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Effect of hydroxyurea and etoposide on transduction of human bone marrow mesenchymal stem and progenitor cell by adeno-associated virus vectors
Ju, XD; Lou, SQ; Wang, WG; Peng, JQ; Tian, H
关键词simian adenoviruses hydroxyurea etoposide mesoderm stem cells gene transfer techniques
刊名ACTA PHARMACOLOGICA SINICA
2004-02-01
25期:2页:196-202
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]MICE ; DIFFERENTIATION ; REPLICATION ; INTEGRATION ; PARVOVIRUS ; CORECEPTOR ; INFECTION ; SITE ; LUNG
英文摘要

AIM: To study the effect of hydroxyurea and etoposide on transduction of human marrow mesenchymal and progenitor stem cells by adeno-associated-virus (AAV). METHODS: Isolated human bone marrow mesenchymal stem and progenitor cells (hMSCs) were cultured in DMEM containing 10% FBS or 5% FBS and dexamethasone 1 mumol/L respectively. After being treated with hydroxyurea and etoposide, hMSCs were transduced by AAV-LUC. After two days luciferase activity (relative light unites per second or RLU/s) were tested, which indirectly reflected the relative transduction efficiency of different groups, and virus DNA was isolated by Hirt extraction for Southern hybridization. RESULTS: Transduction luciferase activity and transduction efficiency in cultures treated with hydroxyurea and etoposide were significantly higher than that in control cultures. Dividing cells had about 20-fold higher transduction efficiency compared with control cells. Transduction efficiency in stationary cells was about 50 times higher than that in control cells. Southern analysis showed that hydroxyurea and etoposide enhanced second-strand DNA synthesis by rAAV CONCLUSION: Hydroxyurea and etoposide could increase transduction efficiency of hMSCs by AAV vectors, and stationary cells were more sensitive to these drugs than dividing cells.

语种中文
WOS记录号WOS:000188877300012
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60422
专题北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100083, Peoples R China
2.Chinese Acad Prevent Med, Inst Virol, Beijing 100262, Peoples R China
推荐引用方式
GB/T 7714
Ju, XD,Lou, SQ,Wang, WG,et al. Effect of hydroxyurea and etoposide on transduction of human bone marrow mesenchymal stem and progenitor cell by adeno-associated virus vectors[J]. ACTA PHARMACOLOGICA SINICA,2004,25(2):196-202.
APA Ju, XD,Lou, SQ,Wang, WG,Peng, JQ,&Tian, H.(2004).Effect of hydroxyurea and etoposide on transduction of human bone marrow mesenchymal stem and progenitor cell by adeno-associated virus vectors.ACTA PHARMACOLOGICA SINICA,25(2),196-202.
MLA Ju, XD,et al."Effect of hydroxyurea and etoposide on transduction of human bone marrow mesenchymal stem and progenitor cell by adeno-associated virus vectors".ACTA PHARMACOLOGICA SINICA 25.2(2004):196-202.
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