|Expression of growth hormone receptor and its mRNA in hepatic cirrhosis|
|Wang, HT; Chen, S; Wang, J; Ou, QJ; Liu, C; Zheng, SS; Deng, MH; Liu, XP|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||ORTHOTOPIC LIVER-TRANSPLANTATION ; PLACEBO-CONTROLLED TRIAL ; TUMOR-NECROSIS-FACTOR ; RAT-LIVER ; HEPATOCELLULAR-CARCINOMA ; MALNOURISHED PATIENTS ; ENERGY-EXPENDITURE ; NUTRITIONAL-STATUS ; PROTEIN-SYNTHESIS ; MESSENGER-RNA|
AIM: To investigate the expression of growth hormone receptor (GHR) and mRNA of GHR in cirrhotic livers of rats with the intension to find the basis for application of recombinant human growth hormone (rhGH) to patients with liver cirrhosis.
METHODS: Hepatic cirrhosis was induced in Sprague-Dawley rats by administration of thioacetamide intraperitoneally for 9-12 weeks. Collagenase IV was perfused in situ for isolation of hepatocytes. The expression of GHR and its mRNA in cirrhotic livers was studied with radio-ligand binding assay, RT-PCR and digital image analysis.
RESULTS: One class of specific growth hormone-binding site, GHR, was detected in hepatocytes and hepatic tissue of cirrhotic livers. The binding capacity of GHR (R-T, fmol/mg protein) in rat cirrhotic liver tissue (30.8 +/- 1.9) was significantly lower than that in normal control (74.9 +/- 3.9) at the time point of the ninth week after initiation of induction of cirrhosis (n=10, P<0.05), and it decreased gradually along with the accumulation of collagen in the process of formation and development of liver cirrhosis (P<0.05). The number of binding sites (x10(4)/cell) of GHR on rat cirrhotic hepatocytes (0.86 +/- 0.16) was significantly lower than that (1.28 +/- 0.24) in control (n=10, P<0.05). The binding affinity of GHR among liver tissue, hepatocytes of various groups had no significant difference (P>0.05). The expression of GHR mRNA (riOD, pixel) in rat cirrhotic hepatic tissues (23.3 +/- 3.1) was also significantly lower than that (29.3 +/- 3.4) in normal control (n=10, P<0.05).
CONCLUSION: The growth hormone receptor was expressed in a reduced level in liver tissue of cirrhotic rats, and lesser expression of growth hormone receptors was found in a later stage of cirrhosis. The reduced expression of growth hormone receptor was partly due to its decreased expression on cirrhotic hepatocytes and the reduced expression of its mRNA in cirrhotic liver tissue.
|作者单位||1.Peking Univ, Shenzhen Cent Hosp, Dept Gen Surg, Shenzhen 518036, Peoples R China|
2.Sun Yat Sen Univ, Affiliated Hosp 2, Dept Hepatobiliary Surg, Sun Yat Sen Mem Hosp, Guangzhou 510120, Guangdong Prov, Peoples R China
3.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Gen Surg, Guangzhou 510630, Guangdong Prov, Peoples R China
4.Sun Yat Sen Univ, Sun Yat Sen Med Coll, Mol Med Lab Ctr, Guangzhou 510089, Guangdong Prov, Peoples R China
5.Sun Yat Sen Univ, Affiliated Hosp 2, Sun Yat Sen Mem Hosp, Dept Surg Gastroenterol, Guangzhou 510120, Guangdong Prov, Peoples R China
|Wang, HT,Chen, S,Wang, J,et al. Expression of growth hormone receptor and its mRNA in hepatic cirrhosis[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(4):765-770.|
|APA||Wang, HT.,Chen, S.,Wang, J.,Ou, QJ.,Liu, C.,...&Liu, XP.(2003).Expression of growth hormone receptor and its mRNA in hepatic cirrhosis.WORLD JOURNAL OF GASTROENTEROLOGY,9(4),765-770.|
|MLA||Wang, HT,et al."Expression of growth hormone receptor and its mRNA in hepatic cirrhosis".WORLD JOURNAL OF GASTROENTEROLOGY 9.4(2003):765-770.|