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学科主题: 基础医学
题名:
Analysis of Translocation of the CagA Protein and Induction of a Scattering Phenotype in AGS Cells Infected with Helicobacter pylori
作者: Liang, Xian-Hong2; Zhang, Ya-Nan1,3; Wang, Yong-Jun2; Kang, Xi-Xiong3
关键词: CagA ; Western CagA-specific sequence (WSS) ; Eastern CagA-specific sequence (ESS) ; Hummingbird phenotype
刊名: BIOMEDICAL AND ENVIRONMENTAL SCIENCES
发表日期: 2009-10-01
卷: 22, 期:5, 页:394-400
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Environmental Sciences ; Public, Environmental & Occupational Health
研究领域[WOS]: Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
关键词[WOS]: GASTRIC EPITHELIAL-CELLS ; DIVISION-RELATED GENE ; PATHOGENICITY ISLAND ; IV SECRETION ; TYROSINE PHOSPHORYLATION ; BIOLOGICAL-ACTIVITY ; VIRULENCE FACTORS ; PHOSPHATASE ; BACTERIAL ; REVEALS
英文摘要:

Objective To investigate whether the presence of structured CagA proteins in Western- and Eastern-type Helicobacter pylori (H. pylori) induces different incidences of gastric diseases. Methods CagA and phosphorylated CagA. were expressed in AGS gastric epithelial cells infected with wild type and mutant strains. The ability of individual CagA was determined by immunoprecipitation and Western blot assay. Morphological changes of these cells were observed under microscope to evaluate the appearance of elongation hummingbird phenotype. Results The sizes of CagA proteins indifferent strains were different, and no phosphorylated CagA proteins were detected in wild-type strains. Meanwhile, the kinetics of CagA status in AGS infected with H. pylori was detected. The molecular weight of phosphorylated CagA with the same size of CagA proteins in H. pylori was different in infections with different wild-type strains. CagA and phosphorylated CagA increased in a time-dependent manner after the infection. The hummingbird phenotype with H. pylori for time-course was observed under microscope. Instead of HPK5 strain, the wild-type 26695 strain induced hummingbird phenotype in a time-dependent manner. Conclusion Translocation and phosphorylation of CagA are necessary, but not sufficient, for the induction of hummingbird phenotype in AGS cells.

语种: 英语
所属项目编号: 20070420277
项目资助者: China Postdoctoral Science Foundation
WOS记录号: WOS:000271914300006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60373
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
2.Capital Med Univ, Beijing Tiantan Hosp, Dept Neurol, Beijing 100050, Peoples R China
3.Capital Med Univ, Beijing Tiantan Hosp, Dept Lab, Beijing 100050, Peoples R China

Recommended Citation:
Liang, Xian-Hong,Zhang, Ya-Nan,Wang, Yong-Jun,et al. Analysis of Translocation of the CagA Protein and Induction of a Scattering Phenotype in AGS Cells Infected with Helicobacter pylori[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2009,22(5):394-400.
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