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学科主题: 临床医学
题名:
Identification and functional perspective of a novel HLA-A allele: A*0279
作者: Liu, Fangfang; Wang, Shan; Ye, Yingjiang; Zhang, Huagang; Zhang, Yu; Chen, Weifeng
关键词: HLA-A supertype ; cytotoxic T lymphocytes ; elispot assay ; crystal structure
刊名: IMMUNOGENETICS
发表日期: 2006-06-01
DOI: 10.1007/s00251-006-0087-4
卷: 58, 期:5-6, 页:339-346
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Genetics & Heredity ; Immunology
研究领域[WOS]: Genetics & Heredity ; Immunology
关键词[WOS]: CLASS-I MOLECULES ; T-CELL EPITOPES ; PEPTIDE-BINDING ; CTL RECOGNITION ; SUPERTYPE ; ANTIGEN ; VIRUS ; SUPERMOTIFS ; SUBTYPES ; IMMUNOGENICITY
英文摘要:

A novel HLA-A allele, HLA-A*0279, was identified using PCR-SSP and PCR-SBT methods. It is inheritable. HLA-A*0279 differs from HLA-A*020601 by a single nucleotide at position 497 in exon 3, leading to an amino acid change from Threonine to Isoleucine at the alpha2 helix of HLA molecule. To investigate whether the altered amino acid residue could affect its peptide-binding repertoire, we compared the predicted crystal structure of HLA-A*020601 and HLA-A*0279 by Swiss-PdbViewer software analysis. We found that the crystal structure of the two molecules is very similar except for a difference in the number of hydrogen bonds they can possibly form, which in turn could affect their structural stability. To test whether HLA-A*0279 has the ability to cross-present A*0201 - restricted peptides to T cells, the full lenght cDNA of HLA-A*0201, -A* 020601 and -A*0279 were respectively transfected into COS-7 cells, which were then used as targets in IFN-gamma release Elispot assay. A*2079 was found to be able to present A*0201- restricted peptides to and induce the response of CTL, thus it can be classified as member of the HLA-A2 functional supertype family. This finding would benefit the design of peptide vaccines to be applied in broader populations.

语种: 英语
WOS记录号: WOS:000238014900002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60485
Appears in Collections:北京大学第二临床医学院_胃肠外科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Surg Oncol Lab, Dept Gastrointestinal Surg, Beijing 100044, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Liu, Fangfang,Wang, Shan,Ye, Yingjiang,et al. Identification and functional perspective of a novel HLA-A allele: A*0279[J]. IMMUNOGENETICS,2006,58(5-6):339-346.
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