IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Structure elucidation and immunological function analysis of a novel beta-glucan from the fruit bodies of Polyporus umbellatus (Pers.) Fries
Dai, Hui2; Han, Xiao-Qiang3; Gong, Fang-Yuan1,4; Dong, Hongliang1,4; Tu, Peng-Fei3; Gao, Xiao-Ming1,4
关键词beta-glucan immunostimulation lymphocyte macrophage structure
刊名GLYCOBIOLOGY
2012-12-01
DOI10.1093/glycob/cws099
22期:12页:1673-1683
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
资助者PCSIRT ; National Foundation of Natural Science of China ; National Key Basic Research Programs ; Research Fund for the Doctoral Program of Higher Education ; Priority Academic Program Development of Jiangsu Province Higher Education Institutions ; PCSIRT ; National Foundation of Natural Science of China ; National Key Basic Research Programs ; Research Fund for the Doctoral Program of Higher Education ; Priority Academic Program Development of Jiangsu Province Higher Education Institutions
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]SYSTEMIC-LUPUS-ERYTHEMATOSUS ; SERUM ANTIBODIES ; ALDITOL ACETATES ; ELEVATED LEVELS ; DECTIN-1 ; INDUCTION ; RECEPTOR ; CELLS ; POLYSACCHARIDES ; ZYMOSAN
英文摘要

beta-Glucans derived from various sources such as yeast cell walls and medicinal mushrooms are considered as valuable biological response modifiers for their ability to enhance the activity of immune cells, aid in wound healing and help prevent infections. We herein characterize the structure of a novel water-soluble polysaccharide (Zhuling polysaccharide, ZPS) from the fruit bodies of medicinal mushroom Polyporus umbellatus and investigate its immunobiological function. ZPS has a molecular mass of 2.27 x 10(3) kDa and contains > 90% d-glucose as its monosaccharide constituent. On the basis of partial acid hydrolysis, methylation analysis, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy and the ideal repeating unit of ZPS is established: (1 -> 6, 1 -> 4)-linked beta-d-glucopyranosyl backbone, substituted at O-3 position of (1 -> 6)-linked beta-d-glucopyranosyl by (1 -> 3)-linked beta-d-glucopyranosyl branches. ZPS consists of approximately 2930 repeating units, each contains a side chain of no more than three residues in length. Functionally, ZPS is a potent activator of B cells, macrophages and dendritic cells. Depletion of ZPS branches causes a substantial reduction in its ability not only to activate B cells in vitro but also to elicit specific IgM production in vivo. Virtually all healthy human subjects possess high-titer circulating antibodies against ZPS backbone, suggesting that ZPS epitope is shared by environmental antigens capable of eliciting adaptive humoral responses in the population.

语种英语
所属项目编号IRT1075 ; 30890142/31070781/81102253 ; 2010CB529102 ; 20100001120042
资助者PCSIRT ; National Foundation of Natural Science of China ; National Key Basic Research Programs ; Research Fund for the Doctoral Program of Higher Education ; Priority Academic Program Development of Jiangsu Province Higher Education Institutions ; PCSIRT ; National Foundation of Natural Science of China ; National Key Basic Research Programs ; Research Fund for the Doctoral Program of Higher Education ; Priority Academic Program Development of Jiangsu Province Higher Education Institutions
WOS记录号WOS:000310630900004
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60393
Collection北京大学基础医学院_免疫学系
作者单位1.Soochow Univ, Inst Biol & Med Sci, Suzhou 215123, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Sch Basic Med Sci, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
4.Key Lab Infect & Immun Jiangsu Prov, Suzhou, Peoples R China
Recommended Citation
GB/T 7714
Dai, Hui,Han, Xiao-Qiang,Gong, Fang-Yuan,et al. Structure elucidation and immunological function analysis of a novel beta-glucan from the fruit bodies of Polyporus umbellatus (Pers.) Fries[J]. GLYCOBIOLOGY,2012,22(12):1673-1683.
APA Dai, Hui,Han, Xiao-Qiang,Gong, Fang-Yuan,Dong, Hongliang,Tu, Peng-Fei,&Gao, Xiao-Ming.(2012).Structure elucidation and immunological function analysis of a novel beta-glucan from the fruit bodies of Polyporus umbellatus (Pers.) Fries.GLYCOBIOLOGY,22(12),1673-1683.
MLA Dai, Hui,et al."Structure elucidation and immunological function analysis of a novel beta-glucan from the fruit bodies of Polyporus umbellatus (Pers.) Fries".GLYCOBIOLOGY 22.12(2012):1673-1683.
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