 英语
所属项目编号: DK64538 ; DK045370 ; DK069655 ; 81272531
项目资助者: National Institutes of Health ; National Natural Science Fund of China
WOS记录号: WOS:000340880900098
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60366
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
2.Peking Univ, Hosp 1, Androl Ctr, Beijing 100871, Peoples R China
3.Univ Calif San Francisco, Sch Med, Dept Med, San Francisco, CA USA

Recommended Citation:
Ning, Hongxiu,Lei, Hong-En,Xu, Yong-De,et al. Conversion of Adipose-Derived Stem Cells into Natural Killer-Like Cells with Anti-Tumor Activities in Nude Mice[J]. PLOS ONE,2014,9(8).
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