北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Regio- and stereo-selective biotransformation of 2 alpha,5 alpha,10 beta,14 beta-tetra-acetoxy-4(20), 11-taxadiene by Ginkgo cell suspension cultures
作者: Dai, JG; Ye, M; Guo, HZ; Zhu, WH; Zhang, DO; Hu, Q; Zheng, JH; Guo, D
关键词: taxane ; sinenxan A ; biotransformation ; enzymes ; cell suspension cultures ; Ginkgo biloba
刊名: TETRAHEDRON
发表日期: 2002-07-08
卷: 58, 期:28, 页:5659-5668
收录类别: IC ; SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Organic
研究领域[WOS]: Chemistry
关键词[WOS]: BIOLOGICAL EVALUATION ; MULTIDRUG-RESISTANCE ; TAXOL BIOSYNTHESIS ; PACIFIC YEW ; AGENT ; CYTOCHROME-P450 ; MECHANISM ; SYNTHASE ; ANALOGS ; TAXUS
英文摘要:

Ginkgo biloba cell suspension cultures were used to bioconvert sinenxan A, 2alpha,5alpha, 10beta, 14beta-tetra-acetoxy-4(20), 11-taxadiene, a taxoid isolated from callus tissue cultures of Taxus spp. Besides two major products, 9alpha-hydroxy-2alpha,5alpha,10beta,14beta-tetra-acetoxy-4(20), 11-taxadiene 1 and 9alpha,10beta-dihydroxy-2alpha,5alpha,14beta-triacetoxy-4(20), 11-taxadiene 2, additional six minor products were obtained and five of them identified as new compounds. On the basis of chemical and spectral data, their structures were identified as 9alpha,14beta-dihydroxy-2alpha,5alpha,10beta-triacetoxy-4(20), 11-taxadiene 3, 6alpha,10beta-dihydroxy-2alpha,5alpha,14beta-triacetoxy-4(20), 11-taxadiene 4, 6alpha,9alpha,10beta-trihydroxy-2alpha,5alpha,14beta-triacetoxy-4(20), 11-taxadiene 5, 9alpha,10beta-O-(propane-2,2-diyl)-2alpha,5alpha,14beta-triacetoxy-4(20), 11-taxadiene 6, 9alpha-hydroxy-2alpha,5alpha,10beta,14beta-tetra-acetoxy-4(20), 11-taxadiene formate 7, 10beta-hydroxy-2alpha,5alpha,9alpha,14beta-tetra-acetoxy-4(20), 11-taxadiene formate 8, respectively. Investigation of the properties of the enzymes responsible for the biocatalysis process of sinenxan A to I and 2 revealed that the enzymes were extracellular and constitutive. Using sinenxan A and the two major products (1 and 2) as indicators, the stage and concentration of sinenxan A added and the kinetics of the biotransformation reaction were investigated. The results showed that: (1) the optimal stage for sinenxan A addition was the logarithmic phase of the cell growth period, in which sinenxan A was almost completely bioconverted, and the biotransformation rates were up to 60 and 20% for 1 and 2, respectively; (2) the optimal concentration of sinenxan A added was 60 mg/L; (3) the substrate was mainly converted into 1 and 2 in the first 48 h after addition and then into the minor products. (C) 2002 Elsevier Science Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000176697100011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60411
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China
3.Peking Union Med Coll, Beijing 100050, Peoples R China

Recommended Citation:
Dai, JG,Ye, M,Guo, HZ,et al. Regio- and stereo-selective biotransformation of 2 alpha,5 alpha,10 beta,14 beta-tetra-acetoxy-4(20), 11-taxadiene by Ginkgo cell suspension cultures[J]. TETRAHEDRON,2002,58(28):5659-5668.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Dai, JG]'s Articles
[Ye, M]'s Articles
[Guo, HZ]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Dai, JG]‘s Articles
[Ye, M]‘s Articles
[Guo, HZ]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace