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Maturation outcomes are improved following Cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing
Combelles, Catherine M. H.1; Ceyhan, S. Temel2; Wang, Haiyan2,3; Racowsky, Catherine2
关键词Immature oocyte Choline-based slow-freezing Cryoleaf vitrification Spindle Chromosomes
刊名JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
2011-12-01
DOI10.1007/s10815-011-9674-x
28期:12页:1183-1192
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]IN-VITRO MATURATION ; GERMINAL VESICLE STAGE ; MATURED HUMAN OOCYTES ; METAPHASE-II SPINDLE ; BOVINE OOCYTES ; DEVELOPMENTAL COMPETENCE ; COOLING CRYOPRESERVATION ; CYTOPLASMIC MATURATION ; EMBRYO DEVELOPMENT ; MAMMALIAN OOCYTES
英文摘要

Purpose The cryopreservation of immature oocytes permits oocyte banking for patients at risk of losing their fertility. However, the optimum protocol for such fertility preservation remains uncertain.

Methods The present study investigated the survival, maturation, cytoskeletal and chromosome organization of sibling immature oocytes leftover from controlled ovarian stimulation cycles, that were either slow-frozen (with choline-substitution) or vitrified. A comparison group included oocytes that were never cryopreserved.

Results Among the three groups, comparable rates were observed for both survival (67-70%) and polar body extrusion (59-79%). Significantly more oocytes underwent spontaneous activation after IVM following slow-freezing compared with either vitrification or no cryopreservation. Likewise, the incidence of spindle abnormalities was greatest in the slow-frozen group, with no differences in spindle morphometrics or chromosome organization.

Conclusions While the overall incidence of mature oocytes with normal bipolar spindles from warmed immature oocytes was low, the yield using Cryoleaf vitrification was slightly superior to choline-based slow-freezing.

语种英语
WOS记录号WOS:000298228400009
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60379
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Middlebury Coll, Dept Biol, Middlebury, VT 05753 USA
2.Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Obstet Gynecol & Reprod Biol, Boston, MA 02115 USA
3.Peking Univ, Hosp 3, Ctr Reprod Med, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
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GB/T 7714
Combelles, Catherine M. H.,Ceyhan, S. Temel,Wang, Haiyan,et al. Maturation outcomes are improved following Cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing[J]. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,2011,28(12):1183-1192.
APA Combelles, Catherine M. H.,Ceyhan, S. Temel,Wang, Haiyan,&Racowsky, Catherine.(2011).Maturation outcomes are improved following Cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing.JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,28(12),1183-1192.
MLA Combelles, Catherine M. H.,et al."Maturation outcomes are improved following Cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing".JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 28.12(2011):1183-1192.
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