IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Analysis of Translocation of the CagA Protein and Induction of a Scattering Phenotype in AGS Cells Infected with Helicobacter pylori
Liang, Xian-Hong2; Zhang, Ya-Nan1,3; Wang, Yong-Jun2; Kang, Xi-Xiong3
关键词CagA Western CagA-specific sequence (WSS) Eastern CagA-specific sequence (ESS) Hummingbird phenotype
刊名BIOMEDICAL AND ENVIRONMENTAL SCIENCES
2009-10-01
22期:5页:394-400
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Environmental Sciences ; Public, Environmental & Occupational Health
研究领域[WOS]Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
关键词[WOS]GASTRIC EPITHELIAL-CELLS ; DIVISION-RELATED GENE ; PATHOGENICITY ISLAND ; IV SECRETION ; TYROSINE PHOSPHORYLATION ; BIOLOGICAL-ACTIVITY ; VIRULENCE FACTORS ; PHOSPHATASE ; BACTERIAL ; REVEALS
英文摘要

Objective To investigate whether the presence of structured CagA proteins in Western- and Eastern-type Helicobacter pylori (H. pylori) induces different incidences of gastric diseases. Methods CagA and phosphorylated CagA. were expressed in AGS gastric epithelial cells infected with wild type and mutant strains. The ability of individual CagA was determined by immunoprecipitation and Western blot assay. Morphological changes of these cells were observed under microscope to evaluate the appearance of elongation hummingbird phenotype. Results The sizes of CagA proteins indifferent strains were different, and no phosphorylated CagA proteins were detected in wild-type strains. Meanwhile, the kinetics of CagA status in AGS infected with H. pylori was detected. The molecular weight of phosphorylated CagA with the same size of CagA proteins in H. pylori was different in infections with different wild-type strains. CagA and phosphorylated CagA increased in a time-dependent manner after the infection. The hummingbird phenotype with H. pylori for time-course was observed under microscope. Instead of HPK5 strain, the wild-type 26695 strain induced hummingbird phenotype in a time-dependent manner. Conclusion Translocation and phosphorylation of CagA are necessary, but not sufficient, for the induction of hummingbird phenotype in AGS cells.

语种英语
WOS记录号WOS:000271914300006
项目编号20070420277
资助机构China Postdoctoral Science Foundation
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60373
专题北京大学基础医学院_病原生物学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
2.Capital Med Univ, Beijing Tiantan Hosp, Dept Neurol, Beijing 100050, Peoples R China
3.Capital Med Univ, Beijing Tiantan Hosp, Dept Lab, Beijing 100050, Peoples R China
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GB/T 7714
Liang, Xian-Hong,Zhang, Ya-Nan,Wang, Yong-Jun,et al. Analysis of Translocation of the CagA Protein and Induction of a Scattering Phenotype in AGS Cells Infected with Helicobacter pylori[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2009,22(5):394-400.
APA Liang, Xian-Hong,Zhang, Ya-Nan,Wang, Yong-Jun,&Kang, Xi-Xiong.(2009).Analysis of Translocation of the CagA Protein and Induction of a Scattering Phenotype in AGS Cells Infected with Helicobacter pylori.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,22(5),394-400.
MLA Liang, Xian-Hong,et al."Analysis of Translocation of the CagA Protein and Induction of a Scattering Phenotype in AGS Cells Infected with Helicobacter pylori".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 22.5(2009):394-400.
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