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学科主题: 基础医学
题名:
Silencing of Long Noncoding RNA AK139328 Attenuates Ischemia/Reperfusion Injury in Mouse Livers
作者: Chen, Zhenzhen1,4; Jia, Shi1,4; Li, Danhua1,4; Cai, Junyan1,4; Tu, Jian1,4; Geng, Bin1,4; Guan, Youfei1,4; Cui, Qinghua1,2,3,4; Yang, Jichun1,4
刊名: PLOS ONE
发表日期: 2013-11-27
DOI: 10.1371/journal.pone.0080817
卷: 8, 期:11
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: ISCHEMIA-REPERFUSION INJURY ; ACTIVATED-RECEPTOR-GAMMA ; MOLECULAR-MECHANISMS ; EXPRESSION ; MICE ; PROTECTS ; PATHWAY ; CELLS ; AKT ; HEPATOCYTES
英文摘要:

Recently, increasing evidences had suggested that long noncoding RNAs (LncRNAs) are involved in a wide range of physiological and pathophysiological processes. Here we determined the LncRNA expression profile using microarray technology in mouse livers after ischemia/reperfusion treatment. Seventy one LncRNAs were upregulated, and 27 LncRNAs were downregulated in ischemia/reperfusion-treated mouse livers. Eleven of the most significantly deregulated LncRNAs were further validated by quantitative PCR assays. Among the upregulated LncRNAs confirmed by quantitative PCR assays, AK139328 exhibited the highest expression level in normal mouse livers. siRNA-mediated knockdown of hepatic AK139328 decreased plasma aminotransferase activities, and reduced necrosis area in the livers with a decrease in caspase-3 activation after ischemia/reperfusion treatment. In ischemia/reperfusion liver, knockdown of AK139328 increased survival signaling proteins including phosphorylated Akt (pAkt), glycogen synthase kinase 3 (pGSK3) and endothelial nitric oxide synthase (peNOS). Furthermore, knockdown of AK139328 also reduced macrophage infitration and inhibited NF-kappa B activity and inflammatory cytokines expression. In conclusion, these findings revealed that deregulated LncRNAs are involved in liver ischemia/reperfusion injury. Silencing of AK139328 ameliorated ischemia/reperfusion injury in the liver with the activation of Akt signaling pathway and inhibition of NF-kappa B activity. LncRNA AK139328 might be a novel target for diagnosis and treatment of liver surgery or transplantation.

语种: 英语
所属项目编号: 2012CB517504 ; 2012CB517806 ; 81170791 ; 7122107
项目资助者: Ministry of Science and Technology ; Natural Science Foundation of China ; "New Century Excellent Talents in Universities" Program from the Ministry of Education of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000327652100054
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60418
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Beijing 100871, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biomed Informat, Beijing 100871, Peoples R China
3.Peking Univ, Inst Syst Biomed, Beijing 100871, Peoples R China
4.Peking Univ, MOE Key Lab Mol Cardiovasc Sci, Beijing 100871, Peoples R China

Recommended Citation:
Chen, Zhenzhen,Jia, Shi,Li, Danhua,et al. Silencing of Long Noncoding RNA AK139328 Attenuates Ischemia/Reperfusion Injury in Mouse Livers[J]. PLOS ONE,2013,8(11).
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