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学科主题: 临床医学
题名:
Activation of gamma-aminobutyric Acid (A) Receptor Protects Hippocampus from Intense Exercise-induced Synapses Damage and Apoptosis in Rats
作者: Ding, Yi; Xie, Lan; Chang, Cun-Qing; Chen, Zhi-Min; Ai, Hua
关键词: Endoplasmic Reticulum Stress-induced Apoptosis ; gamma-aminobutyric Acid (A) Receptor ; Hippocampus ; Intense Exercise ; Synapse Plasticity
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2015-09-05
DOI: 10.4103/0366-6999.163392
卷: 128, 期:17, 页:2330-2339
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: STRESS-INDUCED APOPTOSIS ; GABA-RECEPTORS ; NMDA ; SYNAPTOPHYSIN ; STIMULATION ; PLASTICITY ; ISCHEMIA ; MUSCIMOL ; MORPHINE ; NEURONS
英文摘要:

Background: Our previous study has confirmed that one bout of exhaustion (Ex) can cause hippocampus neurocyte damage, excessive apoptosis, and dysfunction. Its initial reason is intracellular calcium overload in hippocampus triggered by N-methyl-D-aspartic acid receptor (NMDAR) over-activation. NMDAR activation can be suppressed by gamma-aminobutyric acid (A) receptor (GABA(A)R). Whether GABA(A)R can prevent intense exercise-induced hippocampus apoptosis, damage, or dysfunction will be studied in this study.

Methods: According to dose test, rats were randomly divided into control (Con), Ex, muscimol (MUS, 0.1 mg/kg) and bicuculline (BIC, 0.5 mg/kg) groups, then all rats underwent once swimming Ex except ones in Con group only underwent training. Intracellular free calcium concentration ([Ca2+]i) was measured by Fura-2-acetoxymethyl ester; glial fibrillary acidic protein (GFAP) and synaptophysin (SYP) immunofluorescence were also performed; apoptosis were displayed by dUTP nick end labeling (TUNEL) stain; endoplasmic reticulum stress-induced apoptosis pathway was detected by Western blotting analysis; Morris water maze was used to detect learning ability and spatial memory.

Results: The appropriate dose was 0.1 mg/kg for MUS and 0.5 mg/kg for BIC. Ex group showed significantly increased [Ca2+]i and astrogliosis; TUNEL positive cells and levels of GFAP, B cell lymphoma-2 (Bcl-2) associated X protein (Bax), caspase-3, caspase-12 cleavage, CCAAT/enhancer binding protein homologous protein (CHOP), and p-Jun amino-terminal kinase (p-JNK) in Ex group also raised significantly compared to Con group, while SYP, synapse plasticity, and Bcl-2 levels in Ex group were significantly lower than those in Con group. These indexes were back to normal in MUS group. BIC group had the highest levels of [Ca2+]i, astrogliosis, TUNEL positive cell, GFAP, Bax, caspase-3, caspase-12 cleavage, CHOP, and p-JNK, it also gained the lowest SYP, synapse plasticity, and Bcl-2 levels among all groups. Water maze test showed that Ex group had longer escape latency (EL) and less quadrant dwell time than Con group; all indexes between MUS and Con groups had no significant differences; BIC had the longest EL and least quadrant dwell time among all groups.

Conclusions: Activation of GABA(A)R could prevent intense exercise-induced synapses damage, excessive apoptosis, and dysfunction of hippocampus.

语种: 英语
所属项目编号: 30270636 ; 30671015
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000361746700011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60487
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China

Recommended Citation:
Ding, Yi,Xie, Lan,Chang, Cun-Qing,et al. Activation of gamma-aminobutyric Acid (A) Receptor Protects Hippocampus from Intense Exercise-induced Synapses Damage and Apoptosis in Rats[J]. CHINESE MEDICAL JOURNAL,2015,128(17):2330-2339.
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