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学科主题基础医学
Silencing of Long Noncoding RNA AK139328 Attenuates Ischemia/Reperfusion Injury in Mouse Livers
Chen, Zhenzhen1,4; Jia, Shi1,4; Li, Danhua1,4; Cai, Junyan1,4; Tu, Jian1,4; Geng, Bin1,4; Guan, Youfei1,4; Cui, Qinghua1,2,3,4; Yang, Jichun1,4
刊名PLOS ONE
2013-11-27
DOI10.1371/journal.pone.0080817
8期:11
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ISCHEMIA-REPERFUSION INJURY ; ACTIVATED-RECEPTOR-GAMMA ; MOLECULAR-MECHANISMS ; EXPRESSION ; MICE ; PROTECTS ; PATHWAY ; CELLS ; AKT ; HEPATOCYTES
英文摘要

Recently, increasing evidences had suggested that long noncoding RNAs (LncRNAs) are involved in a wide range of physiological and pathophysiological processes. Here we determined the LncRNA expression profile using microarray technology in mouse livers after ischemia/reperfusion treatment. Seventy one LncRNAs were upregulated, and 27 LncRNAs were downregulated in ischemia/reperfusion-treated mouse livers. Eleven of the most significantly deregulated LncRNAs were further validated by quantitative PCR assays. Among the upregulated LncRNAs confirmed by quantitative PCR assays, AK139328 exhibited the highest expression level in normal mouse livers. siRNA-mediated knockdown of hepatic AK139328 decreased plasma aminotransferase activities, and reduced necrosis area in the livers with a decrease in caspase-3 activation after ischemia/reperfusion treatment. In ischemia/reperfusion liver, knockdown of AK139328 increased survival signaling proteins including phosphorylated Akt (pAkt), glycogen synthase kinase 3 (pGSK3) and endothelial nitric oxide synthase (peNOS). Furthermore, knockdown of AK139328 also reduced macrophage infitration and inhibited NF-kappa B activity and inflammatory cytokines expression. In conclusion, these findings revealed that deregulated LncRNAs are involved in liver ischemia/reperfusion injury. Silencing of AK139328 ameliorated ischemia/reperfusion injury in the liver with the activation of Akt signaling pathway and inhibition of NF-kappa B activity. LncRNA AK139328 might be a novel target for diagnosis and treatment of liver surgery or transplantation.

语种英语
WOS记录号WOS:000327652100054
项目编号2012CB517504 ; 2012CB517806 ; 81170791 ; 7122107
资助机构Ministry of Science and Technology ; Natural Science Foundation of China ; "New Century Excellent Talents in Universities" Program from the Ministry of Education of China ; Beijing Natural Science Foundation
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60418
专题北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Beijing 100871, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biomed Informat, Beijing 100871, Peoples R China
3.Peking Univ, Inst Syst Biomed, Beijing 100871, Peoples R China
4.Peking Univ, MOE Key Lab Mol Cardiovasc Sci, Beijing 100871, Peoples R China
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Chen, Zhenzhen,Jia, Shi,Li, Danhua,et al. Silencing of Long Noncoding RNA AK139328 Attenuates Ischemia/Reperfusion Injury in Mouse Livers[J]. PLOS ONE,2013,8(11).
APA Chen, Zhenzhen.,Jia, Shi.,Li, Danhua.,Cai, Junyan.,Tu, Jian.,...&Yang, Jichun.(2013).Silencing of Long Noncoding RNA AK139328 Attenuates Ischemia/Reperfusion Injury in Mouse Livers.PLOS ONE,8(11).
MLA Chen, Zhenzhen,et al."Silencing of Long Noncoding RNA AK139328 Attenuates Ischemia/Reperfusion Injury in Mouse Livers".PLOS ONE 8.11(2013).
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