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Identification and functional perspective of a novel HLA-A allele: A*0279
Liu, Fangfang; Wang, Shan; Ye, Yingjiang; Zhang, Huagang; Zhang, Yu; Chen, Weifeng
关键词HLA-A supertype cytotoxic T lymphocytes elispot assay crystal structure
刊名IMMUNOGENETICS
2006-06-01
DOI10.1007/s00251-006-0087-4
58期:5-6页:339-346
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Genetics & Heredity ; Immunology
研究领域[WOS]Genetics & Heredity ; Immunology
关键词[WOS]CLASS-I MOLECULES ; T-CELL EPITOPES ; PEPTIDE-BINDING ; CTL RECOGNITION ; SUPERTYPE ; ANTIGEN ; VIRUS ; SUPERMOTIFS ; SUBTYPES ; IMMUNOGENICITY
英文摘要

A novel HLA-A allele, HLA-A*0279, was identified using PCR-SSP and PCR-SBT methods. It is inheritable. HLA-A*0279 differs from HLA-A*020601 by a single nucleotide at position 497 in exon 3, leading to an amino acid change from Threonine to Isoleucine at the alpha2 helix of HLA molecule. To investigate whether the altered amino acid residue could affect its peptide-binding repertoire, we compared the predicted crystal structure of HLA-A*020601 and HLA-A*0279 by Swiss-PdbViewer software analysis. We found that the crystal structure of the two molecules is very similar except for a difference in the number of hydrogen bonds they can possibly form, which in turn could affect their structural stability. To test whether HLA-A*0279 has the ability to cross-present A*0201 - restricted peptides to T cells, the full lenght cDNA of HLA-A*0201, -A* 020601 and -A*0279 were respectively transfected into COS-7 cells, which were then used as targets in IFN-gamma release Elispot assay. A*2079 was found to be able to present A*0201- restricted peptides to and induce the response of CTL, thus it can be classified as member of the HLA-A2 functional supertype family. This finding would benefit the design of peptide vaccines to be applied in broader populations.

语种英语
WOS记录号WOS:000238014900002
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60485
专题北京大学第二临床医学院_胃肠外科
作者单位1.Peking Univ, Peoples Hosp, Surg Oncol Lab, Dept Gastrointestinal Surg, Beijing 100044, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China
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Liu, Fangfang,Wang, Shan,Ye, Yingjiang,et al. Identification and functional perspective of a novel HLA-A allele: A*0279[J]. IMMUNOGENETICS,2006,58(5-6):339-346.
APA Liu, Fangfang,Wang, Shan,Ye, Yingjiang,Zhang, Huagang,Zhang, Yu,&Chen, Weifeng.(2006).Identification and functional perspective of a novel HLA-A allele: A*0279.IMMUNOGENETICS,58(5-6),339-346.
MLA Liu, Fangfang,et al."Identification and functional perspective of a novel HLA-A allele: A*0279".IMMUNOGENETICS 58.5-6(2006):339-346.
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