IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Expression of growth hormone receptor and its mRNA in hepatic cirrhosis
Wang, HT; Chen, S; Wang, J; Ou, QJ; Liu, C; Zheng, SS; Deng, MH; Liu, XP
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2003-04-01
9期:4页:765-770
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]ORTHOTOPIC LIVER-TRANSPLANTATION ; PLACEBO-CONTROLLED TRIAL ; TUMOR-NECROSIS-FACTOR ; RAT-LIVER ; HEPATOCELLULAR-CARCINOMA ; MALNOURISHED PATIENTS ; ENERGY-EXPENDITURE ; NUTRITIONAL-STATUS ; PROTEIN-SYNTHESIS ; MESSENGER-RNA
英文摘要

AIM: To investigate the expression of growth hormone receptor (GHR) and mRNA of GHR in cirrhotic livers of rats with the intension to find the basis for application of recombinant human growth hormone (rhGH) to patients with liver cirrhosis.

METHODS: Hepatic cirrhosis was induced in Sprague-Dawley rats by administration of thioacetamide intraperitoneally for 9-12 weeks. Collagenase IV was perfused in situ for isolation of hepatocytes. The expression of GHR and its mRNA in cirrhotic livers was studied with radio-ligand binding assay, RT-PCR and digital image analysis.

RESULTS: One class of specific growth hormone-binding site, GHR, was detected in hepatocytes and hepatic tissue of cirrhotic livers. The binding capacity of GHR (R-T, fmol/mg protein) in rat cirrhotic liver tissue (30.8 +/- 1.9) was significantly lower than that in normal control (74.9 +/- 3.9) at the time point of the ninth week after initiation of induction of cirrhosis (n=10, P<0.05), and it decreased gradually along with the accumulation of collagen in the process of formation and development of liver cirrhosis (P<0.05). The number of binding sites (x10(4)/cell) of GHR on rat cirrhotic hepatocytes (0.86 +/- 0.16) was significantly lower than that (1.28 +/- 0.24) in control (n=10, P<0.05). The binding affinity of GHR among liver tissue, hepatocytes of various groups had no significant difference (P>0.05). The expression of GHR mRNA (riOD, pixel) in rat cirrhotic hepatic tissues (23.3 +/- 3.1) was also significantly lower than that (29.3 +/- 3.4) in normal control (n=10, P<0.05).

CONCLUSION: The growth hormone receptor was expressed in a reduced level in liver tissue of cirrhotic rats, and lesser expression of growth hormone receptors was found in a later stage of cirrhosis. The reduced expression of growth hormone receptor was partly due to its decreased expression on cirrhotic hepatocytes and the reduced expression of its mRNA in cirrhotic liver tissue.

语种英语
WOS记录号WOS:000182469000029
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60445
Collection北京大学深圳医院
作者单位1.Peking Univ, Shenzhen Cent Hosp, Dept Gen Surg, Shenzhen 518036, Peoples R China
2.Sun Yat Sen Univ, Affiliated Hosp 2, Dept Hepatobiliary Surg, Sun Yat Sen Mem Hosp, Guangzhou 510120, Guangdong Prov, Peoples R China
3.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Gen Surg, Guangzhou 510630, Guangdong Prov, Peoples R China
4.Sun Yat Sen Univ, Sun Yat Sen Med Coll, Mol Med Lab Ctr, Guangzhou 510089, Guangdong Prov, Peoples R China
5.Sun Yat Sen Univ, Affiliated Hosp 2, Sun Yat Sen Mem Hosp, Dept Surg Gastroenterol, Guangzhou 510120, Guangdong Prov, Peoples R China
Recommended Citation
GB/T 7714
Wang, HT,Chen, S,Wang, J,et al. Expression of growth hormone receptor and its mRNA in hepatic cirrhosis[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(4):765-770.
APA Wang, HT.,Chen, S.,Wang, J.,Ou, QJ.,Liu, C.,...&Liu, XP.(2003).Expression of growth hormone receptor and its mRNA in hepatic cirrhosis.WORLD JOURNAL OF GASTROENTEROLOGY,9(4),765-770.
MLA Wang, HT,et al."Expression of growth hormone receptor and its mRNA in hepatic cirrhosis".WORLD JOURNAL OF GASTROENTEROLOGY 9.4(2003):765-770.
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