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学科主题: 基础医学
题名:
Differential thymosin beta 10 expression levels and actin filament organization in tumor cell lines with different metastatic potential
作者: Liu, CR; Ma, CS; Ning, JY; You, JF; Liao, SL; Zheng, J
关键词: thymosin beta 10 ; tumor cell metastatic potential ; cell motility ; actin skeleton
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2004-02-01
卷: 117, 期:2, 页:213-218
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: HUMAN BREAST-CANCER ; BETA-THYMOSINS ; MOTILITY ; OVEREXPRESSION ; POLYMERIZATION
英文摘要:

Background To investigate the differential expression levels of thymosin beta10 ( Tbeta10) and the corresponding changes of actin filament organization in human tumor cell lines with different metastatic potential.

Methods Four groups of nine human tumor cell lines with different metastatic potential were analyzed for the amount of Tbeta10 mRNAs by Northern blot and for their peptide expression levels by immunohistochemistry. The filamentous actin ( F-actin) was observed by staining of TRITC-phalloidin to detect changes in actin organization.

Results In comparison with non-/weakly metastatic counterparts, Tbeta10 was upregulated in highly metastatic human lung cancer, malignant melanoma and breast cancer cell lines. Staining of TRITC-phalloidin revealed less actin bundles, a fuzzy network of shorter filaments and some F-actin aggregates in the highly metastatic tumor cells. Meanwhile, the actin filaments were robust and orderly arranged in the non-/weakly metastatic cancer cell lines.

Conclusion Tbeta10 levels correlate positively with the metastatic capacity in human tumors currently examined. The increasing metastatic potential of tumor cells is accompanied by a loss of F-actin, poorly arranged actin skeleton organizations and presence of F-actin aggregates. There is a consistent correlation between the elevated Tbeta10 expression and the disrupted actin skeleton.

语种: 英语
WOS记录号: WOS:000220107900011
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60434
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China

Recommended Citation:
Liu, CR,Ma, CS,Ning, JY,et al. Differential thymosin beta 10 expression levels and actin filament organization in tumor cell lines with different metastatic potential[J]. CHINESE MEDICAL JOURNAL,2004,117(2):213-218.
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